Anti-desmoglein 1 antibodies in healthy related and unrelated subjects and patients with pemphigus foliaceus in endemic and non-endemic areas from Tunisia

Abida, Olfa; Kallel-Sellami, M.; Joly, P.; Ayed, M. Ben; Zitouni, M.; Masmoudi, Abderrahmen; Mokni, M.; Fezzaa, B.; Osman, A. Ben; Kammoun,; Gilbert, Danièle; Turki, H.; Tron, François; Masmoudi, Hatem; Makni, S.

doi:10.1111/j.1468-3083.2009.03265.x

Cited by 29 publications

(36 citation statements)

References 21 publications

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“…The onset and course of PF depend on both genetic predisposing and exogenous inducing factors. The involvement of environmental factors is supported by several arguments (i) normal individuals living in the endemic areas of Tunisia [17] and Brazil [18] may possess antiDsg1 Abs [19] (ii) a significant association between farming and the presence of these auto-Abs has been reported (iii) epidemiological study demonstrated that Tunisian endemic PF was significantly [20], and (iv) finally, the potential effect of parasites such as leishmania and hydatidosis in the aetiopathogenesis of endemic PF has been suggested [21,22].…”

Section: Introductionmentioning

confidence: 95%

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Potential Role of TLR Ligand in Aethiopathogenesis of Tunisian Endemic Pemphigus Foliaceus

D¹

2013

Biochem Physiol

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Pemphigus foliaceus (PF) is an autoimmune skin disease in which environmental factors are thought to participate. Recent studies suggest that microbial components use signaling molecules of the human Toll-like receptor (TLR) family to transduce signals in keratinocytes. The aim of our research was to investigate the expression of TLRs 2, 3 and 4 by keratinocytes of PF patients compared to normal keratinocytes, in order to characterise the nature of the microbial factor involved in the etiopathology of PF. Biopsies obtained from 43 PF patients and 20 healthy controls were assessed by immunohistochemical analysis using specific polyclonal antibodies. The TLR2, TLR3 and TLR4 expression was significantly upregulated in PF epidermis. The significant increase of those TLRs simultaneously may merely reflect the complicated environmental conditions of rural women in the southern rural regions of Tunisia. Interestingly, we have found that the TLR4 diffuse expression was associated with the production of anti-desmoglein 1 Abs (p=0.037). This could be in line with a potential role of TLR ligand in aethiopathogenesis of Tunisian endemic PF.TLR over-expression in pemphigus skin indicates that TLRs are involved in the pathogenesis of pemphigus through stimulation by infectious or endogenous ligands.

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Section: Introductionmentioning

confidence: 95%

“…In patient group, anti-Dsg 1 antibodies were detected by a commercial enzyme-linked immunosorbent assay kit (MBL, Nagoya, Japan) as reported previously [17].…”

Section: Correlation Between Tlr Expression and Autoantibody Titersmentioning

confidence: 99%

Potential Role of TLR Ligand in Aethiopathogenesis of Tunisian Endemic Pemphigus Foliaceus

D¹

2013

Biochem Physiol

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“…It is worth noting that the Tunisian endemic form of PF constitutes a distinct variety of the disease with particular and unique epidemiological characteristics since the disease occurs mainly in young females at a mean age of 35 yet it spares children [12,13].…”

Section: Introductionmentioning

confidence: 99%

Polymorphisms of HLA microsatellite marker in Tunisian pemphigus foliaceus

et al. 2013

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“…Characteristics of the Tunisian endemic form of PF include a significantly higher incidence in southern regions as compared with northern regions [6, 7]. The observation that the majority of PF family cases in Tunisia were also located in the southern regions implies the role of genetic susceptibility or environmental factors, and DRB1*03 was concluded to be the main susceptibility allele for the Tunisian endemic PF in previous studies [8, 9].…”

Section: Introductionmentioning

confidence: 99%

Autoimmune reactivity against precursor form of desmoglein 1 in healthy Tunisians in the area of endemic pemphigus foliaceus

Toumi

Saleh

Yamagami

et al. 2013

Journal of Dermatological Science

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Background Desmoglein 1 (Dsg1), the pemphigus foliaceus (PF) antigen, is produced as a precursor (preDsg1) and is transported to the cell surface as the mature form (matDsg1). Recent studies show that B cells from North American individuals without pemphigus can potentially produce anti-preDsg1 IgG antibodies, but ELISA screening of large numbers of normal people in North America and Japan hardly ever shows circulating antibodies against preDsg1 or matDsg1. In contrast, in Tunisia, where PF is endemic, anti-Dsg1 IgGs are frequently detected in healthy individuals. Objective To characterize these anti-Dsg1 antibodies from normal individuals in Tunisia. Methods Sera from 16 healthy individuals and 9 PF patients in the endemic PF area in Tunisia, and sera from Japanese non-endemic PF patients were analyzed by immunoprecipitation-immunoblotting using recombinant proteins of preDsg1, matDsg1, and domain-swapped Dsg1/Dsg2 molecules. Results Sera from normal Tunisian individuals reacted to preDsg1 alone (8/16) or more strongly to preDsg1 than to matDsg1 (7/16), while those from all Tunisian PF patients and Japanese non-endemic PF patients reacted similarly to preDsg1 and matDsg1, or preferentially to matDsg1. The epitopes recognized by anti-Dsg1 IgGs from normal Tunisian individuals were more frequently found in the C-terminal extracellular domains (EC3 to EC5), while those in Tunisian endemic PF patients were more widely distributed throughout the extracellular domains, suggesting IgGs against EC1 and EC2 developed during disease progression. Conclusions These findings indicate that IgG autoantibodies against Dsg1 are mostly raised against preDsg1 and/or C-terminal domains of Dsg1 in healthy Tunisians in the endemic area of PF.

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Anti-desmoglein 1 antibodies in healthy related and unrelated subjects and patients with pemphigus foliaceus in endemic and non-endemic areas from Tunisia

Abstract: These results show that the endemic features of Tunisian pemphigus foliaceus are focused in these southern areas more than in other areas and that both environmental and genetic factors contribute to the disease.

Cited by 29 publications

References 21 publications

Potential Role of TLR Ligand in Aethiopathogenesis of Tunisian Endemic Pemphigus Foliaceus

Potential Role of TLR Ligand in Aethiopathogenesis of Tunisian Endemic Pemphigus Foliaceus

Polymorphisms of HLA microsatellite marker in Tunisian pemphigus foliaceus

Autoimmune reactivity against precursor form of desmoglein 1 in healthy Tunisians in the area of endemic pemphigus foliaceus

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