Anti-colitis effect of Lactobacillus sakei K040706 via suppression of inflammatory responses in the dextran sulfate sodium-induced colitis mice model

Seo, Seunghwan; Shin, Ji‐Sun; Lee, Wooseok; Rhee, Young Kyoung; Cho, Chang‐Won; Hong, Hee‐Do; Lee, Kyung‐Tae

doi:10.1016/j.jff.2016.12.045

Cited by 39 publications

(32 citation statements)

References 76 publications

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“…Similar increases in pro-inflammatory mediators are also observed in the DSS-colitis model correlating well with clinical parameters [ 66 ]. Consistent with the known anti-inflammatory effects of some probiotic strains [ 72 , 73 , 74 , 75 ], we found that both probiotic strategies (PP and LGG) significantly reduced mRNA levels of Il-1β and Il-6 in the colons of treated mice, which were accompanied by increased anti-inflammatory cytokine expression ( Il-10 and TGF-β ). Interestingly, the personalized probiotic group showed a more effective immune modulation as compared to the commercial probiotic LGG, suggesting that resident microbes of the gut may yield stronger anti-inflammatory effects since they already possess a mutualistic anti-inflammatory relationship with the host’s immune system.…”

Section: Discussionsupporting

confidence: 87%

Isolation and Characterization of Potentially Probiotic Bacterial Strains from Mice: Proof of Concept for Personalized Probiotics

et al. 2018

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Modulation of the gut microbiota through the use of probiotics has been widely used to treat or prevent several intestinal diseases. However, inconsistent results have compromised the efficacy of this approach, especially in severe conditions such as inflammatory bowel disease (IBD). The purpose of our study was to develop a personalized probiotic strategy and assess its efficacy in a murine model of intestinal inflammation. Commensal bacterial strains were isolated from the feces of healthy mice and then administered back to the host as a personalized treatment in dextran sodium sulfate (DSS)-induced colitis. Colonic tissues were collected for histological analysis and to investigate inflammatory markers such as Il-1β, Il-6, TGF-β, and Il-10, and the enzyme myeloperoxidase as a neutrophil marker. The group that received the personalized probiotic showed reduced susceptibility to DSS-colitis as compared to a commercial probiotic. This protection was characterized by a lower disease activity index and reduced histopathological damage in the colon. Moreover, the personalized probiotic was more effective in modulating the host immune response, leading to decreased Il-1β and Il-6 and increased TGF-β and Il-10 expression. In conclusion, our study suggests that personalized probiotics may possess an advantage over commercial probiotics in treating dysbiotic-related conditions, possibly because they are derived directly from the host’s own microbiota.

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Section: Discussionsupporting

confidence: 87%

Isolation and Characterization of Potentially Probiotic Bacterial Strains from Mice: Proof of Concept for Personalized Probiotics

et al. 2018

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“…Although many Lactobacillus strains have been widely used as probiotics, only a few studies have reported the probiotic properties of L. sakei strains [36][37][38]. In our current study, L. sakei ob4.1 reduced the in ammatory cytokine levels both in vitro and in vivo.…”

Section: Discussionsupporting

confidence: 51%

“…In order to identify the roles of L. sakei ob4.1 strain in host colon epithelium, we focused on the antiin ammatory properties of L. sakei ob4.1 in colon epithelial cells. The characteristics of L. sakei are diverse across the strains, with only a few strains showing anti-in ammatory properties [36][37][38]. In comparative genomic analysis, L. sakei ob4.1 had the highest similarity with L. sakei proBio65 strain ( Supplementary Fig.…”

Section: Sakei Ob41 Strain Reduced Palmitate-induced In Ammation mentioning

confidence: 99%

High-fat diet-induced oxidative stress links increased colonisation of Lactobacillus sakei with its anti-inflammatory properties in obesity

