Anti-biofilm activity of LC-MS based Solanum nigrum essential oils against multi drug resistant biofilm forming P. mirabilis

Khaled, Jamal M.; Alyahya, Sami A.; Kanisha, C. Chenthis; Alharbi, Naiyf S.; Kadaikunnan, Shine; Ramachandran, Govindan; Alanzi, Khalid F.; Rajivgandhi, Govindan; Vimala, R.T.V.; Manoharan, Natesan

doi:10.1016/j.sjbs.2020.10.003

Cited by 15 publications

(4 citation statements)

References 33 publications

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“…This first inquiry establishes the framework for a more extensive comprehension of the botanical specimen's chemical constitution, establishing the groundwork for later meticulous examinations, pharmacological investigations, and the study of prospective therapeutic utilities. 34…”

Section: Determination Of Extractive Valuesmentioning

confidence: 99%

Assessment of Cyperus articulatus for Antidiabetic Effects in a Streptozotocin-Induced Diabetic Rat Model

Dhar,

Datta,

Yadav

2024

IJPQA

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Hyperglycemia, polyuria, polydipsia, polyphagia, and renal glycosuria are all symptoms of diabetes mellitus (DM), also known as diabetes. Diabetic ketoacidosis, hyperosmolar hyperglycemia, there is a possibility that the illness could result in a number of different complications, some of which include cardiovascular disease, chronic renal disease, stroke, foot ulcers, visual damage, nerve damage, and cognitive impairment. The principal objective of this research was to regulate whether or not a methanolic extract of Cyperus articulates (MECA) rhizome possessed an antidiabetic effect. In order to evaluate the effectiveness of MECA as an antidiabetic agent, streptozotocin-induced diabetic rats were used in an experiment involving thiobarbituric acid reactive substances. In the aforementioned experiments, it was discovered that in streptozotocin (STZ) induced diabetic rats, MECA at doses of 200 and 400 mg/kg reduced blood glucose levels from ‘254.41 ± 11.7 to 98.50 ± 2.5 and 283.83 ± 27.8 to 96.35 ± 3 mg/dl respectively, while glibenclamide-treated rats had a decrease in blood glucose from 291.12 ± 17.1to 91.50 ± 1.7 mg. This decrease was statistically significant (p &LT; 0.05) when compared to the healthy control group. The effects of MECA on TBARS, GSH, and catalase in experimentally diabetic rats were found to be more pronounced than in the saline control group, and a greater rise in lipid peroxidation in the liver and kidney was seen in the diabetic group than in the saline group. Liver and kidney TBARS levels were observed to be considerably (p &LT; 0.05, p &LT; 0.001) reduced after MECA treatment, as were GSH levels in the STZ-control group and catalase activities in the experimental rats

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Section: Determination Of Extractive Valuesmentioning

confidence: 99%

Assessment of Cyperus articulatus for Antidiabetic Effects in a Streptozotocin-Induced Diabetic Rat Model

Dhar,

Datta,

Yadav

2024

IJPQA

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“…Khaled et al [47] used the agar well diffusion technique to validate the increase in antibacterial action of isolated solanum nigrum essential oils as opposed to biofilm-forming P. mirabilis. In vitro, studies strongly exhibited supported the use of solanum nigrum essential oils as a favourable antibiofilm agent as opposed to Gram-negative bacteria, especially P. mirabilis.…”

Section: Essential Oilsmentioning

confidence: 99%

“…Khaled et al [47] . used the agar well diffusion technique to validate the increase in antibacterial action of isolated solanum nigrum essential oils as opposed to biofilm‐forming P. mirabilis.…”

Section: Phytochemical Approaches For Controlling Antibiotics Resistancementioning

confidence: 99%

The Phytochemical Tactics for Battling Antibiotic Resistance in Microbes: Secondary Metabolites and Nano Antibiotics Methods

Adam

Tandon

et al. 2023

Chemistry & Biodiversity

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One of the most serious threats to human health is antibiotic resistance, which has left the world without effective antibiotics. While continuous research and inventions for new antibiotics are going on, especially those with new modes of action, it is unlikely that this alone would be sufficient to win the battle. Furthermore, it is also important to investigate additional approaches. One such strategy for improving the efficacy of existing antibiotics is the discovery of adjuvants. This review has collected data from various studies on the current crisis and approaches for combating multi‐drug resistance in microbial pathogens using phytochemicals. In addition, the nano antibiotic approaches, are discussed, highlighting the high potentials of essential oils, alkaloids, phenolic compounds, and nano antibiotics in combating antibiotic resistance.

