Anti-biofilm and wound-healing activity of chitosan-immobilized Ficin

Baidamshina, Diana R.; Koroleva, Victoria; Trizna, Elena Y.; Pankova, S. M.; Agafonova, Maria N.; Chirkova, Milana N.; Васильева, О. С.; Akhmetov, Nafis; Shubina, Valeriya V.; Porfiryev, Andrey G.; Семенова, Е. В.; Sachenkov, Oskar; Bogachev, Mikhail I.; Artyukhov, V. G.; Baltina, Tatyana; Holyavka, M. G.; Kayumov, Airat R.

doi:10.1016/j.ijbiomac.2020.09.030

Cited by 46 publications

(38 citation statements)

References 89 publications

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“…While embedded into the biofilm matrix, bacterial cells become largely inaccessible to both antibiotics and biocides. The above data suggest that biofilm matrix destruction with Longidaza ® could facilitate the penetration of antimicrobials into biofilm-embedded bacteria, as has been shown previously in other model investigations [33][34][35]66]. For this, 48-h old biofilms were incubated for either 4 or 24 h in the presence of Longidaza ® (750 IU) with antimicrobials (Ciprofloxacine and Cefuroxime) at their respective 1×, 4× and 16× MBCs (minimal bactericidal concentrations, see Table S1 for values), followed by the evaluation of the biofilm-embedded cells' viability with an MTT-assay (Figure 7).…”

Section: The Effect Of Longidaza ® On the Efficacy Of Antimicrobials Against Biofilm-embedded Bacteriasupporting

confidence: 82%

“…While various strategies have been offered to date for the targeting of topical biofilms for their destruction or the prevention of their formation [31][32][33][34], very few options are available for the treatment of urinary and urogenital biofilm-associated infections. One of the promising strategies for the destruction of already-formed biofilms seems to be their enzymatic treatment.…”

Section: The Effect Of Longidaza ® On the Bacterial Biofilms In Vitromentioning

confidence: 99%

“…To date, various strategies have been offered for targeting topical biofilms, focusing either on their destruction or on the prevention of their formation, or both [2,[31][32][33][34][35][36][37][38][39]. Nevertheless, very few options are available for the treatment of urinary biofilm-associated infections.…”

Section: Introductionmentioning

confidence: 99%

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Improving the Efficacy of Antimicrobials against Biofilm-Embedded Bacteria Using Bovine Hyaluronidase Azoximer (Longidaza®)

et al. 2021

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While in a biofilm, bacteria are extremely resistant to both antimicrobials and the immune system, leading to the development of chronic infection. Here, we show that bovine hyaluronidase fused with a copolymer of 1,4-ethylenepiperazine N-oxide and (N-carboxymethyl) -1,4-ethylenepiperazinium bromide (Longidaza®) destroys both mono- and dual-species biofilms formed by various bacteria. After 4 h of treatment with 750 units of the enzyme, the residual biofilms of Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae preserved about 50–70% of their initial mass. Biomasses of dual-species biofilms formed by S. aureus and the four latter species were reduced 1.5-fold after 24 h treatment, while the significant destruction of S. aureus–P. aeruginosa and S. aureus–K. pneumoniae was also observed after 4 h of treatment with Longidaza®. Furthermore, when applied in combination, Longidaza® increased the efficacy of various antimicrobials against biofilm-embedded bacteria, although with various increase-factor values depending on both the bacterial species and antimicrobials chosen. Taken together, our data indicate that Longidaza® destroys the biofilm structure, facilitating the penetration of antimicrobials through the biofilm, and in this way improving their efficacy, lowering the required dose and thus also potentially reducing the associated side effects.

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Section: The Effect Of Longidaza ® On the Efficacy Of Antimicrobials Against Biofilm-embedded Bacteriasupporting

confidence: 82%

Section: The Effect Of Longidaza ® On the Bacterial Biofilms In Vitromentioning

confidence: 99%

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Improving the Efficacy of Antimicrobials against Biofilm-Embedded Bacteria Using Bovine Hyaluronidase Azoximer (Longidaza®)

et al. 2021

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“…What is more, potential application of ficin in material modification or tissue engineering such as modify ficin on the surface of materials or incorporate ficin into scaffold could be further studied. For example, recent research showed both antibiofilm and wound-healing properties of chitosan-immobilized ficin [47].…”

