Anti-biofouling properties of an amphoteric polymer brush constructed on a glass substrate

Kitano, Hiromi; Kondo, Takuya; Kamada, Tomohiro; Iwanaga, Shintaroh; Nakamura, Makoto; Ohno, Kohji

doi:10.1016/j.colsurfb.2011.07.029

Cited by 47 publications

(36 citation statements)

References 57 publications

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“…Zwitterionic polymers, which have both anion and cation in a single molecule, are known as excellent bio-functional materials that prevent both cell adhesion and protein adsorption [17,18] , and are also useful for bio-patterning [19,20] . In this work, zwitterionic polymers were prepared using 1-carboxy-N,Ndimethyl-N-(2'-methacryloyloxyethyl)methanaminium inner salt also known as carboxymethyl betaine (CMB) was kindly donated from Osaka Organic Chemical Industry, Kashiwara, Japan, and n-butylmethacrylate (BMA) obtained from Wako, Osaka, Japan, at the rate of (CMB/BMA = 29/71; MW = 193 kDa and MW/Mn =1.92; 1 wt% ethanol solution), and utilized as a surface patterning material.…”

Section: Preparation Of Surface Patterned Discs With Non-cell-adherenmentioning

confidence: 99%

Bioprinting with pre-cultured cellular constructs towards tissue engineering of hierarchical tissues

Nakamura¹,

Mir²,

Arai³

et al. 2024

IJB

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The fabrication of physiologically active tissue constructs from various tissue elements are vital for establishing integrated bioprinting and transfer printing techniques as vital tools for biomedical research. Physiologically functional tissues are hierarchically constructed from a variety of tissue subunits with different feature sizes and topographies. For example, skeletal muscles are composed of many muscle bundles, muscle fibers, and muscle cells respectively. The fundamental constituents of all types of muscle tissues include various sized blood vessels, and vascular related cells. Nature has designed the direction of all the aforementioned components to have unidirectional alignment, so that muscle contractions can effectively generate the mechanical functions. In this study, we demonstrate a promising approach to fabricating such hierarchical tissues by applying bioprinting and a transfer patterning technique. Linear-patterned smooth muscle cells were obtained by culturing on the surface patterned discs, before being transferred onto the Matrigel substrate. The fiber-like tissues structures were successfully formed on the substrate after a few days of culturing; these are partially aligned smooth muscle cells. Additionally, stacked structures were also successfully fabricated using laminating printing technique. Our results indicate that bioprinting and transfer printing strategy of pre-cultured aligned muscular fiber-like tissues is very promising method to assemble tissue elements for biofabrication of hierarchical tissues.

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Section: Preparation Of Surface Patterned Discs With Non-cell-adherenmentioning

confidence: 99%

Bioprinting with pre-cultured cellular constructs towards tissue engineering of hierarchical tissues

Nakamura¹,

Mir²,

Arai³

et al. 2024

IJB

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“…One interesting feature of zwitterionic polymers is its anti-biofouling property2526. This is due to the presence of a nanoscale homogenous mixture of both the charges located close to each other27.…”

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confidence: 99%

Inhibition of protein aggregation by zwitterionic polymer-based core-shell nanogels

Rajan

Matsumura

2017

Sci Rep

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Protein aggregation is a process by which misfolded proteins polymerizes into aggregates and forms fibrous structures with a β-sheet conformation, known as amyloids. It is an undesired outcome, as it not only causes numerous neurodegenerative diseases, but is also a major deterrent in the development of protein biopharmaceuticals. Here, we report a rational design for the synthesis of novel zwitterionic polymer-based core-shell nanogels via controlled radical polymerization. Nanogels with different sizes and functionalities in the core and shell were prepared. The nanogels exhibit remarkable efficiency in the protection of lysozyme against aggregation. Addition of nanogels suppresses the formation of toxic fibrils and also enables lysozyme to retain its enzymatic activity. Increasing the molecular weight and degree of hydrophobicity markedly increases its overall efficiency. Investigation of higher order structures revealed that lysozyme when heated without any additive loses its secondary structure and transforms into a random coil conformation. In contrast, presence of nanogels facilitates the retention of higher order structures by acting as molecular chaperones, thereby reducing molecular collisions. The present study is the first to show that it is possible to design zwitterionic nanogels using appropriate polymerization techniques that will protect proteins under conditions of extreme stress and inhibit aggregation.

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“…BSA was negatively charged, whereas lysozyme was positively charged in the HBS used for measurement. Nonspecific absorption is caused by electrostatic or hydrophobic interaction; it is evaluated using BSA and lysozyme [18].…”

Section: Evaluation Of Nonspecific Adsorptionmentioning

confidence: 99%

Preparation and Characteristics of Rat Anti-1,3,5-Trinitroperhydro-1,3,5-Triazine (RDX) Monoclonal Antibody and Detection of RDX Using Surface Plasmon Resonance Immunosensor

et al. 2013

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In this paper, we develop a method of detecting 1,3,5-trinitroperhydro-1,3,5-triazine [research department explosive (RDX)]. RDX is one of the main components of plastic explosives. Initially, we prepare an anti-RDX monoclonal antibody that specifically binds to RDX. The antibody is prepared from hybridoma cells that are prepared by the iliac lymph node method. The dissociation constant of the antibody against RDX is estimated to be 9.6 nM from the results of indirect competitive enzyme-linked immunosorbent assay. We fabricate a sensor chip on which RDX analogues are immobilized through a self-assembled monolayer with oligo(ethylene glycol) chains. High reliability and low false recognition rate are particularly required for sensors used for detecting explosives. Therefore, the nonspecific adsorption of the fabricated sensor chip is evaluated to confirm whether the sensor chip inhibits this nonspecific adsorption. The response characteristics of the prepared antibody against RDX are examined by indirect competitive assay. Under an incubation time of 0 min in the indirect competitive assay, a detection limit of 40 ppt is realized at a sample injection duration of 6 min.

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Anti-biofouling properties of an amphoteric polymer brush constructed on a glass substrate

Cited by 47 publications

References 57 publications

Bioprinting with pre-cultured cellular constructs towards tissue engineering of hierarchical tissues

Bioprinting with pre-cultured cellular constructs towards tissue engineering of hierarchical tissues

Inhibition of protein aggregation by zwitterionic polymer-based core-shell nanogels

Preparation and Characteristics of Rat Anti-1,3,5-Trinitroperhydro-1,3,5-Triazine (RDX) Monoclonal Antibody and Detection of RDX Using Surface Plasmon Resonance Immunosensor

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