Anti-beta 2 glycoprotein I antibodies in centenarians

Abstract

Background: Non-organ-specific autoantibodies are present in centenarians without evidence of autoimmune diseases but conflicting or no data on anti-phospholipid and anti-phospholipid binding proteins were reported.Objective: To investigate the presence and antigen specificity of anti-phospholipid and anti-phospholipid binding proteins in centenarians. Methods: Seventy-seven centenarians, 70 adult controls, 65 unselected elderly subjects, and 38 old SENIEUR volunteers were investigated. Anti-cardiolipin, anti-human b2glycoprotein I, and lupus anticoagulant were detected. Antigen specificity was assayed against plates coated with anionic, neutral and cationic phospholipids and b2glycoprotein I-dependence was also evaluated.Results: 54.3% of the centenarians were positive for IgG and 8.6% for IgM anti-b2glycoprotein I antibodies, while only 20.7% centenarians were positive for anti-cardiolipin IgG and 2.59% for IgM; none resulted positive for lupus anticoagulant. Anti-cardiolipin positive sera cross-reacted with negatively charged phospholipids and displayed decreased binding to serum-free cardiolipin-coated plates that was restored by human b2glycoprotein I or fetal calf serum.Conclusions: Centenarians display high reactivity against human b2glycoprotein I but low binding to the bovine molecule in the anticardiolipin assay. In spite of the presence of antibodies comparable to those found in patients with the anti-phospholipid syndrome, no vascular events were reported suggesting the presence of unknown protective factors and/or the lack of triggering factors. q

Thrombus formation induced by antibodies to β2-glycoprotein I is complement dependent and requires a priming factor

“…In addition, they displayed medium-high titer of anti-␤2GPI antibodies detected as described in a previous publication. 12 The clinical features and the level of aPL, anti-␤2GPI, and C-fixing antibodies are reported in Table 1. Anticardiolipin and anti-␤2GPI antibodies were detected by enzymelinked immunosorbent assay (ELISA), and values were expressed as IgG antiphospholipid units (GPL) or OD, respectively, as described.…”

Serum Concentration of Immunoglobulin Classes and Subclasses in Macedonian Elderly People

“…Centenarians display high reactivity against human beta 2 glycoprotein I but low binding to the bovine molecule in the anti-cardiolipin assay. In spite of the presence of antibodies comparable to those found in patients with the anti-phospholipid syndrome, no vascular events were reported suggesting the presence of unknown protective factors and/or the lack of triggering factors [11].…”

“…The antiinfluenza vaccine induced increase in TNFalpha and IL10 in the all non-responders, while cortisol increased only in the young. They concluded that CMV carrier status eliciting elevated proinflammatory potential could contribute to unresponsiveness to the anti-influenza vaccine [11].…”

La malattia reumatica nel paziente anziano

“…In un altro studio che includeva 77 centenari, 65 soggetti anziani sani (età 65-75) e 70 soggetti sani di età compresa tra 25 e 45 anni, Meroni et al [33] hanno evidenziato che il 54% dei centenari erano positivi per anticorpi anti-β 2 GPI di classe IgG e 8,6% per anti-β 2 GPI IgM, mentre solamente il 21% dei soggetti era positivo per aCL IgG e solo il 3% per aCL IgM (Tab. 7).…”