Anti-bilirubin monoclonal antibody I. Preparation and properties of monoclonal antibodies to covalently coupled bilirubin-albumin

Shimizu, Shuji; Izumi, Yasuharu; Yamazaki, Masahiko; Shimizu, Kuniko; Yamaguchi, Tokio; Nakajima, Hiroshi

doi:10.1016/0304-4165(88)90017-7

Cited by 26 publications

(12 citation statements)

References 16 publications

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“…No. B433, Shino-test Corp., Kanagawa, Japan) (26,27). The results were expressed relative to urinary creatinine concentrations, which were measured enzymatically (Mitsubishi Chemical Medience, Tokyo, Japan).…”

Section: Measurement Of Urinary Biopyrrinmentioning

confidence: 99%

“…The results were expressed relative to urinary creatinine concentrations, which were measured enzymatically (Mitsubishi Chemical Medience, Tokyo, Japan). The biopyrrin immunoassay using 24G7 monoclonal antibody is influenced by hyperbilirubinemia (26,27); therefore, the measured urinary biopyrrin level may be influenced by the serum direct bilirubin level. Therefore, the latter was measured by a chemical oxidation-based method (Mitsubishi Chemical Medience, Tokyo, Japan).…”

Section: Measurement Of Urinary Biopyrrinmentioning

confidence: 99%

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Effects of lifestyle factors on urinary oxidative stress and serum antioxidant markers in pregnant Japanese women: A cohort study

Matsuzaki

Haruna

Ota

et al. 2014

BST

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Section: Measurement Of Urinary Biopyrrinmentioning

confidence: 99%

Section: Measurement Of Urinary Biopyrrinmentioning

confidence: 99%

Effects of lifestyle factors on urinary oxidative stress and serum antioxidant markers in pregnant Japanese women: A cohort study

Matsuzaki

Haruna

Ota

et al. 2014

BST

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“…The antibilirubin mAb 24 (kindly provided by M. Suematsu, Keio University, Tokyo, Japan) was used to analyze HO-1 enzymatic activity in 0.1% Triton-permeabilized DCs. Staining was performed as described.…”

Section: Immunohistology and Confocal Image Analysismentioning

confidence: 99%

Heme oxygenase-1 expression inhibits dendritic cell maturation and proinflammatory function but conserves IL-10 expression

Chauveau

Remy

Royer

et al. 2005

Blood

313

299

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Heme oxygenase-1 (HO-1) is an intracellular enzyme that degrades heme and inhibits immune responses and inflammation in vivo. In most cell types, HO-1 is inducible by inflammatory stimuli and oxidative stress. Here we demonstrate that human monocyte-derived immature dendritic cells (iDCs) and several but not all freshly isolated rat splenic DC subsets and rat bone marrow-derived iDCs, spontaneously express HO-1. HO-1 expression drastically decreases during human and rat DC maturation induced in vitro. In IntroductionHeme oxygenases (HOs) are the rate-limiting intracellular enzymes that degrade heme to biliverdin, free divalent iron, and CO (for a review, see Otterbein and Choi 1 ). Three distinct HO enzymes have been identified: HO-1, HO-2, and HO-3. 1 HO-1 is a stress responsive gene whose expression is induced by a variety of stimuli including heme, heavy metals, inflammatory cytokines, and nitric oxide. 1 HO-1 is known for its cytoprotective effect against oxidative injuries and inflammation. 1 Induction of HO-1 expression by pharmacologic activators or gene transfer has had therapeutic effects in a variety of conditions or disorders involving the immune system, including transplantation and inflammatory disorders. [2][3][4][5][6][7][8] Biliverdin and its metabolite, bilirubin, are known for their antioxidant 9 and immunosuppressive effects. 10 HO-1 and CO have been shown to inhibit lipopolysaccharide (LPS)-induced expression of proinflammatory cytokines and to increase LPS-induced expression of interleukin 10 (IL-10) in macrophages. 11,12 Moreover, IL-10 induces HO-1 expression in macrophages. [13][14][15] We previously reported that overexpression of HO-1, obtained with an HO-1-encoding adenovirus in rats having heart transplants, results in long-term allograft survival associated with an inhibition of cellular allogeneic immune responses, which could be mediated by adenoviral transduction of dendritic cells (DCs). 6 DCs play a central role in the induction of immunity and tolerance (for a review, see Steinman et al 16 ). In the absence of inflammation, immature DCs (iDCs) located in peripheral tissues specialize in taking up innocuous and cell-associated self antigens.They continuously capture antigens and migrate to draining lymph nodes where they can induce tolerance. 16 In the presence of danger signals, DCs undergo maturation, a process involving upregulation of surface major histocompatibility complex (MHC) class II and costimulatory molecules, secretion of proinflammatory and anti-inflammatory cytokines, and the acquired ability to stimulate differentiation of naive T cells into effector cells.Our working hypothesis was that DCs can express HO-1, which can regulate DC functions. In this study, we demonstrate that human and rat iDCs express HO-1 and that HO-1 expression is down-regulated by maturation stimuli. Our results also demonstrate that induction of HO-1 expression renders DCs refractory to LPS-induced maturation, but preserves IL-10 secretion, suggesting that HO-1 may be used to regulate DC f...

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“…However, many reports have shown the preparation of antibodies against lowmolecular-weight haptens such as drugs, 33,34) nutrients, 35) and endogenous metabolites. [36][37][38][39] In most cases, to prepare immunogens, haptens or analogous derivatives were coupled with carrier proteins such as keyhole limpet hemocyanin (KLH), bovine serum albumin (BSA), or ovalbumin through amide-bond formation using carbodiimide reagents. To prepare anti-polyphenol antibodies, we utilized chemically synthesized Q3GA 24) as the immunogen, because (i) glucuronide metabolites are the most abundant forms of quercetin 23,24) and other polyphenols in vivo, and (ii) glucuronide metabolites have one carboxyl group in their intact structures available for coupling with carrier proteins.…”

Section: Anti-polyphenol Monoclonal Antibodiesmentioning

confidence: 99%

Immunochemical Detection of Food-Derived Polyphenols in the Aorta: Macrophages as a Major Target Underlying the Anti-Atherosclerotic Activity of Polyphenols

Kawai

2011

Bioscience, Biotechnology, and Biochemistry

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Anti-bilirubin monoclonal antibody I. Preparation and properties of monoclonal antibodies to covalently coupled bilirubin-albumin

Cited by 26 publications

References 16 publications

Effects of lifestyle factors on urinary oxidative stress and serum antioxidant markers in pregnant Japanese women: A cohort study

Effects of lifestyle factors on urinary oxidative stress and serum antioxidant markers in pregnant Japanese women: A cohort study

Heme oxygenase-1 expression inhibits dendritic cell maturation and proinflammatory function but conserves IL-10 expression

Immunochemical Detection of Food-Derived Polyphenols in the Aorta: Macrophages as a Major Target Underlying the Anti-Atherosclerotic Activity of Polyphenols

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