Anti-Breast Cancer Activity on MCF-7 Cells of Melittin from Indonesia’s Apis cerana: An In Vitro Study

Lischer, Kenny; Sitorus, Shania Rosita Angelica; Guslianto, Brian Wirawan; Avila, Forbes; Khayrani, Apriliana Cahya; Sahlan, Muhamad

doi:10.31557/apjcp.2021.22.12.3913

Cited by 9 publications

(4 citation statements)

References 54 publications

(64 reference statements)

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“…In what concerns the MCF-7 cell line, the bee venom-loaded niosomes presented a GI 50 value of 14.05 ng/mL, thereby being substantially cytotoxic for this cell line as well. Bee venom has been proven to inhibit the growth of breast cancer cells even in small concentrations, as observed in previous studies [61]. For the NCIH460 cell line, the bee venom-loaded niosomes showed a GI 50 value of 14.72 ng/mL, hence demonstrating good anticancer potential for this cell line too.…”

Section: In Vitro Cytotoxic Potentialsupporting

confidence: 71%

Bee Venom-Loaded Niosomes as Innovative Platforms for Cancer Treatment: Development and Therapeutical Efficacy and Safety Evaluation

Pinto,

Pires,

Calhelha

et al. 2024

Pharmaceuticals

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Despite past efforts towards therapeutical innovation, cancer remains a highly incident and lethal disease, with current treatments lacking efficiency and leading to severe side effects. Hence, it is imperative to develop new, more efficient, and safer therapies. Bee venom has proven to have multiple and synergistic bioactivities, including antitumor effects. Nevertheless, some toxic effects have been associated with its administration. To tackle these issues, in this work, bee venom-loaded niosomes were developed, for cancer treatment. The vesicles had a small (150 nm) and homogeneous (polydispersity index of 0.162) particle size, and revealed good therapeutic efficacy in in vitro gastric, colorectal, breast, lung, and cervical cancer models (inhibitory concentrations between 12.37 ng/mL and 14.72 ng/mL). Additionally, they also revealed substantial anti-inflammatory activity (inhibitory concentration of 28.98 ng/mL), effects complementary to direct antitumor activity. Niosome safety was also assessed, both in vitro (skin, liver, and kidney cells) and ex vivo (hen’s egg chorioallantoic membrane), and results showed that compound encapsulation increased its safety. Hence, small, and homogeneous bee venom-loaded niosomes were successfully developed, with substantial anticancer and anti-inflammatory effects, making them potentially promising primary or adjuvant cancer therapies. Future research should focus on evaluating the potential of the developed platform in in vivo models.

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Section: In Vitro Cytotoxic Potentialsupporting

confidence: 71%

Bee Venom-Loaded Niosomes as Innovative Platforms for Cancer Treatment: Development and Therapeutical Efficacy and Safety Evaluation

Pinto,

Pires,

Calhelha

et al. 2024

Pharmaceuticals

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“…In addition, it is cell nonspecific and exhibits hemolytic properties, which are a challenge for the use of this peptide in medicine [49]. Researchers tested MEL for its anticancer properties against a number of cancers, including breast cancer [50][51][52]. The use of nanoliposomes and MEL encapsulation with polomaxer 188 has been shown to control hepatocellular carcinoma tumors without serious side effects [53].…”

Section: Discussionmentioning

confidence: 99%

Delivery of Melittin as a Lytic Agent via Graphene Nanoparticles as Carriers to Breast Cancer Cells

