Anti-diabetic Activity of Convallatoxin Isolated from the Root Bark of Parquetina nigrescens (Afzel.) Bullock (Asclepiadaceae)

Faloye, Kolade Olatubosun; Ayoola, Marcus Durojaye; Amos-Tautua, Bamidele; Famuyiwa, Samson O.

doi:10.9734/ejmp/2018/45129

Cited by 11 publications

(8 citation statements)

References 23 publications

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“…The extract at100, 200 and 400 mg/kg was devoid of activity at 0.5-2 h but at 4 h it showed hyperglycaemia lowering effect comparable to glibenclamide (5 mg/kg) thus indicating only insulin stimulation as its mechanism of action (Table 1). Similar effects have been reported for the extracts of Parquetina nigrescens and Uvaria afzelii [26,31].…”

Section: Glucose Lowering Activity Of the Extractsupporting

confidence: 87%

Isolation and Characterization of Two New Polyphenols from the Anti-Diabetic Fraction of Pod Extract of Caesalpinia pulcherrima Swartz

Faloye

Famuyiwa

Ayoola

et al. 2020

EJMP

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This study was planned and executed to validate the anti-diabetic ethno-medicinal claim of Caesalpinia pulcherrima (Fabaceae) pods and the isolation of potential chemical compounds responsible for the activity.The anti-diabetic activity of the extract was assayed using oral glucose tolerance test and streptozotocin induced hyperglycaemic rats. The partition fractions of the extract were evaluated for their anti-hyperglycaemic activity using oral glucose tolerance test. The most active fraction was subjected to chromatographic separations that led to the isolation and characterisation of two new polyphenolic compounds. The structures of these compounds were elucidated and characterized using IR, 1D- and 2D-NMR and MS techniques.The extract gave comparable (p>0.05) activity to glibenclamide (5 mg/kg) at 100, 200 and 400 mg/kg at 4 h in oral glucose tolerance test and streptozotocin induced diabetic model on day 21. The most active ethyl acetate fraction (200 mg/kg) elicited comparable activity to the positive control at 0.5-4 h with blood glucose reduction of 52.9 % as compared with glibenclamide (5 mg/kg) of 38.9 % at 4 h. The isolated compounds were identified to be 5-(4-hydroxyphenyl)-3-hydroxy-2-methoxyphenol and 3-(4-methanetriol-2,6-dihydroxyphenyl)-3',4',5',5,7-pentahydroxyflavanonol.The significant anti-diabetic property shown by the pods of C. pulcherrima justified its anti-diabetic ethno-medicinal use and the two new polyphenolic compounds isolated from its most active fraction could have contributed to the observed activity.

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Section: Glucose Lowering Activity Of the Extractsupporting

confidence: 87%

Isolation and Characterization of Two New Polyphenols from the Anti-Diabetic Fraction of Pod Extract of Caesalpinia pulcherrima Swartz

Faloye

Famuyiwa

Ayoola

et al. 2020

EJMP

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“…They suggested that phytol and squalene isolated from the methanol leaf extract may be responsible for the improvement in the function of the kidney in diabetic mellitus possibly by increasing urea synthesis in urine metabolism and increasing the excretion of urea. Faloye and colleagues [ 50 ] conducted research on the methanol root bark extract of P. nigrescens and the convallatoxin compound isolated from P. nigrescens root bark. Administration of the methanolic root extract to streptozotocin-induced diabetic rats at a dose of 200 mg/kg body weight showed a significant dose and time-dependent reduction in blood glucose levels that was comparable to the standard control glibenclamide at 5 mg/kg for 4 hours.…”

Section: Resultsmentioning

confidence: 99%

“A Review of Parquetina nigrescens (Afzel.) Bullock, A Plant for Traditional Medicine: Phytochemical and Pharmacological Properties”

