Anti-cell Proliferative and Anti-angiogenic Potential of Andrographolide During 7,12-Dimethylbenz(a)anthracene Induced Hamster Buccal Pouch Carcinogenesis

Singh, Arjun; Manoharan, Shanmugam; Vasudevan, K; Rajasekaran, D; Manimaran, Asokan; Suresh, K.

doi:10.7314/apjcp.2013.14.10.6001

Cited by 9 publications

(6 citation statements)

References 36 publications

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“…These findings are consistent with those of other investigators [29,31]. The current findings, combined with those from other investigations [29,32,33], supported the idea that DMBA-induced HBP carcinoma go through the same alterations as in human. Many of the structural changes seen in carcinogen-treated HBP mucosa, both at the gross and light microscopic levels, are quite similar to those seen in human oral cancer development.…”

Section: -Discussionsupporting

confidence: 93%

Effect of Docosahexaenoic Acid as a Chemopreventive Agent on Experimentally Induced Hamster Buccal Pouch Carcinogenesis

Alqalshy¹,

Ibrahim²,

Abdel-Hafiz³

et al. 2022

Preprint

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The purpose of the current study was directed to investigate the effectiveness of docosahexaenoic acid (DHA) as a chemopreventive agent on experimentally induced hamster buccal pouch (HBP) carcinogenesis; Material and methods: 40 Syrian male hamsters, five weeks old, were divided into 4 groups of 10 animals in each as follows, GI: Topical application of liquid paraffin alone (thrice a week for 14 weeks), GII: Topical application of 7, 12 dimethyl benz[a]anthracene (DMBA) alone (0.5 % in liquid paraffin, thrice a week for 14 weeks), GIII: Topical application of DMBA (0.5 % in liquid paraffin, thrice a week for 14 weeks) + Oral administration of DHA (125 mg/kg b.w. in 1 ml distilled water by oral gavage, thrice a week for 14 weeks on alternative days of DMBA application), GIV : Oral administration of DHA alone (125 mg/kg b.w. in 1 ml distilled water by oral gavage, thrice a week for 14 weeks); Results: Gross observations and histopathological findings revealed a-GI: normal stratified squamous epithelium b- GII: well and moderately differentiated squamous cell carcinoma (SCC) c-: GIII: showed variable results ranges from hyperkeratosis, hyperkeratosis and focal hyperplasia, mild dysplasia, and well differentiated SCC with superficial invasion of tumor cells not extended to deeper areas d: GIV: normal similar to GI. Immunohistochemical results revealed that oral DHA treatment to DMBA treated hamsters restored the normal expression of bcl-2; Conclusion: DHA has the potential to be a dietary chemopreventive agent due to its capacity to improve carcinogen detoxification and to block/suppress the initiation and promotion stages of experimentally produced HBP carcinogenesis.

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Section: -Discussionsupporting

confidence: 93%

Effect of Docosahexaenoic Acid as a Chemopreventive Agent on Experimentally Induced Hamster Buccal Pouch Carcinogenesis

Alqalshy¹,

Ibrahim²,

Abdel-Hafiz³

et al. 2022

Preprint

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“…On the other hand, the increase in growth of small vessels in pre-cancer and cancer, which is characterized by rapid and chaotic sprouting of new, usually immature vessels, serves to provide nutrients to the proliferating cancer cells (25). Overexpression of VEGF has been observed in DMBA-treated hamster oral mucosa (26,27) as a marker of angiogenesis (28,29), suggesting that the changes we observed here are due in part to increasing VEGF over the course of neoplastic progression. Upregulation of other pro-angiogenic growth factors and endothelial receptor tyrosine kinases such as Tie2 likely contribute to these vascular abnormalities as well (30).…”

Section: Discussionsupporting

confidence: 53%

Distinct Angiogenic Changes during Carcinogenesis Defined by Novel Label-Free Dark-Field Imaging in a Hamster Cheek Pouch Model

Martin

Martinez

et al. 2017

Cancer Research

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There remain gaps in knowledge concerning how vascular morphology evolves during carcinogenesis. In this study, we imaged neovascularization by label-free dark field microscopy of a DMBA-induced hamster cheek pouch model of oral squamous cell carcinoma (SCC). Wavelength-dependent imaging revealed distinct vascular features at different imaging depths and vessel sizes. Vascular tortuosity increased significantly in high-risk lesions, while diameter decreased significantly in hyperplastic and SCC lesions. Large vessels preserved the same trends seen in the original images, whereas small vessels displayed different trends, with length and diameter increasing during carcinogenesis. Based on these data we developed and validated a classification algorithm incorporating vascular features from different vessel masks. Receiver operator curves generated from the classification results demonstrated high accuracies in discriminating normal and hyperplasia from high-grade lesions (area under the curve>0.95). Overall, these results provided automated imaging of vasculature in the earliest stages of carcinogenesis from which one can extract robust endpoints. The optical toolbox described here is simple, low-cost and portable and can be used in a variety of health care and research settings for cancer prevention and pharmacology research.

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“…Andrographolide is the active constituent of Andrographic paniculata (Table 1) with anti-inflammatory effects and also targeting NF-κB (Dai et al, 2011;Luo et al, 2013;Trivedi, Rawal, & Patel, 2007;Wang et al, 2013). Singh et al have proved the anticell proliferative and anti angiogeneic potential of andrographolide (Singh et al, 2013). Andrographolide was reported to induce apoptosis via inactivation of STAT3 and Akt pathway and potentiate antitumor activity of gemcitabine in pancreatic cancer (Bao et al, 2013).…”

Section: Discussionmentioning

confidence: 97%

Protease activated receptor-2 (PAR2): possible target of phytochemicals

Kakarala

Jamil

2014

Journal of Biomolecular Structure and Dynamics

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The use of phytochemicals either singly or in combination with other anticancer drugs comes with an advantage of less toxicity and minimal side effects. Signaling pathways play central role in cell cycle, cell growth, metabolism, etc. Thus, the identification of phytochemicals with promising antagonistic effect on the receptor/s playing key role in single transduction may have better therapeutic application. With this background, phytochemicals were screened against protease-activated receptor 2 (PAR2). PAR2 belongs to the superfamily of GPCRs and is an important target for breast cancer. Using in silico methods, this study was able to identify the phytochemicals with promising binding affinity suggesting their therapeutic potential in the treatment of breast cancer. The findings from this study acquires importance as the information on the possible agonists and antagonists of PAR2 is limited due its unique mechanism of activation.

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Anti-cell Proliferative and Anti-angiogenic Potential of Andrographolide During 7,12-Dimethylbenz(a)anthracene Induced Hamster Buccal Pouch Carcinogenesis

Cited by 9 publications

References 36 publications

Effect of Docosahexaenoic Acid as a Chemopreventive Agent on Experimentally Induced Hamster Buccal Pouch Carcinogenesis

Effect of Docosahexaenoic Acid as a Chemopreventive Agent on Experimentally Induced Hamster Buccal Pouch Carcinogenesis

Distinct Angiogenic Changes during Carcinogenesis Defined by Novel Label-Free Dark-Field Imaging in a Hamster Cheek Pouch Model

Protease activated receptor-2 (PAR2): possible target of phytochemicals

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