Anti-cancer Effect of Luminacin, a Marine Microbial Extract, in Head and Neck Squamous Cell Carcinoma Progression via Autophagic Cell Death

Shin, Yoo Seob; Young, Hyun; Lee, Bok‐Soon; Kang, Sung Un; Hwang, Hye Sook; Kwon, Hak Cheol; Kim, Chul‐Ho; Choi, Eun Chang

doi:10.4143/crt.2015.102

Cited by 22 publications

(21 citation statements)

References 25 publications

(27 reference statements)

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“…Immunohistochemistry was performed on paraffin-embedded of HNC tissues or in vivo xenograft tumor sections [54]. Briefly, the specimens were incubated in blocking solution with the indicated antibodies overnight at 4 C. The sections were thoroughly rinsed in PBS and incubated for 2 h at room temperature with the SPlink HRP Detection Kit (Golden Bridge International Labs, D03-110).…”

Section: Immunohistochemistrymentioning

confidence: 99%

HSPA5 negatively regulates lysosomal activity through ubiquitination of MUL1 in head and neck cancer

Kim

et al. 2018

Autophagy

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HSPA5/GRP78/BiP plays an important role in cell survival or tumor progression. For these reasons, HSPA5 is an emerging therapeutic target in cancer development. Here we report that HSPA5 contributes to head and neck cancer (HNC) survival via maintenance of lysosomal activity; however, a nonthermal plasma (NTP, considered as a next-generation cancer therapy)-treated solution (NTS) inhibits HNC progression through HSPA5-dependent alteration of lysosomal activity. HSPA5 prevents NTS-induced lysosome inhibition through lysosomal-related proteins or regulation of gene expression. However, NTS-induced MUL1/MULAN/GIDE/MAPL (mitochondrial ubiquitin ligase activator of NFKB 1) leads to downregulation of HSPA5 via K48-linked ubiquitination at the lysine 446 (K446) residue. MUL1 knockdown hinders NTS-induced lysosome inhibition or cytotoxicity through the reduction of HSPA5 ubiquitination in HNC cells. While MUL1 was suppressed, HSPA5 was overexpressed in tissues of HNC patients. NTS strongly inhibited HNC progression via alterations of expression of MUL1 and HSPA5, in vivo in a xenograft model. However, NTS did not induce inhibition of tumor progression or HSPA5 reduction in MUL1 knockout (KO) HNC cells which were generated by CRISPR/Cas9 system. The data provide compelling evidence to support the idea that the regulation of the MUL1-HSPA5 axis can be a novel strategy for the treatment of HNC.

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Section: Immunohistochemistrymentioning

confidence: 99%

HSPA5 negatively regulates lysosomal activity through ubiquitination of MUL1 in head and neck cancer

Kim

et al. 2018

Autophagy

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“…Skin cancer, which includes melanoma and squamous cell carcinoma (SCC), represents the most common type of cutaneous malignancy worldwide, and its incidence is expected to rise in the near future [ 21 ]. Melanoma is the deadliest form of skin cancer, with respect to all skin cancer-related deaths, and has a mortality rate of 80% [ 22 , 23 ]. Melanoma arises from melanocytes, the pigmented epidermal cells responsible for the production of melanin.…”

Section: Introductionmentioning

confidence: 99%

Zebrafish as a Model Organism for the Development of Drugs for Skin Cancer

Bootorabi

Manouchehri

Changizi

et al. 2017

IJMS

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Skin cancer, which includes melanoma and squamous cell carcinoma, represents the most common type of cutaneous malignancy worldwide, and its incidence is expected to rise in the near future. This condition derives from acquired genetic dysregulation of signaling pathways involved in the proliferation and apoptosis of skin cells. The development of animal models has allowed a better understanding of these pathomechanisms, with the possibility of carrying out toxicological screening and drug development. In particular, the zebrafish (Danio rerio) has been established as one of the most important model organisms for cancer research. This model is particularly suitable for live cell imaging and high-throughput drug screening in a large-scale fashion. Thanks to the recent advances in genome editing, such as the clustered regularly-interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) methodologies, the mechanisms associated with cancer development and progression, as well as drug resistance can be investigated and comprehended. With these unique tools, the zebrafish represents a powerful platform for skin cancer research in the development of target therapies. Here, we will review the advantages of using the zebrafish model for drug discovery and toxicological and phenotypical screening. We will focus in detail on the most recent progress in the field of zebrafish model generation for the study of melanoma and squamous cell carcinoma (SCC), including cancer cell injection and transgenic animal development. Moreover, we will report the latest compounds and small molecules under investigation in melanoma zebrafish models.

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“…Skin or dermal cancers represent the most common type of cutaneous malignancy globally, which includes melanoma and carcinoma of squamous cells [70]. Melanoma is the most pernicious form of skin cancer among all types of skin cancers and has mortality rate over 80% [71,72]. Melanoma usually develops in the pigmented epidermal cells (melanocytes), which are responsible for the production of melanin.…”

Section: Skin Cancermentioning

confidence: 99%

“…The zebrafish can be used to study melanoma development, progression, drug screening, and treatment. The zebrafish model has been exploited recently to recognize the key molecules which are responsible for the development of cutaneous squamous cell carcinoma (cSCC) and head and neck squamous cell carcinoma (HNSCC) [72] as well as for SCC target therapies [79].…”

Section: Skin Cancermentioning

confidence: 99%

Zebrafish (Danio rerio) as a Model Organism

Khan¹,

Alhewairini²

2019

Current Trends in Cancer Management

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Animals as model organisms, the silent sentinels, stand watch over the environmental health of the world. These are non-human animal species which can be used to understand specific biological processes and to obtain informations which can provide an insight into working of other organisms. Among the model organisms, the zebrafish (Danio rerio) is one of the best leading models to study developmental biology, cancer, toxicology, drug discovery, and molecular genetics. In addition, the zebrafish is increasingly used as a genetic model organism for aquaculture species and in toxicogenomics and also to generate zebrafish disease models for application in human biomedicines. This tiny fish is a versatile model organism for many fields of research because of its easy maintenance, breeding, and transparent body during early development.

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Anti-cancer Effect of Luminacin, a Marine Microbial Extract, in Head and Neck Squamous Cell Carcinoma Progression via Autophagic Cell Death

Cited by 22 publications

References 25 publications

HSPA5 negatively regulates lysosomal activity through ubiquitination of MUL1 in head and neck cancer

HSPA5 negatively regulates lysosomal activity through ubiquitination of MUL1 in head and neck cancer

Zebrafish as a Model Organism for the Development of Drugs for Skin Cancer

Zebrafish (Danio rerio) as a Model Organism

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