Anti-cancer Effects of Azadirachta indica in Diethylnitrosamine-Induced Hepatocellular Carcinoma in Wistar Rats

Abstract

 Abstract: Hepatocellular carcinoma (HCC) is a primary liver cancer and has a high rate of mortality. Exploration of local herbal as a chemo-preventive drug has been conducted. This present study aimed to observe the anti-cancer effect of hydroethanolic extracts of Azadirachta indica both Indonesia (HEAII) and the Philippines (HEAIP) on diethylnitrosamine (DEN)-induced HCC on Wistar rat model. VEGF/FGF2/CD166/YAP were evaluated using immunohistochemistry. VEGF/FGF2/CD166/YAP were down-regulated in HCC treated with HEAII and HEAIP compare to that non-treatment HCC. Liver function (aminotransferase (AST) and aminotransferase (ALT) activities) was evaluated from serum. Value of AST and ALT were abnormal in the non-treatment HCC and normal in the HCC treated. These results indicated that both extracts were found to possess anti-cancer activity in HCC.Keywords: hepatocelullar carcinoma; Azadirachta indica; cluster differential 166 (CD166); fibroblast growth factor 2(FGF-2); yes associated protein (YAP); vascular endothelial growth factor (VEGF)