Anti‐biofilm and cytoprotective activities of quercetin against<i>Pseudomonas aeruginosa</i>isolates

Vipin, C; Mujeeburahiman, M; Ashwini, P; Arun, A. B.; Rekha, P. D.

doi:10.1111/lam.13129

Cited by 40 publications

(22 citation statements)

References 33 publications

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“…Moreover, quercetin seems to restore corticosteroid sensitivity in cells from COPD patients by activation of the adenosine monophosphate-activated protein kinase (AMPK) [62], and a community clinical trial showed a reduction of upper respiratory tract infection total sick days and severity in middle age and older subjects ingesting 1000 mg quercetin/day for 12 weeks [63]. Quercetin antimicrobial effect has been previously reported [64][65][66] but, to our knowledge, this is the first study assessing its antimicrobial activity against H. influenzae.…”

Section: Discussionmentioning

confidence: 94%

Preclinical Evaluation of the Antimicrobial-Immunomodulatory Dual Action of Xenohormetic Molecules against Haemophilus influenzae Respiratory Infection

et al. 2019

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Chronic obstructive pulmonary disease (COPD) is characterized by abnormal inflammation and impaired airway immunity, providing an opportunistic platform for nontypeable Haemophilus influenzae (NTHi) infection. In this context, therapies targeting not only overactive inflammation without significant adverse effects, but also infection are of interest. Increasing evidence suggests that polyphenols, plant secondary metabolites with anti-inflammatory and antimicrobial properties, may be protective. Here, a Cistus salviifolius plant extract containing quercetin, myricetin, and punicalagin was shown to reduce NTHi viability. Analysis of these polyphenols revealed that quercetin has a bactericidal effect on NTHi, does not display synergies, and that bacteria do not seem to develop resistance. Moreover, quercetin lowered NTHi airway epithelial invasion through a mechanism likely involving inhibition of Akt phosphorylation, and reduced the expression of bacterially-induced proinflammatory markers il-8, cxcl-1, il-6, pde4b, and tnfα. We further tested quercetin’s effect on NTHi murine pulmonary infection, showing a moderate reduction in bacterial counts and significantly reduced expression of proinflammatory genes, compared to untreated mice. Quercetin administration during NTHi infection on a zebrafish septicemia infection model system showed a bacterial clearing effect without signs of host toxicity. In conclusion, this study highlights the therapeutic potential of the xenohormetic molecule quercetin against NTHi infection.

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Section: Discussionmentioning

confidence: 94%

Preclinical Evaluation of the Antimicrobial-Immunomodulatory Dual Action of Xenohormetic Molecules against Haemophilus influenzae Respiratory Infection

et al. 2019

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“…Previous studies demonstrated that quercetin can alter the cell surface properties to inhibit biofilm formation 41 – 43 . Thus, we assessed the cell surface properties by cell surface hydrophobicity and aggregation assays.…”

Section: Discussionmentioning

confidence: 99%

Quercetin inhibits virulence properties of Porphyromas gingivalis in periodontal disease

Zhang

et al. 2020

Sci Rep

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Porphyromonas gingivalis is a causative agent in the onset and progression of periodontal disease. This study aims to investigate the effects of quercetin, a natural plant product, on P. gingivalis virulence properties including gingipain, haemagglutinin and biofilm formation. Antimicrobial effects and morphological changes of quercetin on P. gingivalis were detected. The effects of quercetin on gingipains activities and hemolytic, hemagglutination activities were evaluated using chromogenic peptides and sheep erythrocytes. The biofilm biomass and metabolism with different concentrations of quercetin were assessed by the crystal violet and MTT assay. The structures and thickness of the biofilms were observed by confocal laser scanning microscopy. Bacterial cell surface properties including cell surface hydrophobicity and aggregation were also evaluated. The mRNA expression of virulence and iron/heme utilization was assessed using real time-PCR. Quercetin exhibited antimicrobial effects and damaged the cell structure. Quercetin can inhibit gingipains, hemolytic, hemagglutination activities and biofilm formation at sub-MIC concentrations. Molecular docking analysis further indicated that quercetin can interact with gingipains. The biofilm became sparser and thinner after quercetin treatment. Quercetin also modulate cell surface hydrophobicity and aggregation. Expression of the genes tested was down-regulated in the presence of quercetin. In conclusion, our study demonstrated that quercetin inhibited various virulence factors of P. gingivalis.

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“…For infection studies, HEK 293T cells were seeded onto 96-well plates (10,000 cells/well) in DMEM without the antibiotics and infected with the bacteria at a multiplicity of infection (MOI) of 10 for 3 h at 37 °C. Bacterial attachment to the HEK cells was estimated by the plate count method described earlier [ 50 ]. Briefly, the cells were washed with PBS, homogenized, serially diluted, and plated on NA.…”

Section: Methodsmentioning

confidence: 99%

First Report of Pathogenic Bacterium Kalamiella piersonii Isolated from Urine of a Kidney Stone Patient: Draft Genome and Evidence for Role in Struvite Crystallization

et al. 2020

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Uropathogenic bacteria are widely distributed in the environment and urinary tract infection is implicated in kidney stone disease. Here, we report on a urease negative bacterium Kalamiella piersonii (strain YU22) isolated from the urine of a struvite stone (MgNH4PO4·6H2O) patient. The closest species, K. piersonii IIIF1SW-P2T was reported from International Space Station samples. However, there are no earlier reports on its human association. Using whole genome and experimental analysis, its involvement in urinary tract colonization and struvite crystallization was explored. The strain YU22 showed many virulence factors that are needed for host cell invasion and colonization including cell adhesion factors, swimming and swarming motilities, biofilm and siderophore among others. In vitro infection studies in HEK-293T cells demonstrated the host cell attachment and killing. It was able to utilize amino acids as sole carbon source and showed growth in synthetic and healthy urine establishing metabolic adaptation to urinary tract. Increased pH and availability of ammonium ions from amino acid breakdown promoted struvite crystallization. The results from this study support the involvement of urease negative uropathogen in the struvite lithogenesis. Further studies on other isolates of K. peirsonii are warranted to assess its health risks.

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Anti‐biofilm and cytoprotective activities of quercetin againstPseudomonas aeruginosaisolates

Cited by 40 publications

References 33 publications

Preclinical Evaluation of the Antimicrobial-Immunomodulatory Dual Action of Xenohormetic Molecules against Haemophilus influenzae Respiratory Infection

Preclinical Evaluation of the Antimicrobial-Immunomodulatory Dual Action of Xenohormetic Molecules against Haemophilus influenzae Respiratory Infection

Quercetin inhibits virulence properties of Porphyromas gingivalis in periodontal disease

First Report of Pathogenic Bacterium Kalamiella piersonii Isolated from Urine of a Kidney Stone Patient: Draft Genome and Evidence for Role in Struvite Crystallization

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