Anti‐diabetic and gut microbiota modulation effects of sacha inchi (Plukenetia volubilis L.) leaf extract in streptozotocin‐induced type 1 diabetic mice

Abstract

BACKGROUND Sacha inchi (Plukenetia volubilis L.) tea has been used as an adjuvant treatment for diabetes in Pu’er, in the Yunnan province of China. The effects of sacha inchi tea on diabetes and the underlying mechanisms remain unknown. This study was conducted to investigate the influence of a water extract of sacha inchi (P. volubilis L.) leaves (PWE) on hypoglycemic activity and gut microbiota composition in mice with streptozotocin (STZ)‐induced type 1 diabetes mellitus (T1DM). During the 6 weeks of the study, T1DM mice were administered PWE intragastrically at 400 mg kg−1 body weight (BW) per day. RESULTS Treatment with PWE reduced excessive loss of BW and excessive intake of food. It significantly decreased blood glucose levels and improved oral glucose tolerance. The treatment caused protective histopathological transformations in sections of the pancreas, leading to decreased insulin resistance and improved insulin sensitivity. Treatment with PWE also significantly ameliorated disorders of the gut microbiota structure and increased the richness and diversity of intestinal microbial species in T1DM mice. At the genus level, the populations of several crucial bacteria, such as Akkermansia, Parabacteroides, and Muribaculum increased in the PWE treatment group but the abundance of Ruminiclostridium and Oscillibacter decreased. CONCLUSIONS Treatment with PWE can ameliorate hyperglycemic symptoms in STZ‐induced T1DM mice, and the anti‐diabetic effect of PWE was related to the amelioration of gut microbial structural disorder and the enrichment of functional bacteria. © 2022 Society of Chemical Industry.

Surrogate fostering of mice prevents prenatal estradiol-induced insulin resistance via modulation of the microbiota-gut-brain axis

“…According to published studies, a drop in Alloprevotella ( Wang J. et al, 2020 ; Wu et al, 2021 ; Zhang et al, 2021 ; Zhao et al, 2021 ; Hao et al, 2022 ; Liu et al, 2022 ) and an increase in Blautia ( Zhao et al, 2021 ; Bao et al, 2022 ) and Tyzzerella ( Li et al, 2022 ) are found in animal models of in insulin resistance or glucose intolerance. On the other hand, antidiabetic medications and bioactive compounds modulate the gut microbial community of diabetic mouse model, characterized by increased Alistipes ( Hu et al, 2019 ; Jeong et al, 2021 ; Li et al, 2021 ; Wu R. et al, 2022 ) and decreased Anaerotruncus ( Yong et al, 2022 ), Oscillibacter ( Lin et al, 2022 ; Wang et al, 2022 ), Allobaculum ( Jia et al, 2017 ; Shen et al, 2021 ; Xu et al, 2021 ; Ma et al, 2022 ), Acetatifactor ( Wu Y. et al, 2022 ) and Turicibacter ( Zhao et al, 2021 ). These findings coincide with the correlation between microbiota and metabolic phenotypes in our study.…”

<em>Pueraria thomsonii </em>Radix Water Extract Alleviates Type 2 Diabetes Mellitus in <em>db/db </em>Mice through the Integrated Regulation of Metabolism and Gut Microbiota

“…In animal models of experimental diabetes, the species and number of beneficial bacteria were significantly lower, while pathogenic bacteria were significantly higher [17].In this study,we found that PTR significantly upregulated the abundance of Muribaculum, norank_f__Muribaculaceae, and Parabacteroides in db/db mice while significantly reducing the abundance of the harmful bacterium Desulfovibrio. A study has demonstrated that Plukenetia volubilis L. leaf significantly increased Muribaculum abundance in db/db mice, a beneficial bacterium that can improve inflammation, dyslipidemia, and glucose intolerance [18][19].Norank_f_Muribaculaceae was a beneficial dominant bacterium in the mouse intestine and was negatively correlated with the glucose levels of postprandial blood [20]. Parabacteroides is a beneficial bacterium that significantly improves insulin resistance and has been shown in vitro to activate the intestinal gluconeogenic pathway through the conversion of succinate, thereby exerting a hypoglycaemic effect [21].…”

Pueraria thomsonii Radix Water Extract Alleviate Type 2 Diabetes Mellitus in db/db Mice through Comprehensive Regulation of Metabolism and Gut Microbiota

“…In animal models of experimental diabetes, the species and number of beneficial bacteria were significantly lower, while pathogenic bacteria were significantly higher [23]. A study has demonstrated that the Plukenetia volubilis L. leaf significantly increased Muribaculum abundance in db/db mice, a beneficial bacterium that can improve inflammation, dyslipidemia, and glucose intolerance [24,25]. In this study, we found that PTR significantly upregulated the abundance of Muribaculum, Norank_f_Muribaculaceae, and Parabacteroides in db/db mice, while significantly reducing the abundance of the harmful bacterium Desulfovibrio.…”