Anti-cervical cancer activity of secondary metabolites of endophytic fungi from Ginkgo biloba

He, Qing; Zeng, Qiangcheng; Shao, Yibo; Zhou, Haixia; Li, Tianjiao; Song, Fang; Liu, Wei

doi:10.3233/cbm-190462

Cited by 17 publications

(7 citation statements)

References 14 publications

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“…In our study, of 46 endophytic fungi strains investigated, 23 extracts (50%) showed cytotoxicity more or less, amongst 21 extracts (46.65%) were cytotoxic against A549 cells, 16 extracts (34.78%) were cytotoxic against Hela cells, 21 extracts (46.65 %) were cytotoxic against HepG cells. That were in accordance with the previous reports, of which 9 endophytic fungi were successfully obtained from the leaves of Ginkgo biloba, the extracts of isolates J-1, J-2 and J-3 markedly inhibited the proliferation of HeLa cells, promoted their apoptosis and blocked their migration 29 and 12 isolates extracts (85.7%) derived from mangrove Rhizophora mucronata were cytotoxic (cell viability < 50%) against T47D cells 5 .…”

Section: Discussionsupporting

confidence: 91%

Antimicrobial and Cytotoxic Potential of Crude Extracts from Culturable Fungal Endophytes Associated with the Mangrove Species Rhizophora stylosa and R. mucronata in the South China Sea

Xu¹,

Zhou²

2021

Preprint

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Our research evaluated the antimicrobial and cytotoxic activities of crude extracts of endophytic fungi from R. stylosa and R. mucronate. 46 fungal isolates were cultured on the four different mediums, namely Dextrose Agar (PDA), Czapek’s Agar (CZA), Rice Medium (RM) and Grain Medium (GM), and harvested by ethyl acetate solvent at 40 days. The extracts were tested for antimicrobial activity by the microdilution method against Gram-negative bacteria Pseudomons adaceae (PA), Gram-positive bacteria Enterococcus faecalis (EF), Methicillin-resistant Staphylococcus aureus (MRSA) and pathogenic fungus Monilia albican (MA). Cytotoxic activity of the extracts was evaluated by MTT assay using human lung cancer cells A549, human cervical carcinoma cells Hela, and human hepatocellular cells HepG2. The results showed that rice medium could promote the secretion of antimicrobial and anti-tumor secondary metabolites of endophytic fungi in comparison with the other cultivation media. 17 strains (68%) from R. stylosa exhibited inhibition effects on indicators, especially N. protearum HHL46 which could inhibit the growth of four microbes with the MIC values reaching 0.0625 mg/mL. 15 strains (71.4%) from R. mucronata displayed activities against human pathogenic microbes, especially Pestalotiopsis sp. HQD6 and N. protearum HQD5 which could resist the growth of four microbial with MIC values ranging from 0.015 to 1 mg/mL. In cytotoxic assay, the extracts of 10 strains (40%), 9 strains (40%) and 13 strains (52%) from R. stylosa and 13 strains (61.9%), 10 stains (47.6%) and 10 stains (47.6%) in R. mucronata displayed the cytotoxicity against A549, Hela and HepG2 cancer cells with cells viability value ≤50%, respectively. Neopestalotiopsis protearum HHL46, Phomopsis longicolla HHL50, Botryosphaeria fusispora HQD83, Fusarium verticillioides HQD48 and Pestalotiopsis sp. HQD6 displayed significant antitumor activity with IC50 values below the 20 μg/mL. These results highlighted the antimicrobial and anti-tumor potential of endophytic fungi from R. stylosa and R. mucronata and the possibility to be exploited for their antimicrobial and cytotoxic agents.

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Section: Discussionsupporting

confidence: 91%

Antimicrobial and Cytotoxic Potential of Crude Extracts from Culturable Fungal Endophytes Associated with the Mangrove Species Rhizophora stylosa and R. mucronata in the South China Sea

Xu¹,

Zhou²

2021

Preprint

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“…These secondary metabolites demonstrated marked inhibition of HeLa cell proliferation, promoted their apoptosis, and blocked their migration. Furthermore, these metabolites significantly attenuated the growth of HeLa tumors implanted in mice [ 121 ].…”

