Anti-Biofilm, Anti-Quorum Sensing, and Anti-Proliferative Activities of Methanolic and Aqueous Roots Extracts of <i>Carica papaya</i> L. and <i>Cocos nucifera</i> L.

Abstract

Objective: This study focused on the antibacterial and anti-proliferative activity of extracts from Carica papaya and Cocos nucifera roots. Methodology:The minimum inhibitory concentration and the minimum bactericidal concentration of the extracts on Escherichia coli, Pseudomonas aeruginosa, Streptococcus mutans, and Staphylococcus aureus were deduced by the microdilution method. The anti-biofilm activity was determined on all four strains and anti-quorum sensing activity by inhibition of violacein production in Chromobacterium violaceum. Anti-proliferative activity on prostate cultured cancer cells was evaluated by MTT assay. Sterols and triterpenes were also assayed in this study. Results: The methanolic extract of Carica papaya showed the best anti-biofilm effect with a percentage inhibition of 66.10 ± 1.79. The methanolic extract of Cocos nucifera had the strongest inhibition on the production of quorum sensing (61.42 ± 0.28). In addition, the methanolic extract of Cocos nucifera roots showed the best cytotoxic effect on prostate cancer LNCaP cell lines (IC 50 = 26.98 ± 2.6 μg/mL) and Carica papaya on the PC-3 lines (IC 50 = 127.20 ± 5.99 μg/mL). The extracts were also rich in triterpenes and sterols. Conclusion: This study provides support for How to cite this paper: Belem-Kabré,

Anti-quorum Sensing of Terminalia catappa and Murraya koenigii Against Streptococcus mutans

“…Anti-biofilm activity of certain medicinal plants against S. mutans has been studied previously. Belem-Kabré et al reported that at a concentration of 100 μg/mL, the methanolic extract of Carica papaya suppressed 66.10% of the biofilm produced by S. mutans [14]. Murugan et al reported that the methanol extract of Achyranthes aspera (125 g/mL) produced biofilm inhibition of 94% [15].…”

“…One of these strategies can be using the various medicinal plants available [6]. Also, research on the anti-QS activity of medicinal plants has been rapidly increasing in recent years [14,15]. Evaluation of anti-QS and anti-biofilm activity of Murraya koenigii and Terminalia catappa against S. mutans can be done as both these plants have shown good antibacterial activity against S. mutans.…”