Anti-cancer effect of (-)-epigallocatechin-3-gallate (EGCG) in head and neck cancer through repression of transactivation and enhanced degradation of β-catenin

Shin, Yoo Seob; Kang, Sung Un; Park, Ju Kyeong; Kim, Yang Eun; Kim, Yeon Soo; Baek, Seung Joon; Lee, Seong-Ho; Kim, Chul‐Ho

doi:10.1016/j.phymed.2016.07.005

Cited by 41 publications

(18 citation statements)

References 25 publications

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“…In the present study, EGCG was demonstrated to suppress the viability of esophageal cancer Eca109 and Ec9706 cells via inducing apoptosis in an EGCG dose-dependent manner. EGCG has been reported to induce cancer cell apoptosis through different pathways involving the pro-oxidant, epigenetic modulation of apoptosis-related genes, including human telomerase reverse transcriptase, and to reduce cell proliferation through the modulation of cell cycle progression (25)(26)(27).…”

Section: Discussionmentioning

confidence: 99%

“…EGCG is the ester form of epigallocatechin/gallic acid; it is the main catechin in green tea and contributes to its beneficial therapeutic effects, which include antioxidant and immunomodulatory effects (7)(8)(9)(10). Due to its reported anti-oxidant and immunomodulatory effects, EGCG has been extensively investigated against various types of cancer (11)(12)(13). EGCG has not been observed to cause adverse effects against normal cells and tissues, whereas it has anti-proliferative, anti-invasive and chemo-preventive effects against cancer cells (14).…”

Section: Introductionmentioning

confidence: 99%

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Epigallocatechin-3-gallate suppresses cell proliferation and promotes apoptosis in Ec9706 and Eca109 esophageal carcinoma cells

2017

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Abstract. Epigallocatechin-3-gallate (EGCG) is a component of green tea with anticancer effects that have been demonstrated in multiple types of cancer, but few reports exist concerning its effect in esophageal squamous cell carcinoma cells. The present study investigated apoptosis induced by EGCG treatment and the underlying molecular mechanisms in Eca109 and Ec9706 human esophageal squamous cell carcinoma cells. The apoptosis rate following treatment with various concentration of EGCG for 24 h was detected by flow cytometry. The effect of EGCG on esophageal cancer cell viability was detected via MTT assay. Mitochondrial membrane potential and caspase-3 protein expression was detected in Eca109 and Ec9706 cells following treatment with EGCG by flow cytometry. The telomerase activity of Eca109 and Ec9706 cells following treatment with EGCG was assayed using the polymerase chain reaction-telomeric repeat amplification protocol (PCR-TRAP) argentation method. EGCG was demonstrated to inhibit the viability of Eca109 and Ec9706 cells in a dose-and time-dependent manner. The flow cytometry results revealed that EGCG treatment induced apoptosis, decreased the mitochondrial membrane potential and increased caspase-3 protein expression levels. PCR-TRAP argentation analysis revealed that EGCG inhibited telomerase activity. The results of the present study suggested that EGCG functions as an antitumor agent in esophageal cancer cells. The induction of apoptosis may be a viable method for treating esophageal cancer. It is possible to induce apoptosis by modulating the expression level of telomerase activity, mitochondrial membrane potential and caspase-3 protein expression levels.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Epigallocatechin-3-gallate suppresses cell proliferation and promotes apoptosis in Ec9706 and Eca109 esophageal carcinoma cells

2017

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“…It inhibits cell proliferation in human bladder cancer SW-780, breast cancer MDA-MB-231 and NSCLC A549 cells, and inhibits tumor growth in gastric cancer SGC-7901 xenograft mice [89,94,95]. It also induces apoptosis in human oral cancer KB, head and neck cancer FaDu, NSCLC A549, and breast cancer MCF-7 cells [96,97]. Besides, EGCG induces autophagy, and inhibition of autophagy can enhance EGCG-induced cell death in human mesothelimoa ACC-meso, Y-meso, EHMES-10, EHMES-1 and MSTO-211H, and primary effusion lymphoma BCBL-1 and BC-1 cells [98,99].…”

