Anti‐biofilm Effects of Honey Against Wound Pathogens <i>Proteus mirabilis</i> and <i>Enterobacter cloacae</i>

Majtán, Juraj; Bohova, Jana; Horniackova, Miroslava; Klaudiny, Jaroslav; Majtán, V.

doi:10.1002/ptr.4957

Cited by 90 publications

(64 citation statements)

References 28 publications

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“…The plates were incubated in an orbital shaker (1500 r.p.m.) at 37 uC for 24 and 48 h. After incubation, the biofilm formation was measured using a crystal violet staining method according to our previous study to detect polymeric substances (Majtan et al, 2014). The viability of biofilm-forming cells was measured using resazurin according to van den Driessche et al (2014).…”

Section: Pcrmentioning

confidence: 99%

“…A few studies have attempted to identify the active compounds responsible for the antibiofilm activity of honey (Truchado et al, 2009;Lee et al, 2011;Majtan et al, 2014). Most of these compounds take part in the prevention of biofilm formation, but little information is available regarding compounds that are able to disrupt established wound biofilm.…”

Section: Introductionmentioning

confidence: 99%

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Antibiofilm efficacy of honey and bee-derived defensin-1 on multispecies wound biofilm

Sojka

Valachová

Bucekova

et al. 2016

Journal of Medical Microbiology

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Many clinically relevant biofilms are polymicrobial. Examining the effect of antimicrobials in a multispecies biofilm consortium is of great clinical importance. The goal of this study was to investigate the effect of different honey types against bacterial wound pathogens grown in multispecies biofilm and to test the antibiofilm activity of honey defensin-1 (Def-1) in its recombinant form. A modified Lubbock chronic wound biofilm formed by four bacterial species (Staphylococcus aureus, Streptococcus agalactiae, Pseudomonas aeruginosa and Enterococcus faecalis) was used for evaluation of honey and recombinant bee-derived Def-1 antibiofilm efficacy. Recombinant Def-1 was prepared by heterologous expression in Escherichia coli. We showed that different types of honey (manuka and honeydew) were able to significantly reduce the cell viability of wound pathogens (Staphylococcus aureus, Streptococcus agalactiae and Pseudomonas aeruginosa) in mature polymicrobial biofilm. None of the tested honeys showed the ability to eradicate Enterococcus faecalis in biofilm. In addition, recombinant Def-1 successfully reduced the viability of Staphylococcus aureus and Pseudomonas aeruginosa cells within established polymicrobial biofilm after 24 and 48 h of treatment. Interestingly, recombinant Def-1 did not affect the viability of Streptococcus agalactiae cells within the biofilm, whereas both natural honeys significantly reduced the viable bacteria. Although Enterococcus faecalis was highly resistant to Def-1, Def-1 significantly affected the biofilm formation of Enterococcus faecalis and Streptococcus agalactiae after 24 h of treatment, most likely by inhibiting its extracellular polymeric substances production. In conclusion, our study revealed that honey and Def-1 are effective against established multispecies biofilm; however, Enterococcus faecalis grown in multispecies biofilm was resistant to both antimicrobials.

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Section: Pcrmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antibiofilm efficacy of honey and bee-derived defensin-1 on multispecies wound biofilm

Sojka

Valachová

Bucekova

et al. 2016

Journal of Medical Microbiology

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“…The anti-biofilm property of the honey was attributed to methylglyoxal. 38 Currently, anti-biotic use poses a well established impediment, due to the emergence of resistant bacteria. On the other hand, due to multi-phytochemical composition and the array of differing anti-microbial mechanisms, manuka honey seems well presented to inhibit even antibiotic-resistant 'superbugs'.…”

Section: Anti-bacterial Anti-biotic and Anti-viral Effectsmentioning

confidence: 99%

Manuka honey: an emerging natural food with medicinal use

Patel

Cichello

2013

Nat. Prod. Bioprospect.

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Abstract:The health value of honey is universally acknowledged from time immemorial. Manuka (Leptospermum scoparium) is a tree, indigenous to New Zealand and South East Australia, and from the myrtle family, Myrtaceae. The honey produced from its flowers is a uni-floral honey largely produced in New Zealand. It is becoming increasingly popular as a functional food, seen in the aisles of health stores as its displays superior nutritional and phytochemistry profile over other varieties of honey. Examining existing research databases revealed its biological properties ranging from anti-oxidant, anti-inflammatory, anti-bacterial, anti-viral, anti-biotic and wound healing to immune-stimulatory properties. Methylglyoxal is the unique compound in the honey responsible for some of its potent anti-microbial properties. Further, propolis another component of honey contains chiefly flavonoids (i.e. galangin, pinocembrin), phenolic acids and their esters that may also contribute to its immuno-stimulant properties. Recent findings of the biological roles have been discussed with emphasis on the underlying mechanisms. The hurdles associated in its development as a functional food and also nutraceutical with future scopes have also been mentioned. Relevant data published in MEDLINE, Cochrane library, and EMBASE in the past decade have been gathered to formulate this review.

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“…Similar to our finding, Alandejani et al [47] by using Sidr and Manuka honeys reported that biofilm-producing strains of P. aeruginosa were more susceptible to inhibitory effect of honey samples than S. aureus strains. According to study of Majtan et al [48], it has been suggested that methylglyoxal, an antibacterial compound of Manuka honey, is responsible for killing biofilm-embedded wound bacteria. However, further studies are necessary to determine the mechanism by which honey prevents biofilm formation.…”

Section: Discussionmentioning

confidence: 99%

In vitro characterization of antibacterial potential of Iranian honey samples against wound bacteria

Mahmoodi-Khaledi

Kashef

Habibi-Rezaei

et al. 2015

Eur Food Res Technol

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ranged from 3.12 to >50 % (w/v), and their efficacies were also determined. Nine samples with the highest antibacterial activities were selected. Survival of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa was reduced significantly by selected honeys. Moreover, all selected honey samples were able to prevent biofilm formation of S. aureus and P. aeruginosa strains at concentrations of ≤25 %. The results of the present study have shown that selected Iranian honey samples had lower MIC values than reported standard honeys such as Manuka and Tualang and could be potentially used as alternative medical grade honey, particularly against S. aureus, P. aeruginosa, and E. coli.

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Anti‐biofilm Effects of Honey Against Wound Pathogens Proteus mirabilis and Enterobacter cloacae

Cited by 90 publications

References 28 publications

Antibiofilm efficacy of honey and bee-derived defensin-1 on multispecies wound biofilm

Antibiofilm efficacy of honey and bee-derived defensin-1 on multispecies wound biofilm

Manuka honey: an emerging natural food with medicinal use

In vitro characterization of antibacterial potential of Iranian honey samples against wound bacteria

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