Anti-Cancerous Effect of Rutin Against HPV-C33A Cervical Cancer Cells via G0/G1 Cell Cycle Arrest and Apoptotic Induction

Abstract

Background: Nowadays, the potential therapeutic role of various bioflavonoids including Curcumin, Luteolin and Resveratrol has currently been well-documented in a vast range of fatal complications including synaptic failure and cancers. These bioflavonoids are widely being implemented for the treatment of various cancers as they possess anti-cancerous, anti-oxidant and anti-inflammatory properties. Moreover, they are also used as a better alternative to conventional therapies since; these are non-toxic to cells and having no or least side effects. Notably, the pertinent therapeutic role of Rutin in cervical cancer is still unsettled however, its anti-cancerous role has already been reported in other cancers including prostate and colon cancer. Rutin (Vitamin P or Rutoside) is a polyphenolics flavonoid exhibiting multi-beneficial roles against several carcinomas. Objective: Despite the evidence for its several biological activities, the anticancer effects of Rutin on human cervical cancer (C33A) cells remain to be explored. In this study, the anticancer potential of Rutin was investigated by employing the key biomarkers such as nuclear condensation reactive oxygen species (ROS), apoptosis, and changes in mitochondrial membrane potential (MMP). Results: Our findings showed that Rutin treatment reduced the cell viability, induced significant increase in ROS production and nuclear condensation in dose-dependent manner. Moreover, Rutin provoked apoptosis by inducing decrease in MMP and activation of caspase-3. Cell cycle analysis further confirmed the efficacy of Rutin by showing cell cycle arrest at G0/G1 phase. Conclusion: Thus, our study is envisaged to open up interests for elucidating Rutin as an anticancerous agent against cervical cancer.

Rutin Modulates MAPK Pathway Differently from Quercetin in Angiotensin II-Induced H9c2 Cardiomyocyte Hypertrophy

“…The intake of the diet is reported to prevent cardiovascular diseases [ 15 ]. Currently, studies have been conducted to investigate the potential anticancer [ 16 , 17 , 18 , 19 ] and antiviral [ 20 , 21 , 22 ] properties of quercetin and rutin. In addition, combination therapy of quercetin and vitamin C has been reported to be beneficial for the prevention and early treatment of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2 or COVID-19) [ 23 ].…”

Antiproliferative Effects of Roylea cinerea (D. Don) Baillon Leaves in Immortalized L6 Rat Skeletal Muscle Cell Line: Role of Reactive Oxygen Species Mediated Pathway

“…Further, cell population in G0/G1 phase was increased in treated cells from 65.5% to 66.1% with a decrease in S phase (23.4% to 21.5%) and G1/M phase (7.6% to 7.3%). The results indicated the increase in apoptotic cells and cell cycle arrest at G0/G1 phase in treated cells as compared to the nontreated cells which may be attributed to DNA damage mediated p53 activation to check further cell proliferation (Figure 4E) (Khan et al, 2019). The anticancer drugs specifically act by initiating various signaling pathways and ultimately inducing apoptosis.…”

Rutinin Ratlarda Kolistin Kaynaklı Testis Hasarında Oksidatif DNA Hasarı, NF-κB Aracılı Enflamasyon ve Apoptoz Üzerindeki Koruyucu Etkileri

“…Yapılan bu çalışmada kaspaz 3 immunpozitifliğinin kontrol ve sadece Rut uygulanan grupta değişmediği, Kolistin ve Kolistin + Rut 50 uygulanan grupta önemli derecede arttığı, kolisitnle birlikte Rut 100 uygulaması sonrası ise bu immunpozitifliğin azaldığı gözlemlenmiştir. Rut uygulamasının kaspaz 3 seviyesini azalttığı ve apoptoz aktivitesini düşürdüğü önceki çalışmalarda rapor edilmiştir [42,[45][46][47][48][49]. Dolayısıyla bu çalışmadan elde edilen veriler önceki çalışmalarla uyumlu olarak bulunmuştur.…”