Anti-Cancerous Potential of Polysaccharide Fractions Extracted from Peony Seed Dreg on Various Human Cancer Cell Lines Via Cell Cycle Arrest and Apoptosis

Abstract

In this study, four homo/heterogenous polysaccharides (HBSS, CHSS, DASS, and CASS) extracted from peony seed dreg with respective molecular weights of 3467, 4677, 229, and 56 kDa were evaluated for anti-cancerous attributes in prostate cancer cells (Pc-3), colon cancer cells (HCT-116), human breast cancer cells (MCF-7), cervical cancer (Hela cells) and human embryonic kidney 293 (HEK 293) cells as control. Among them, CASS and DASS extracted by alkali, consisted of 34.43% Gal, 26.39% Ara, 21.80% Glc and 35.77% Ara, 19.35% Gal, 17.77% Man, respectively. CASS fraction had the most significant inhibitory effects on all the cell lines used whereas HBSS had least effect. The CASS shown remarkable inhibition and cytotoxic effects in Hela cells followed by other cell lines as compared to 5-fluorouracil (5-FU). CASS arrested cell cycle in G0/G1 phase except MCF-7 cells and increased apoptotic cells percentage varied in different treated cells. CASS down regulated the expression of Cyclin A/B1/D1/E1, CDK-1/2/4/6 and p15/16/21/27 excluding p53. The notable change in expression of proteins (Cytochrome C, Bax, Bcl-2, p-Caspase-3, -8, -9, and PARP) was observed followed by Apaf-1 and Survivin. These findings indicated that CASS has an anti-cancerous potential in the treatment of human cancers which make it a potent candidate in functional foods.

Thermal and Antioxidant Properties of Polysaccharides Sequentially Extracted from Mulberry Leaves (Morus alba L.)

“…Previously, our research group studied the thermal and rheology characteristics of plant-derived polysaccharides and their functional properties [ 14 , 23 , 24 , 25 , 40 ], but the study of the thermal and rheological characteristics of mulberry polysaccharides is still in its infancy. We reported the sequential extraction of MLPs with different solvents such as hot buffer, chelating agent, dilute alkali, and concentrated alkali, which led to considerable differences in yield and chemical composition.…”

“…Polysaccharides are a heterogeneous group of carbohydrates that consist of monosaccharide units with glycosidic linkages [ 13 ] with diverse structures and compositions, which can influence various functional properties. It has been reported that polysaccharides have exhibited various biological activities such as anti-tumor, anti-inflammatory, anti-bacterial, and immunologic activity, as well as anti-mutagenic efficacy [ 14 , 15 , 16 , 17 , 18 ]. Moreover, polysaccharides also play an important role as radical scavengers to prevent oxidative damage [ 19 ].…”

Effect of sugar types on structural and flavor properties of peony seed derived Maillard reaction products

“…After the extraction of peony seed oil, the protein‐enriched by‐product obtained is known as peony seed meal (Zhang et al, ). In the previous studies, peony seed protein hydrolysate was reported with appreciable physicochemical (Shi, Zhang, Sun, Xu, et al, ), antioxidant (Shi, Zhang, Sun, Qu, et al, ), and anticancerous properties (Wei, Ni, Thakur, Liao, Hu, et al, ; Zhang et al, ). Therefore, the economic value of peony seed meal can be realized through bioutilization of protein.…”

Pereskia bleo Leaves Extract Induces Cell Death via Cell Cycle Arrest and Apoptosis in Cervical Cancer Cells HeLa

“…Cancer is a condition that occurs when abnormal cells in the body proliferate uncontrollably causing normal cells to die (1). To date, cancer remains the primary cause of death in humans worldwide in spite of the advancement in tools for its diagnosis, treatment, and prevention (2).…”