Section: Epigallocatechin Gallate (Egcg)mentioning

confidence: 99%

Naturally occurring anti-cancer compounds: shining from Chinese herbal medicine

et al. 2019

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Numerous natural products originated from Chinese herbal medicine exhibit anti-cancer activities, including antiproliferative, pro-apoptotic, anti-metastatic, anti-angiogenic effects, as well as regulate autophagy, reverse multidrug resistance, balance immunity, and enhance chemotherapy in vitro and in vivo. To provide new insights into the critical path ahead, we systemically reviewed the most recent advances (reported since 2011) on the key compounds with anti-cancer effects derived from Chinese herbal medicine (curcumin, epigallocatechin gallate, berberine, artemisinin, ginsenoside Rg3, ursolic acid, silibinin, emodin, triptolide, cucurbitacin B, tanshinone I, oridonin, shikonin, gambogic acid, artesunate, wogonin, β-elemene, and cepharanthine) in scientific databases (PubMed, Web of Science, Medline, Scopus, and Clinical Trials). With a broader perspective, we focused on their recently discovered and/or investigated pharmacological effects, novel mechanism of action, relevant clinical studies, and their innovative applications in combined therapy and immunomodulation. In addition, the present review has extended to describe other promising compounds including dihydroartemisinin, ginsenoside Rh2, compound K, cucurbitacins D, E, I, tanshinone IIA and cryptotanshinone in view of their potentials in cancer therapy. Up to now, the evidence about the immunomodulatory effects and clinical trials of natural anti-cancer compounds from Chinese herbal medicine is very limited, and further research is needed to monitor their immunoregulatory effects and explore their mechanisms of action as modulators of immune checkpoints.

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“…EGCG has revealed several beneficial health effects, including anti-inflammatory (Cavet et al 2011), anti-carcinogenic (Farabegoli et al 2011;Santos et al 2013;Radhakrishnan et al 2016;Shin et al 2016), antioxidant (Cavet et al 2011;Zhou and Elias 2013), antiangiogenic (Yamakawa et al 2004;Piyaviriyakul et al 2011), anti-diabetic (Wolfram et al 2006;Chen N et al 2009) and anti-bacterial (Lee S et al 2017). It has also been reported its use as chondroprotective agent as it suppressed the inflammatory response in osteoarthritis models (Akhtar and Haqqi 2011;Min S-Y et al 2015), as well as a cardiovascular protector (Wolfram 2007;Oyama et al 2017) and neuroprotector (Lee JH et al 2015;Ortiz-López et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Comparison of antiproliferative effect of epigallocatechin gallate when loaded into cationic solid lipid nanoparticles against different cell lines

Silva

Martins-Gomes

Fangueiro

et al. 2019

Pharmaceutical Development and Technology

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Several therapeutic properties have been attributed to epigallocatechin gallate (EGCG), a phytopharmaceutical polyphenol with antioxidant and antiproliferative activity.EGCG is however very prone to oxidation in aqueous solutions which changes its bioactive properties. Its loading in nanoparticles has been proposed to reduce its degradation while increasing its in vivo efficacy. The aim of this study was to compare the antiproliferative effect of EGCG before and after its loading in solid lipid nanoparticles (SLNs), against five different cell lines (Caco-2, HepG2, MCF-7, SV-80 and Y-79). EGCG produced concentration-and time-dependent antiproliferative effect, with eficacy dependent on the cell line. The order of potency was: MCF-7>SV-80>HepG2>Y-79>Caco-2, for 24h exposure (MCF-7 IC 50 =58.60±3.29 µg/mL; Caco-2 IC 50 >500.00 µg/mL). To the best of our knowledge this is the first study reporting EGCG antiproliferative effect in SV-80 and Y-79 cells. DDAB-SLN physicochemical properties (size 134nm; PI0.179; ZP +28mV) were only slightly modified with EGCG loading (EGCG-DDAB-SLN: 144nm; PI0.160; ZP +26mV). EGCG loadingin SLN, only slightly increases the EGCG antiproliferative effect in MCF-7 and SV-80 cells. SLN exhibited intrinsic toxicity, attributed to the surfactant used in its production. From the obtained results, the biocompatibility of blank SLN must be also considered when testing the efficacy of loaded phytopharmaceutics.

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Anti-cancer effect of (-)-epigallocatechin-3-gallate (EGCG) in head and neck cancer through repression of transactivation and enhanced degradation of β-catenin

Cited by 41 publications

References 25 publications

Epigallocatechin-3-gallate suppresses cell proliferation and promotes apoptosis in Ec9706 and Eca109 esophageal carcinoma cells

Epigallocatechin-3-gallate suppresses cell proliferation and promotes apoptosis in Ec9706 and Eca109 esophageal carcinoma cells

Naturally occurring anti-cancer compounds: shining from Chinese herbal medicine

Comparison of antiproliferative effect of epigallocatechin gallate when loaded into cationic solid lipid nanoparticles against different cell lines

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