Section: Mycobiome and Cancer Therapymentioning

confidence: 99%

The Mycobiome: Cancer Pathogenesis, Diagnosis, and Therapy

Gamal

Elshaer

Alabdely

et al. 2022

Cancers

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Cancer is among the leading causes of death globally. Despite advances in cancer research, a full understanding of the exact cause has not been established. Recent data have shown that the microbiome has an important relationship with cancer on various levels, including cancer pathogenesis, diagnosis and prognosis, and treatment. Since most studies have focused only on the role of bacteria in this process, in this article we review the role of fungi—another important group of the microbiome, the totality of which is referred to as the “mycobiome”—in the development of cancer and how it can impact responses to anticancer medications. Furthermore, we provide recent evidence that shows how the different microbial communities interact and affect each other at gastrointestinal and non-gastrointestinal sites, including the skin, thereby emphasizing the importance of investigating the microbiome beyond bacteria.

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“…[1][2][3] It can be used to treat many diseases, such as cardiovascular disease, 4 kidney disease, 5 neurodegenerative diseases, [6][7][8] hyperlipidemia, 9 and tumors. [10][11][12] Ginkgolide B (GB, Figure 1A) is the most active component among its terpenoid extracts and is the most potent platelet-activating factor receptor antagonist. 13,14 But GB has numerous limitations in preparing dosage forms, including its almost insolubility in water, 15,16 rapid elimination in vivo, limited bioavailability, 17,18 and unstable long-term storage.…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetics of ginkgolide B–lyophilized nanoparticles after intravenous injection in rats using liquid chromatography–tandem mass spectrometry

Zhu

Peng

et al. 2023

Rapid Comm Mass Spectrometry

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Rationale Ginkgolide B (GB) performs diverse pharmacological activities but has poor water solubility. The currently available GB injections have a short half‐life and are lethal when injected rapidly. We prepared GB‐lyophilized nanoparticles (GB‐NPs) using a new nonsurfactant polysaccharide polymer, ZY‐010, as its carrier to regulate the release of GB in vivo. Here, the pharmacokinetics (PK) of GB‐NPs after intravenous injection in rats was performed using liquid chromatography–tandem mass spectrometry (LC–MS/MS). Methods The samples were separated on an Agilent Eclipse XDB‐C 18 column (2.1 × 50 mm, 1.85 μm) maintained at 30°C. The MS/MS transitions of GB and glibenclamide as the internal standard (IS) were set at m/z 423.1 → 367.1 and m/z 492.1 → 367.0, respectively. The standard curve of GB content was constructed, and the specificity, sensitivity, precision, and extraction recovery of LC–MS/MS analysis were assessed. The main PK parameters were analyzed using DAS (Drug And Statistics for Windows) software, version 2.0. Results The retention time of GB and IS at elution was 2.77 and 4.75 min, respectively. An excellent linear response across the concentration range of 0.001–100 μg/ml was achieved (r = 0.9997). The relative standard deviation value of precision was less than 10%. The total extraction recovery was above 80.76 ± 2.08%. The main PK parameters for the GB‐NPs were as follows: t1/2 = 69.32 h, AUC(0 → ∞) = 188 312.97 ± 143 312.41 μg/L h, CL = 0.03 ± 0.02 L/h/kg, and V = 0.09 ± 0.05 L/kg. The t1/2 of the GB‐NPs was significantly longer than that of GB solution, and AUC(0 → ∞) of GB‐NPs was about 1.4 times that of GB solution. The PK data demonstrated that the blood concentration of GB in rats conformed to a three‐compartment model in both GB solution and GB‐NPs. Conclusion A rapid and accurate LC–MS/MS method was established for the determination of GB‐NPs in rats. GB‐NPs exhibited a sustained‐release behavior in vivo compared with GB solution.

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Anti-cervical cancer activity of secondary metabolites of endophytic fungi from Ginkgo biloba

Cited by 17 publications

References 14 publications

Antimicrobial and Cytotoxic Potential of Crude Extracts from Culturable Fungal Endophytes Associated with the Mangrove Species Rhizophora stylosa and R. mucronata in the South China Sea

Antimicrobial and Cytotoxic Potential of Crude Extracts from Culturable Fungal Endophytes Associated with the Mangrove Species Rhizophora stylosa and R. mucronata in the South China Sea

The Mycobiome: Cancer Pathogenesis, Diagnosis, and Therapy

Pharmacokinetics of ginkgolide B–lyophilized nanoparticles after intravenous injection in rats using liquid chromatography–tandem mass spectrometry

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