Section: Biomed Research Internationalmentioning

confidence: 99%

The Inhibitory Effects of Ficin on Streptococcus mutans Biofilm Formation

Sun

Jiang

Zhang

et al. 2021

BioMed Research International

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To investigate the effects of ficin on biofilm formation of conditionally cariogenic Streptococcus mutans (S. mutans). Biomass and metabolic activity of biofilm were assessed using crystal violet assay, colony-forming unit (CFU) counting, and MTT assay. Extracellular polysaccharide (EPS) synthesis was displayed by SEM imaging, bacteria/EPS staining, and anthrone method while acid production was revealed by lactic acid assay. Growth curve and live/dead bacterial staining were conducted to monitor bacterial growth state in both planktonic and biofilm form. Total protein and extracellular proteins of S. mutans biofilm were analyzed by protein/bacterial staining and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), severally. qRT-PCR was conducted to detect acid production, acid tolerance, and biofilm formation associated genes. Crystal violet assay, CFU counting, and MTT assay showed that the suppression effect of ficin on S. mutans biofilm formation was concentration dependent. 4 mg/mL ficin had significant inhibitory effect on S. mutans biofilm formation including biomass, metabolic activity, EPS synthesis, and lactic acid production ( p < 0.05 ). The growth curves from 0 mg/mL to 4 mg/mL ficin were aligned with each other. There was no significant difference among different ficin groups in terms of live/dead bacterial staining result ( p > 0.05 ). Protein/bacterial staining outcome indicated that ficin inhibit both total protein and biofilm formation during the biofilm development. There were more relatively small molecular weight protein bands in extracellular proteins of 4 mg/mL ficin group when compared with the control. Generally, ficin could inhibit biofilm formation and reduce cariogenic virulence of S. mutans effectively in vitro; thus, it could be a potential anticaries agent.

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“…Thus, chitosan's high solubility in acidic solutions and its aggregation with polyanions give it excellent glue-forming properties [29]. Owing to its nontoxicity, biodegradability properties, and biocompatibility, it has been widely used as an excellent natural material in industrial and biomedical applications, such as for protein delivery [30], drug delivery [31], as a gene carrier [32], and for enzyme immobilization [33][34][35]. As in the above applications, the various forms that chitosan polymers take, such as beads/microspheres, microcapsules, fibers, membranes, coatings, sponges, and gels with different geometrical configurations, expand their use as carriers to support a large number of biomolecules or industrially relevant enzymes [36].…”

Section: Introductionmentioning

confidence: 99%

Chitosan Activated with Genipin: A Nontoxic Natural Carrier for Tannase Immobilization and Its Application in Enhancing Biological Activities of Tea Extract

et al. 2021

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In this work, a non-toxic chitosan-based carrier was constructed via genipin activation and applied for the immobilization of tannase. The immobilization carriers and immobilized tannase were characterized using Fourier transform infrared spectroscopy and thermogravimetric analysis. Activation conditions (genipin concentration, activation temperature, activation pH and activation time) and immobilizations conditions (enzyme amount, immobilization time, immobilization temperature, immobilization pH, and shaking speed) were optimized. The activity and activity recovery rate of the immobilized tannase prepared using optimal activation and immobilization conditions reached 29.2 U/g and 53.6%, respectively. The immobilized tannase exhibited better environmental adaptability and stability. The immobilized tannase retained 20.1% of the initial activity after 12 cycles and retained 81.12% of residual activity after 30 days storage. The catechins composition analysis of tea extract indicated that the concentration of non-ester-type catechins, EGC and EC, were increased by 1758% and 807% after enzymatic treatment. Biological activity studies of tea extract revealed that tea extract treated with the immobilized tannase possessed higher antioxidant activity, higher inhibitory effect on α-amylase, and lower inhibitory effect on α-glucosidase. Our results demonstrate that chitosan activated with genipin could be an effective non-toxic carrier for tannase immobilization and enhancing biological activities of tea extract.

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Anti-biofilm and wound-healing activity of chitosan-immobilized Ficin

Cited by 46 publications

References 89 publications

Improving the Efficacy of Antimicrobials against Biofilm-Embedded Bacteria Using Bovine Hyaluronidase Azoximer (Longidaza®)

Improving the Efficacy of Antimicrobials against Biofilm-Embedded Bacteria Using Bovine Hyaluronidase Azoximer (Longidaza®)

The Inhibitory Effects of Ficin on Streptococcus mutans Biofilm Formation

Chitosan Activated with Genipin: A Nontoxic Natural Carrier for Tannase Immobilization and Its Application in Enhancing Biological Activities of Tea Extract

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