Daniluk

Lange

Pruchniewski

et al. 2022

JFB

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Melittin, as an agent to lyse biological membranes, may be a promising therapeutic agent in the treatment of cancer. However, because of its nonspecific actions, there is a need to use a delivery method. The conducted research determined whether carbon nanoparticles, such as graphene and graphene oxide, could be carriers for melittin to breast cancer cells. The studies included the analysis of intracellular pH, the potential of cell membranes, the type of cellular transport, and the expression of receptor proteins. By measuring the particle size, zeta potential, and FT-IT analysis, we found that the investigated nanoparticles are connected by electrostatic interactions. The level of melittin encapsulation with graphene was 86%, while with graphene oxide it was 78%. A decrease in pHi was observed for all cell lines after administration of melittin and its complex with graphene. The decrease in membrane polarization was demonstrated for all lines treated with melittin and its complex with graphene and after exposure to the complex of melittin with graphene oxide for the MDA-MB-231 and HFFF2 lines. The results showed that the investigated melittin complexes and the melittin itself act differently on different cell lines (MDA-MB-231 and MCF-7). It has been shown that in MDA-MD-231 cells, melittin in a complex with graphene is transported to cells via caveolin-dependent endocytosis. On the other hand, the melittin–graphene oxide complex can reach breast cancer cells through various types of transport. Other differences in protein expression changes were also observed for tumor lines after exposure to melittin and complexes.

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“…The use of natural products and their active ingredients in the prevention and treatment of chronic diseases is largely based on the experience of the traditional medical system found in different ethnic communities and epidemiological data of the relationship between diet and disease [ 12 , 21 , 35 , 36 ]. Although Apis mellifera venom has been studied the most extensively, there are several other Apis species, namely, Apis cerana and Apis florea , whose venom and/or peptides have also shown promising pharmacological effects and anticancer properties [ 43 , 44 ].…”

Section: Introductionmentioning

confidence: 99%

Bee Venom: Composition and Anticancer Properties

Gajski,

Leonova,

Sjakste

2024

Toxins

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Among the various natural compounds used in alternative and Oriental medicine, toxins isolated from different organisms have had their application for many years, and Apis mellifera venom has been studied the most extensively. Numerous studies dealing with the positive assets of bee venom (BV) indicated its beneficial properties. The usage of bee products to prevent the occurrence of diseases and for their treatment is often referred to as apitherapy and is based mainly on the experience of the traditional system of medical practice in diverse ethnic communities. Today, a large number of studies are focused on the antitumor effects of BV, which are mainly attributed to its basic polypeptide melittin (MEL). Previous studies have indicated that BV and its major constituent MEL cause a strong toxic effect on different cancer cells, such as liver, lung, bladder, kidney, prostate, breast, and leukemia cells, while a less pronounced effect was observed in normal non-target cells. Their proposed mechanisms of action, such as the effect on proliferation and growth inhibition, cell cycle alterations, and induction of cell death through several cancer cell death mechanisms, are associated with the activation of phospholipase A2 (PLA2), caspases, and matrix metalloproteinases that destroy cancer cells. Numerous cellular effects of BV and MEL need to be elucidated on the molecular level, while the key issue has to do with the trigger of the apoptotic cascade. Apoptosis could be either a consequence of the plasmatic membrane fenestration or the result of the direct interaction of the BV components with pro-apoptotic and anti-apoptotic factors. The interaction of BV peptides and enzymes with the plasma membrane is a crucial step in the whole process. However, before its possible application as a remedy, it is crucial to identify the correct route of exposure and dosage of BV and MEL for potential therapeutic use as well as potential side effects on normal cells and tissues to avoid any possible adverse event.

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Anti-Breast Cancer Activity on MCF-7 Cells of Melittin from Indonesia’s Apis cerana: An In Vitro Study

Cited by 9 publications

References 54 publications

Bee Venom-Loaded Niosomes as Innovative Platforms for Cancer Treatment: Development and Therapeutical Efficacy and Safety Evaluation

Bee Venom-Loaded Niosomes as Innovative Platforms for Cancer Treatment: Development and Therapeutical Efficacy and Safety Evaluation

Delivery of Melittin as a Lytic Agent via Graphene Nanoparticles as Carriers to Breast Cancer Cells

Bee Venom: Composition and Anticancer Properties

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