Adase

Ankutse²,

Kumadoh

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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The development of herbal medicines as a remedy for several illnesses has been recognized and accepted worldwide by health experts. Parquetina nigrescens is a perennial evergreen woody climber from the Apocynaceae family, widely used in Africa for the treatment of many diseases. The current study is intended to review and put together information available on this ethno-medicinal plant, which will improve scientific knowledge about the plant and also identify research areas that need to be investigated further. The information related to the plant was obtained using scientific databases such as Google scholar, WebMD, Wiley, Science direct, Cochrane database, student thesis, PubMed, and Scopus to obtain relevant literature regarding the botanical descriptions and distribution of the plant, traditional uses, phytochemicals, active compounds isolated from the plant, and pharmacological properties of P. nigrescens. Several traditional uses for different parts of the plant (leaves, stem bark, roots, leaf sap, flowers, and latex) have been presented. A review of the phytochemical composition of different plant parts revealed the presence of reducing sugars, flavonoids, tannins, alkaloids, cardiac glycosides, steroids, coumarin, anthraquinones, terpenoids, and saponins. Many studies also highlighted pharmacological activities related to P. nigrescens, including antianemic and haematological activity, antidiabetic, anti-inflammatory, antipyretic, analgesic, antiasthmatic, antimicrobial, insecticidal, neurotoxic, cytotoxic, antityphoid, antipolycystic ovarian syndrome activity, antilipidemic, and memory-enhancing activity. It is recommended that further in-vivo and clinical studies be conducted on the plant for the development of novel drugs.

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“…Singlet at δ 3.02 indicated single hydrogen (H-17) of C-17 of ring D, proving substitution at C-17 and singlet at δ 0.84 exposed 3 hydrogens of methyl group (C-18) attached to C-13. Double doublet at δ 4.72 and singlet at δ 5.59 were characteristic peaks for H-21 and H-22 of five membered lactone ring attached to steroid moiety (Faloye et al, 2018;McIntyre et al, 1990).…”

Section: Proton-nmr Spectrum Analysismentioning

confidence: 99%

Proton NMR and HR-LC/MS based phytochemical analysis of methanolic fraction of Alectra parasitica A. Rich. rhizomes

Patil

2020

Heliyon

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Introduction: Alectra parasitica (Scrophulariaceae/Orobanchaceae) is a rarely occurring parasitic plant grows on roots of Vitex negundo L. (Verbenaceae). As per Indian system of medicine, Ayurveda, it can be used in treatment of various diseases. So far, this plant has not been explored phyto-chemically in detail. Non-small cell lung cancer (NSCLC) is mostly occurring type of lung cancer, which so far can be treated by chemotherapy approach including cisplatin. Aim: Present research work was aimed towards preparation of methanolic fraction of A. parasitica rhizomes; its phytochemical analysis by 1H-NMR and HR-LC/MS; evaluation of its anti-cancer property against NSCLC-A549 cell line. Methods: For preparation of methanolic fraction (MF), A. parasitica rhizome powder was defatted; extracted with combination of water and alcohol (1:1); added with lead acetate and then sulphuric acid; fractionation of ethyl acetate fraction with methanol. After phytochemical analysis of MF by preliminary chemical testing, TLC, 1 H-NMR and HR-LC/MS techniques, MF was screened for its anti -cancer property against NSCLC-A549 cell line by MTT assay. Results: Detail phytochemical analysis reflected successful preparation of tannin-less MF of A. parasitica rhizomes. Different types of analytical techniques first time proved the proved the presence of various types of phytochemicals in this plant. On MTT assay, it was found that MF has anti-cancer property against NSCLC-A549 cell line with IC 50 value, 306.51 μg/ml. Conclusion:MF contains different phytochemicals like iridoids, flavonoids, steroid glycosides and also strigalactones; which cumulatively exert anti-cancer effect on A549. Appearance of all these compounds is significant in chemotaxonomic surveillance of this rare plant and specially, strigalactones can be proved important in establishing their parasitism with host plant.

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Anti-diabetic Activity of Convallatoxin Isolated from the Root Bark of Parquetina nigrescens (Afzel.) Bullock (Asclepiadaceae)

Cited by 11 publications

References 23 publications

Isolation and Characterization of Two New Polyphenols from the Anti-Diabetic Fraction of Pod Extract of Caesalpinia pulcherrima Swartz

Isolation and Characterization of Two New Polyphenols from the Anti-Diabetic Fraction of Pod Extract of Caesalpinia pulcherrima Swartz

“A Review of Parquetina nigrescens (Afzel.) Bullock, A Plant for Traditional Medicine: Phytochemical and Pharmacological Properties”

Proton NMR and HR-LC/MS based phytochemical analysis of methanolic fraction of Alectra parasitica A. Rich. rhizomes

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