Anti-biofilm and Antibacterial Activity of Allium sativum Against Drug Resistant Shiga-Toxin Producing Escherichia coli (STEC) Isolates from Patient Samples and Food Sources

Bhatwalkar, Sushma Bagde; Gound, Surendra Singh; Mondal, Rajesh; Srivastava, Rupesh K.; Anupam, Rajaneesh

doi:10.1007/s12088-019-00784-3

Cited by 20 publications

(16 citation statements)

References 52 publications

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“…Biofilm combating potential of AgNPs was evaluated as described by Bhatwalkar et al (2019) with some modifications. In brief, 200 µL of mid-log phase culture of B. cereus was grown in LB medium in 96 well plates and incubated at 37 °C for 24 h for biofilm formation.…”

Section: Anti-bacterial Propertiesmentioning

confidence: 99%

Characterization of biogenically synthesized silver nanoparticles for therapeutic applications and enzyme nanocomplex generation

2020

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The present study describes green synthesis of silver nanoparticles (AgNPs) and inulin hydrolyzing enzyme nanocomplexes (ENC) using Azadirachta indica (Ai) and Punica granatum (Pg) leaf extracts. Surface topology and physico-chemical characteristics of AgNPs were studied using surface plasmon resonance (SPR), FTIR, SEM, AFM and EDX analyses. Particle size analysis using dynamic light scattering and AFM studies revealed that Ai-AgNPs (76.4 nm) were spherical in shape having central bigger nano-regime with smaller surroundings while Pg-AgNPs (72.1 nm) and ENCs (Inulinase-Pg-AgNPs ~ 145 nm) were spherical particles having smooth surfaces. Pg-AgNPs exhibited significant photocatalysis of a thiazine dye, methylene blue. Both Ai-and Pg-AgNPs showed selective antibacterial action by inhibiting pathogenic Bacillus cereus, while the probiotic Lactobacillus strains remained unaffected. Ai-AgNPs showed potential anti-biofilm effect (30% viability) on B. cereus biofilms. Pg-AgNPs showed anti-cancer effect against human colon cancer cell lines (Caco-2) resulting in 40% cell death in 48 h. Enzymes (inulinase, L-asparaginase and glucose oxidase) were successfully immobilized onto nanoparticles together with the biogenic synthesis of AgNPs and recyclability of the Inulinase-Pg-AgNPs complex was demonstrated. The study elaborates characteristics of green synthesized nanoparticles and their potential applications as anti-cancer, antibacterial and antioxidant nano drugs that could be used in food and nutraceutical industries. Enzyme immobilization on AgNPs without any toxic cross-linker opens up newer possibilites in enzyme-nanocomplex research.

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Section: Anti-bacterial Propertiesmentioning

confidence: 99%

Characterization of biogenically synthesized silver nanoparticles for therapeutic applications and enzyme nanocomplex generation

2020

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“…Only samples without garlic and chili were collected, in order to avoid its documented potential antibacterial effect influencing the results [ 13 , 14 , 15 ].…”

Section: Methodsmentioning

confidence: 99%

A Pilot Survey on Hygienic–Sanitary Characteristics of Ready-To-Eat Sauces and Pesto

Caggiano

Diella

Trerotoli

et al. 2020

IJERPH

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In recent years, the chaotic habits of modern life have favored the consumption of quickly prepared meals, using ready-to-eat (RTE) foods and condiments. The aim of this study was to establish the microbiological safety of RTE sauces and pesto from markets analyzed at different stages of shelf life. In the bacterial investigation, all samples were shown to be acceptable, although differences were observed concerning shelf life times. On the other hand, the fungal investigation showed frequent positive results, with concentrations higher than threshold values. Detected microbial diffusion was the lowest when products were far from the expiry date and had just been opened, while high microbial proliferation was observed when analyzing the same package after 48 h, higher than for a product close to the end of its shelf life. This study highlights the discreet microbiological quality of processed and RTE foods, underlining the importance of hygienic–sanitary surveillance of these foods to their shelf life. Consequently, it is necessary to: (1) implement a food control plan for all food categories to carry out risk analysis associated with their consumption; and (2) better adapt the regulations relating to microbiological analysis, and understand the biological significance of each microbial parameter throughout the shelf life of foods.

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“…Garlic exhibits potent anti-microbial activity due to the production of allicin in damaged garlic tissues which inhibits bacterial infection in vapor form [109]. It also exhibits anti-bacterial and anti-biofilm potential against antibiotic resistant Shiga-Toxin producing Escherichia coli (STEC) isolates from food sources as well as from patient samples [110]. Since no volatile antibiotic is available till date that can treat pulmonary pathogenic infections, allicin could make a valuable addition to the presently available therapies.…”

Section: Allium Sativum (Garlic)mentioning

confidence: 99%

Phytotherapy for treatment of cytokine storm in COVID19

2021

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In 2020, a novel strain of coronavirus has led to a significant morbidity and mortality worldwide. As of the date of this writing, a total of 116 M cases has been diagnosed worldwide leading to 2.5 M deaths. The number of mortalities is directly correlated with the rise of innate immune cells (especially macrophages) in the lungs that secrete inflammatory cytokines (IL-1β and IL-6) leading to the development of "Cytokine Storm Syndrome" (CSS), multi-organ-failure and death. Given that currently the treatment of this condition is rare and release of effective vaccine might be months away, here, we review the plants and their pharmacologically active-compounds as potential phytopharmaceuticals for the virus induced inflammatory response. Experimental validation of the effectiveness of these natural compounds to prevent or reduce the cytokine storm might be beneficial as an adjunct treatment of SARS-CoV-2.

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Anti-biofilm and Antibacterial Activity of Allium sativum Against Drug Resistant Shiga-Toxin Producing Escherichia coli (STEC) Isolates from Patient Samples and Food Sources

Cited by 20 publications

References 52 publications

Characterization of biogenically synthesized silver nanoparticles for therapeutic applications and enzyme nanocomplex generation

Characterization of biogenically synthesized silver nanoparticles for therapeutic applications and enzyme nanocomplex generation

A Pilot Survey on Hygienic–Sanitary Characteristics of Ready-To-Eat Sauces and Pesto

Phytotherapy for treatment of cytokine storm in COVID19

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Anti-biofilm and Antibacterial Activity of Allium sativum Against Drug Resistant Shiga-Toxin Producing Escherichia coli (STEC) Isolates from Patient Samples and Food Sources

Cited by 20 publications

References 52 publications

Characterization of biogenically synthesized silver nanoparticles for therapeutic applications and enzyme nanocomplex generation

Characterization of biogenically synthesized silver nanoparticles for therapeutic applications and enzyme nanocomplex generation

A Pilot Survey on Hygienic–Sanitary Characteristics of Ready-To-Eat Sauces and Pesto

Phytotherapy for treatment of cytokine storm in COVID­19

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Product

Resources

About

Phytotherapy for treatment of cytokine storm in COVID19