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“…Development of host antibiotic resistance is caused mostly by their uncontrolled uses and this is mediated by increasing efflux pump activity, several modified enzymes which breaks the antibiotics and/or changing the target of antibiotics [ 2 , 3 ]. Due to the enhanced resistance, it becomes harder to treat common infectious diseases [ 2 , 4 ]. This situation is not only serious in medical indwelling-related hospital-acquired infections, but also been happening in serious magnitude in the communities [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Antibiofilm and anticancer activities of unripe and ripe Azadirachta indica (neem) seed extracts

Guchhait

Manna

Barai

et al. 2022

BMC Complement Med Ther

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Background Antibiotic resistances of pathogens and breast cancer warrant the search for new alternative strategies. Phytoextracts can eradicate microbe-borne diseases as well as cancer with lower side effects compared to conventional antibiotics. Aim Unripe and ripe Azadirachta indica (neem) seed extracts were explored as potential antibiofilm and anticancer agents in combating multidrug-resistant infectious bacteria as well as anticancer agents against the MDR breast cancer cell lines. Methods Shed-dried neem seeds (both unripe and ripe) were pulverized and extracted using methanol. The chemical components were identified with FTIR and gas chromatography - mass spectrometry. Antibiofilm activity of neem seed extracts were assessed in terms of minimum biofilm inhibitory concentration (MBIC), minimum biofilm eradication concentration (MBEC), and fluorescence microscopic studies on Staphylococcus aureus and Vibrio cholerae. Bacterial cells were studied by fluorescence microscopy using acridine orange/ethidium bromide as the staining agents. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were evaluated to observe the antibacterial activities. Cytotoxicity of the extracts against human blood lymphocytes and the anticancer activity against drug-resistant breast cancer cell lines were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and fluorescence-activated cell sorting (FACS) studies. Results 4-Ethyl-2-hydroxy-2-cyclopentene-1-one, phthalic acid, and 2-hexyl-tetrahydro thiophane were the major compounds in unripe neem seed, whereas 3,5-dihydroxy-6-methyl-2,3-dihydro-4-H-pyran-4-one and 4-ethylbenzamide were predominant in ripe neem seed. Triazine derivatives were also common for both the extracts. MBIC values of unripe and ripe neem seed extracts for S. aureus are 75 and 100 µg/mL, respectively, and for V. cholerae, they are 100 and 300 µg/mL, respectively. MBEC values of unripe and ripe seed extracts are 500 and 300 µg/mL, respectively for S. aureus and for V. cholerae the values are 700 and 500 µg/mL, respectively. Fluorescence microscopic studies at 16 and 24 h, after bacterial culture, demonstrate enhanced antibiofilm activity for the ripe seed extract than that of the unripe seeds for both the bacteria. MTT assay reveals lower cytotoxicity of both the extracts towards normal blood lymphocytes, and anticancer activity against breast cancer cell line (MDA-MB-231) with superior activity of ripe seed extract. FACS studies further supported higher anticancer activity for ripe seed extract. Conclusions Methanolic extract of neem seeds could substantially inhibit and eradicate biofilm along with their potent antibacterial and anticancer activities. Both the extracts showed higher antibiofilm and antibacterial activity against S. aureus (gram-positive) than V. cholerae (gram-negative). Moreover, ripe seed extract showed higher antibiofilm and anticancer activity than unripe extracts. Graphical Abstract

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Anti-biofilm activity of LC-MS based Solanum nigrum essential oils against multi drug resistant biofilm forming P. mirabilis

Cited by 15 publications

References 33 publications

Assessment of Cyperus articulatus for Antidiabetic Effects in a Streptozotocin-Induced Diabetic Rat Model

Assessment of Cyperus articulatus for Antidiabetic Effects in a Streptozotocin-Induced Diabetic Rat Model

The Phytochemical Tactics for Battling Antibiotic Resistance in Microbes: Secondary Metabolites and Nano Antibiotics Methods

Antibiofilm and anticancer activities of unripe and ripe Azadirachta indica (neem) seed extracts

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