Anti-Cancer Properties of Flaxseed Proteome

Merkher, Yulia; Kontareva, Elizaveta; Alexandrova, Anastasia; Javaraiah, Rajesha; Pustovalova, Margarita; Leonov, Sergey

doi:10.3390/proteomes11040037

Cited by 3 publications

(3 citation statements)

References 164 publications

(214 reference statements)

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“…Flaxseed protein concentrate may be a good source of bioactive peptides having antibacterial, antioxidant, and antidiabetic effects as well as an angiotensin-converting enzyme inhibition effect due to the amino acid sequence of 11S globulin [15]. Merker et al [28] highlighted the potential of flaxseed proteins including 11S globulins to produce peptides with an anticarcinogenic effect.…”

Section: Influence Of Cultivar and Concentration Methods On Relative ...mentioning

confidence: 99%

“…According to other authors [3,25,28], these are polymeric proteins with MW ≈ 300 kDa. The PGs we have found have primary polypeptide sequences of 367-536 amino acids in length, from which two sub-polypeptide chains, an acidic and a basic one, are cut during post-translational processing, similarly to proteins of other oilseeds [40], which then give rise to the final monomer by their association via a disulfide bond.…”

Section: Seed Storage Proteinsmentioning

confidence: 96%

“…Other important flaxseed proteins are reported to be the 7S storage protein, oleosins, late embryogenesis abundant (LEA) proteins and a number of metabolically important enzymes [1,26]. The importance of flaxseed proteins, especially 11S globulins and 2S albumins, in relation to food applications, human nutrition and medical applications has been sufficiently discussed in recent works [6,15,[26][27][28].…”

Section: Introductionmentioning

confidence: 99%

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Proteomic Profile of Flaxseed (Linum usitatissimum L.) Products as Influenced by Protein Concentration Method and Cultivar

Jarošová,

Roudnický,

Bárta

et al. 2024

Foods

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The research is focused on the quantitative evaluation of the flaxseed (Linum usitatissimum L.) proteome at the level of seed cake (SC), fine flour—sieved a fraction below 250 µm (FF)—and protein concentrate (PC). The evaluation was performed on three oilseed flax cultivars (Agriol, Raciol, and Libra) with different levels of α-linolenic acid content using LC-MS/MS (shotgun proteomics) analysis, which was finalized by database searching using the NCBI protein database for Linum usitatissimum and related species. A total of 2560 protein groups (PGs) were identified, and their relative abundance was calculated. A set of 33 quantitatively most significant PGs was selected for further characterization. The selected PGs were divided into four classes—seed storage proteins (11S globulins and conlinins), oleosins, defense- and stress-related proteins, and other major proteins (mainly including enzymes). Seed storage proteins were found to be the most abundant proteins. Specifically, 11S globulins accounted for 41–44% of SC proteins, 40–46% of FF proteins, and 72–84% of PC proteins, depending on the cultivar. Conlinins (2S albumins) were the most abundant in FF, ranging from 10 to 13% (depending on cultivar). The second most important class from the point of relative abundance was oleosins, which were represented in SC and FF in the range of 2.1–3.8%, but only 0.36–1.20% in PC. Surprisingly, a relatively high abundance of chitinase was found in flax products as a protein related to defence and stress reactions.

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Section: Influence Of Cultivar and Concentration Methods On Relative ...mentioning

confidence: 99%

Section: Seed Storage Proteinsmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

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Proteomic Profile of Flaxseed (Linum usitatissimum L.) Products as Influenced by Protein Concentration Method and Cultivar

Jarošová,

Roudnický,

Bárta

et al. 2024

Foods

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Therapeutic implications of flaxseed peptides and bioactive components against various diseases

Imran,

Munir,

Altemimi

et al. 2024

Journal of Functional Foods

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High-frequency ultrasound-assisted drug delivery of chia, cress, and flax conjugated hematite iron oxide nanoparticle for sono-photodynamic lung cancer treatment in vitro and in vivo

Abd El-Kaream,

Zedan,

Mohamed

et al. 2024

Cancer Nano

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Background Sono-photodynamic therapy (SPDT), which combines photodynamic (PDT) and sonodynamic (SDT) therapies with sensitizers, offers new avenues for cancer treatment. Even though new sensitizers for SPDT have been synthesized with great success, few of them are effectively used. The limited tumor-targeting specificity, inability to transport the sensitizers deeply intratumorally, and the deteriorating tumor microenvironment limit their anti-tumor effectiveness. The current study was carried out aiming at high-frequency ultrasound-assisted drug delivery of chia, cress and flax conjugated hematite iron oxide nanoparticles (CCF–HIONP) for photothermal–photodynamic lung cancer (LCA) treatment in vitro and in vivo as activated cancer treatment up-to-date modality. Materials and methods The study was conducted in vitro on human LCA cells (A-549) and the study protocol application groups in vivo on Swiss albino mice treated with benzo[a]pyrene only and were not received any treatment for inducing LCA, and only after LCA induction the study treatment protocol began, treatment was daily with CCF–HIONP as HIFU–SPDT sensitizer with or without exposure to laser (IRL) or high-frequency ultrasound (HIFU–US) or a combination of laser and/or high-frequency ultrasound for 3 min for 2 weeks. Results Revealed that HIONP can be employed as effective CCF delivery system that directly targets LCA cells. In addition, CCF–HIONP is a promising HIFU–SPS for HIFU–SPDT and when combined with HIFU–SPDT can be very effective in treatment of LCA–A549 in vitro (cell viability decreased in a dose-dependent basis, the cell cycle progression in G0/G1 was slowed down, and cell death was induced as evidenced by an increase in the population of Pre-G cells, an increase in early and late apoptosis and necrosis, and an increase in autophagic cell death) and benzo[a]pyrene LCA-induce mice in vivo (decreased oxidative stress (MDA), and ameliorated enzymatic and non-enzymatic antioxidants (SOD, GR, GPx, GST, CAT, GSH, and TAC) as well as renal (urea, creatinine) and hepatic (ALT, AST) functions, induced antiproliferative genes (caspase 3,9, p53, Bax, TNFalpha), suppressed antiapoptotic and antiangiogenic genes (Bcl2,VEGF respectively) and effectively reducing the growth of tumors and even leading to cancer cell death. This process could be attributed to photochemical and/or high-frequency sono-chemical activation mechanism HIFU–SPDT. Conclusions The results indicate that CCF–HIONP has great promise as an innovative, effective delivery system for selective localized treatment of lung cancer that is activated by HIFU–SPDT.

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Anti-Cancer Properties of Flaxseed Proteome

Cited by 3 publications

References 164 publications

Proteomic Profile of Flaxseed (Linum usitatissimum L.) Products as Influenced by Protein Concentration Method and Cultivar

Proteomic Profile of Flaxseed (Linum usitatissimum L.) Products as Influenced by Protein Concentration Method and Cultivar

Therapeutic implications of flaxseed peptides and bioactive components against various diseases

High-frequency ultrasound-assisted drug delivery of chia, cress, and flax conjugated hematite iron oxide nanoparticle for sono-photodynamic lung cancer treatment in vitro and in vivo

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