Lee

Bae

Kim

et al. 2020

Preprint

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Abstract Background: Obesity is one of the major public health problems related to various chronic health conditions, with steadily increasing prevalence worldwide. Lactobacillus provides various benefits to the host body; however, its role in obesity is unknown. Results: In this study, we found higher colonisation of Lactobacillus sakei species in obesity group, which in turn was related to increased reactive oxygen species (ROS) levels induced by higher fat intake. We isolated L. sakei ob4.1 strain from the faeces of one subject with obesity and compared its genetic and molecular features with those of L. sakei DSM 20017 strain. L. sakei ob4.1 showed higher catalase activity, which was regulated by oxidative stress at gene transcriptional levels. L. sakei ob4.1 maintained colon epithelial cell-adhesion ability under ROS stimulation, and live bacteria could decrease colon epithelial inflammation in a dose-dependent manner. Establishing a mouse model revealed high-fat diet-induced colon ROS to be associated with increased colonisation of L. sakei ob4.1 through catalase activity. Four-week supplementation with this strain could reduce colon inflammation effectively, though not so for body weight or ROS levels in high fat-fed mice. Conclusion: We, therefore, concluded that changes in host gut-oxidative stress levels could link high fat-induced obesity to increased colonisation of L. sakei ob4.1 , and this strain could be potent as anti-inflammatory probiotic in obese population with gut inflammation. Keywords Lactobacillus sakei; Obesity; Gut microbiome; Reactive oxygen stress; Inflammation; High fat diet

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“…Several other strains of Lactobacillus plantarum exhibited therapeutic effects on gastrointestinal inflammation through their modulatory functions against inflammatory cytokines[ 37 ]. A recent study indicated that Lactobacillus sakei attenuated the clinical symptoms and histological damage by suppressing inflammatory mediators, such as NF-κB, STAT1, and TL4[ 38 ]. A combined therapy consisting of Lactobacillus casei , butyrate, and Pistacia atlantica significantly improved histological scores and reduced MPO activity in a rat model of IBD[ 39 ].…”

Section: Diets and Dietary Interventions For Ibdmentioning

confidence: 99%

Diets, functional foods, and nutraceuticals as alternative therapies for inflammatory bowel disease: Present status and future trends

Mijan

Lim

2018

WJG

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Inflammatory bowel disease (IBD) is a serious health concern among western societies. The disease is also on the rise in some East Asian countries and in Australia. Health professionals and dietitians around the world are facing an unprecedented challenge to prevent and control the increasing prevalence of IBD. The current therapeutic strategy that includes drugs and biological treatments is inefficient and are associated with adverse health consequences. In this context, the use of natural products is gaining worldwide attention. In vivo studies and clinical evidence suggest that well-planned dietary regimens with specific nutrients can alleviate gastrointestinal inflammation by modulating inflammatory cytokines, such as tumor necrosis factor α (TNF-α), interleukin 1 (IL-1), IL-6, IL-1β, and IL-10. Alternatively, the avoidance of high-fat and high-carbohydrate diets is regarded as an effective tool to eliminate the causes of IBD. Many functional foods and bioactive components have received attention for showing strong therapeutic effects against IBD. Both animal and human studies suggest that bioactive functional foods can ameliorate IBD by downregulating the pro-inflammatory signaling pathways, such as nuclear factor κB, STAT1, STAT6, and pro-inflammatory cytokines, including IL-1β, IL-4, IL-6, COX-2, TNF-α, and interferon γ. Therefore, functional foods and diets have the potential to alleviate IBD by modulating the underlying pathogenic mechanisms. Future comprehensive studies are needed to corroborate the potential roles of functional foods and diets in the prevention and control of IBD.

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Anti-colitis effect of Lactobacillus sakei K040706 via suppression of inflammatory responses in the dextran sulfate sodium-induced colitis mice model

Cited by 39 publications

References 76 publications

Isolation and Characterization of Potentially Probiotic Bacterial Strains from Mice: Proof of Concept for Personalized Probiotics

Isolation and Characterization of Potentially Probiotic Bacterial Strains from Mice: Proof of Concept for Personalized Probiotics

High-fat diet-induced oxidative stress links increased colonisation of Lactobacillus sakei with its anti-inflammatory properties in obesity

Diets, functional foods, and nutraceuticals as alternative therapies for inflammatory bowel disease: Present status and future trends

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