Anti-biofilm activities of coumarin as quorum sensing inhibitor for <i>Porphyromonas gingivalis</i>

He, Zhaojian; Jiang, Wei; Jiang, Yijie; Dong, Jiachen; Zhang, Song; Xu, Jianrong; Wei, Zhou

doi:10.1080/20002297.2022.2055523

Cited by 17 publications

(16 citation statements)

References 61 publications

(69 reference statements)

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“…The Gram-negative anaerobic bacterium Porphyromonas gingivalis is considered the keystone pathogen in the development of periodontitis ( 6 ). P. gingivalis can colonize into the subgingival pocket to initiate the periodontitis ( 7 ), producing virulence factors, like gingipains and fimbrillin, to further damage the periodontal tissue ( 8 ), disrupt the balance of resident microbiota, and impair the host immune system ( 9 ).…”

Section: Introductionmentioning

confidence: 99%

Porphyromonas gingivalis Outer Membrane Vesicles Promote Apoptosis via msRNA-Regulated DNA Methylation in Periodontitis

Fan

Zhou

et al. 2023

Microbiol Spectr

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P. gingivalis is a bacterium often associated with periodontitis. This study demonstrates that (i) sRNA45033 in P. gingivalis OMVs targets CBX5, (ii) CBX5 regulates the methylation of p53 DNA and its expression, which is associated with apoptosis, and (iii) a novel mechanism of interaction between hosts and pathogens is mediated by OMVs in the occurrence of periodontitis.

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Section: Introductionmentioning

confidence: 99%

Porphyromonas gingivalis Outer Membrane Vesicles Promote Apoptosis via msRNA-Regulated DNA Methylation in Periodontitis

Fan

Zhou

et al. 2023

Microbiol Spectr

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“…The treatment of oral biofilm-related infections may also benefit from these QSI. For instance, a recent study showed that coumarin could inhibit P. gingivalis biofilm formation through a QS system [ 126 ]. In another study, curcumin decreased the expression of biofilm formation and virulence-related genes in both mono- and dual-species biofilms of S. mutans and C. albicans [ 127 ].…”

Section: Current Alternatives To Antibiotics To Prevent and Treat Ora...mentioning

confidence: 99%

Managing Oral Health in the Context of Antimicrobial Resistance

Bessa

Botelho

Machado

et al. 2022

IJERPH

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The oral microbiome plays a major role in shaping oral health/disease state; thus, a main challenge for dental practitioners is to preserve or restore a balanced oral microbiome. Nonetheless, when pathogenic microorganisms install in the oral cavity and are incorporated into the oral biofilm, oral infections, such as gingivitis, dental caries, periodontitis, and peri-implantitis, can arise. Several prophylactic and treatment approaches are available nowadays, but most of them have been antibiotic-based. Given the actual context of antimicrobial resistance (AMR), antibiotic stewardship in dentistry would be a beneficial approach to optimize and avoid inappropriate or even unnecessary antibiotic use, representing a step towards precision medicine. Furthermore, the development of new effective treatment options to replace the need for antibiotics is being pursued, including the application of photodynamic therapy and the use of probiotics. In this review, we highlight the advances undergoing towards a better understanding of the oral microbiome and oral resistome. We also provide an updated overview of how dentists are adapting to better manage the treatment of oral infections given the problem of AMR.

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“…The selection criteria of ligands were on the observation that essential oils demonstrated their ef icacy against QS of certain bacteria [15][16][17]. On the other hand, coumarin inhibited the QS of P.gingivalis in silico and in vitro [12].…”

Section: Ligands Preparationmentioning

confidence: 99%

“…This work suggests the application of the mentioned ligands as a means to control the bio ilm of P.gingivalis found within the oral cavity and its associated burden diseases such as dental plaques, Alzheimer's disease, and atherosclerosis. By approving their potency in silico, the best 2 ligands should ind their way in vitro setting to con irm the computational prediction by wet lab experiments as coupled by [12] .…”

Section: Ligand Binding Affi Nity Ligand Binding Affi Nitymentioning

confidence: 99%

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In silico disrupting quorum sensing of porphyromonas gingivalis via essential oils and coumarin derivatives

Al-Madhagi¹,

Samman²

2022

Ann Proteom Bioinform

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The emergence of porphyromonas gingivalis biofilm is a hallmark of risky burden diseases including Alzheimer's disease and atherosclerosis. The current study aims to screen some natural essential oil compounds and coumarin derivatives to interfere with quorum sensing of the bacterium and thus biofilm formation. A total of 20 ligands (10 essential oil molecules and 10 coumarin derivatives) were docked to P.gingivalis heme-binding protein HmuY using UCSF Chimera built-in AutoDock interface. Alongside, ADMET properties were also predicted via ADMETsar 2.0 and ProTox-II webservers. All of the selected ligands had higher free energy values than the reference inhibitor MES and native coumarin as well. Moreover, ADME parameters are in good agreement with Lipinski's rule of five. Nevertheless, the best molecules with top binding energy exhibited slight immunogenicity as well as carcinogenicity issues requiring in vitro confirmation. In conclusion, the tested ligands had better efficacy against P.gingivalis quorum sensing and biofilm.

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Anti-biofilm activities of coumarin as quorum sensing inhibitor for Porphyromonas gingivalis

Cited by 17 publications

References 61 publications

Porphyromonas gingivalis Outer Membrane Vesicles Promote Apoptosis via msRNA-Regulated DNA Methylation in Periodontitis

Porphyromonas gingivalis Outer Membrane Vesicles Promote Apoptosis via msRNA-Regulated DNA Methylation in Periodontitis

Managing Oral Health in the Context of Antimicrobial Resistance

In silico disrupting quorum sensing of porphyromonas gingivalis via essential oils and coumarin derivatives

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