Anti-coagulase-negative Staphylococcus activity of ethanolic extracts of propolis from two Brazilian regions and synergism with antimicrobial drugs by the E-test method

Mantovani, Rebeca Passarelli; Rall, Vera Lúcia Mores; Batalha, Jackson E. N.; Fernandes, Adalton Mazetti

doi:10.1590/s1678-91992008000200012

Cited by 6 publications

(6 citation statements)

References 21 publications

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“…Mantovani et al [46] demonstrated strong anti-CoNS activity of propolis. Najmadeen and Kakamand studies [47] on ethanol extracts of propolis activities on S. epidermidis and S. aureus showed that S. epidermidis strains were less susceptible to different propolis extracts than coagulase positive staphylococci.…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, in this study all S. epidermidis strains MIC EEPP ranged from 0.2 to 0.39 mg/mL and from 0.39 to 0.78 mg/mL after 12 and 24 hours of incubation, respectively, which suggested that EEPP activity might diminish over time. The determination of the chemical characteristics of EEP showed that the phenolic compounds were mainly responsible for the anti-CoNS activity of EEP collected from the Southeast of Brazil [46]. The findings of another study, evaluating the antibacterial properties of extracts of propolis from Mexico, revealed that the highest sensitivity towards propolis was shown by S. aureus , S. epidermidis, and the two Vibrio cholerae strains with MICs values <0.125 mg/mL [49].…”

Section: Discussionmentioning

confidence: 99%

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In VitroAntimicrobial Activity of Ethanolic Extract of Polish Propolis against Biofilm FormingStaphylococcus epidermidisStrains

Wojtyczka

Kępa

Idzik

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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The aim of the presented study was to examine the antimicrobial activity of ethanol extract of Polish propolis (EEPP) against biofilm-forming CoNS strains in vitro. Our results revealed that EEPP displayed varying degrees of activity against CoNS with MIC values ranging from 1.56 to 0.78 mg/mL. The average MIC was 1.13 ± 0.39 mg/mL while calculated MIC50 and MIC90 values were 0.78 mg/mL and 1.56 mg/mL, respectively. The biofilm formation ability by all tested S. epidermidis strains was inhibited at EEPP concentrations ranging from 0.39 to 1.56 mg/mL. The degree of reduction of AlamarBlue was directly associated with the proliferation of S. epidermidis strains. The increased proliferation of S. epidermidis strains was observed after 12 and 24 hours of incubation in the presence of EEPP concentrations ranging from 0.025 to 0.39 mg/mL. These results suggest that antimicrobial activities of EEPP against S. epidermidis expressed as the reduction of bacterial growth, reduction of biofilm formation ability, and the intensity of proliferation were significantly affected by incubation time and EEPP concentration used as well as the interactions between these factors.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

In VitroAntimicrobial Activity of Ethanolic Extract of Polish Propolis against Biofilm FormingStaphylococcus epidermidisStrains

Wojtyczka

Kępa

Idzik

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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“…Colored zones produced on a pale background indicated positive result for detection of alcohols, phenols, and steroids compounds [7].…”

Section: Spraying Methodsmentioning

confidence: 99%

Antimicrobial Activity and Chemical Screening of Propolis Extracts

Fatima¹,

Baserisalehi²,

Bahador³

2014

AJLS

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Abstract:Propolis is a resinous mixture that collects by honey bees from the plants. However, the physical character of propolis generally has been used by honey bees to protect their hive, but several beneficial properties of this compound could be considered for human being. In the present study, two propolis samples (P1&P2) were collected and subjected for extraction using different solvents. Then, their antimicrobial effects were evaluated against Salmonella typhi PTCC 1609, Pseudomonas aeruginosa PTCC 1047, Staphylococcus aureus PTCC 1112, Escherichia coli PTCC 1338, Bacillus cereus PTCC 1015, Aspergillus niger PLM 1140 and Candida albicans ATCC 1405 using Well Diffusion Agar. In addition, the bioactive compounds and functional groups of the extracts were determined by paper chromatography and Spray methods. The results obtained indicated that ethanol and methanol extracts of the propolis showed relatively more antimicrobial effect and both extracts exhibited similar responses against the antagonistic microorganisms. Bacillus cereus, Salmonella typhi , Escherichia coli and Staphylococcus aureus were sensitive whereas, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger were resistant to the extracts. E.coli was sensitive to methanol and resistant to ethanol extracts. Our finding concerning to the chemical analysis of the propolis exhibited the presence of flavonoid, tannin, steroid, alcohol and alkaloid in extracts. Overall, propolis has antimicrobial effect with different spectrum and therefore, it might consider a potent candidate for treatment of several clinical scenarios.

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“…However, ethanolic extract and essential oil of propolis present different chemical compositions (20). Probably, diverse extraction procedures result in dissimilar chemical compounds, and ultimately, could contribute to differences in the antibacterial activities (29,30). It is not so clear whether the antibacterial effect may be caused by a single active component or by the synergy of many active constituents found in the essential oil.…”

Section: Antibacterial Activitymentioning

confidence: 99%

Chemical composition and antibacterial activity of Brazilian propolis essential oil

Oliveira¹,

França²,

Kuster³

et al. 2010

J. Venom. Anim. Toxins incl. Trop. Dis

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ABSTRACT:The present study aimed at investigating the chemical composition of essential oil extracted from Brazilian propolis and the susceptibility of Staphylococcus aureus, Staphylococcus epidermides, Streptococcus pyogenes and Escherichia coli to this substance. The essential oil was obtained by steam distillation of propolis and examined by gas chromatography/mass spectrometry (GC/MS). In addition, the agar diffusion method using filter paper disks was employed. Antibacterial activity was measured as equivalent diameters of inhibition zones (in millimeters) after incubation at 37C for 24 hours. From the 26 identified constituents, β-caryophyllene (12.7%), acetophenone (12.3%) and β-farnesene (9.2%) were found to be major components. New components, namely linalool, methyl hydrocinnamate, ethyl hydrocinnamate, α-ylangene, γ-elemene and valencene, are reported for the first time to be present in propolis essential oil. This oil also exhibited antibacterial activity.

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Anti-coagulase-negative Staphylococcus activity of ethanolic extracts of propolis from two Brazilian regions and synergism with antimicrobial drugs by the E-test method

Cited by 6 publications

References 21 publications

In VitroAntimicrobial Activity of Ethanolic Extract of Polish Propolis against Biofilm FormingStaphylococcus epidermidisStrains

In VitroAntimicrobial Activity of Ethanolic Extract of Polish Propolis against Biofilm FormingStaphylococcus epidermidisStrains

Antimicrobial Activity and Chemical Screening of Propolis Extracts

Chemical composition and antibacterial activity of Brazilian propolis essential oil

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