Anti-Candida Activity of Biosurfactant Produced by Rhodotorula paludigena

Halvaeezadeh, Marzieh; Mahmoudabadi, Ali Zarei

doi:10.2174/1573408013666161219150524

Cited by 5 publications

(6 citation statements)

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“…Mahmoudabadi were shown that the produced biosurfactant by R. paludigena in combination with caspofungin have synergistic effect against C. albicans strains [26].…”

Section: Resultsmentioning

confidence: 99%

“…Producing biosurfactants by fungi are limited to some species of Candida, Pseudozyma, Yarrowia, Penicillium and Aspergillus species [5,[20][21][22][23]. Recently, the production of biosurfactants from Rhodotorula glutinis [24], R. mucilaginosa [25] and R. paludigena [26] has been investigated by researchers. It seems that Rhodotorula species are mainly composed biosurfactants, hence they have new possibilities for industrial application.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation Laboratory Produced Biosurfactant by <em>Rhodotorula </em>Species and Its Antifungal Activity

Gharaghani¹,

Halvaeezadeh²,

Mahmoudabadi³

2019

Preprint

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Background: Biosurfactants are amphiphilic surface active compounds that produced by several microorganisms, including, bacteria and fungi. Biodegradability, low toxicity, applications diversity and functionality under extreme conditions are characterized them from chemically biosurfactants. It is found that, Rhodotorula species, read yeasts, have high potency for biosurfactant producing. Recently, antimicrobial activities of biosurfactants have been subjected for new antibiotic therapy. The aim of the present study was to evaluate biosurfactant production by the different strains of Rhodotorula species in laboratory conditions. In addition, antifungal activity of produced biosurfactant was assessed against several saprophytic fungi. In the present study 54 strains of Rhodotorula including, R. glutinis (48 strains), R. minuta (2 strains), R. mucilaginosa (2 strains) and Rhodotorula species (2 strains) were screened for biosurfactant production. The biosurfactant was produced using the Sabouraud dextrose broth medium and confirmed by specific tests. Antifungal assay was also evaluated by disk diffusion method and the serial dilutions of biosurfactant. In the present study, although all tested strains were capable to produce biosurfactant in vitro, the degree of biosurfactant production was varied among stains. 7.4% strains had the highest (+5) biosurfactant activity followed by 16.7%, 29.5%, 25.8% and 20.4% had +4, +3, +2 and +1, respectively. In the present study, all tested fungi were inhibited at 40 µl of biosurfactant. Rhodotorula species are appropriate organisms for the production of biosurfactants and R. glutinis strains have the greatest ability to producing biosurfactant than another species. Furthermore, our results were demonstrated that the produced biosurfactant by R. glutinis presented a valuable potential for biopharmaceutical applications.

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“…Mahmoudabadi were shown that the produced biosurfactant by R. paludigena in combination with caspofungin have synergistic effect against C. albicans strains [26].…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation Laboratory Produced Biosurfactant by <em>Rhodotorula </em>Species and Its Antifungal Activity

Gharaghani¹,

Halvaeezadeh²,

Mahmoudabadi³

2019

Preprint

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“…A synergistic effect of surfactin with ketoconazole against C. albicans was also considered by Liu et al (27). Furthermore, Halvaeezadeh and Zarei Mahmoudabadi showed that the produced biosurfactant by R. paludigena in combination with caspofungin have synergistic effects against C. albicans strains (26).…”

mentioning

confidence: 82%

“…Producing biosurfactants by fungi is limited to some species of Candida, Pseudozyma, Yarrowia, Penicillium and Aspergillus species (5,(20)(21)(22)(23). Recently, the production of biosurfactants from Rhodotorula glutinis (24), R. mucilaginosa (25), and R. paludigena (26) has been investigated by researchers. It seems that Rhodotorula species are the main producers of biosurfactants; hence, they have new possibilities for industrial application.…”

Section: Several Reports Indicated That Bacterial Species Such Asmentioning

confidence: 99%

“…The free cell supernatant was removed using centrifugation at 3000 g for 10 minutes. The same volume of supernatant (crude biosurfactant) and chloroform-methanol (2:1 v/v) were mixed, and then the biosurfactant was extracted by centrifugation (26,31). The gather biosurfactant was dried and kept at -20ºC until use.…”

Section: Biosurfactant Production On a Laboratory Scalementioning

confidence: 99%

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Evaluation of Laboratory-Produced Biosurfactant by Rhodotorula Species and Its Antifungal Activity

Gharaghani

Mahmoudabadi

Halvaeezadeh

2019

Jundishapur J Nat Pharm Prod

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Background: Biosurfactants are amphiphilic surface-active compounds that are produced by several microorganisms, including bacteria and fungi. Biodegradability, low toxicity, application diversity, and functionality under extreme conditions characterize them from chemical biosurfactants. It is found that Rhodotorula species, read yeasts, have high potency for biosurfactant production. Recently, antimicrobial activities of biosurfactants have been subjected to new antibiotic therapy. Objectives: The aim of the present study was to evaluate the biosurfactant production by the different strains of Rhodotorula species in laboratory conditions. In addition, the antifungal activity of produced biosurfactant was assessed against several saprophytic fungi. Methods: In the present study, 54 strains of Rhodotorula including R. glutinis (48 strains), R. minuta (two strains), R. mucilaginosa (two strains), and Rhodotorula species (two strains) were screened for biosurfactant production. The biosurfactant was produced in Sabouraud dextrose broth medium and confirmed by specific tests. The antifungal assay was carried out by a disk diffusion method using serial dilutions of biosurfactant. Results: In the present study, although all tested strains were capable of producing biosurfactant in vitro, the degree of biosurfactant production varied among the strains. 7.4% of the strains had the highest (+5) biosurfactant activity while 16.7%, 29.5%, 25.8%, and 20.4% had +4, +3, +2, and +1, respectively. In the present study, all tested fungi were inhibited at 40 µL of the biosurfactant. Conclusions: Rhodotorula species are appropriate organisms for the production of biosurfactants and R. glutinis strains have the greatest ability to produce biosurfactant among other species. Furthermore, our results demonstrated that the produced biosurfactant by R. glutinis presents a valuable potential for biopharmaceutical applications.

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