Anti-bacterial mechanism of the P-3 against Aeromonas hydrophila NJ-35 extracted from the Potentilla chinensis Ser. (Wei Ling Cai)

Li, Zhenzhen; He, Lili; He, Liren; Chen, Fuxing; Song, Suquan; Zhang, Hang; Sun, Wuping; Bao, Xiaowei; Zhang, Haibin; Li, Tuo; He, Chenghua

doi:10.1016/j.aquaculture.2021.736746

Cited by 3 publications

(1 citation statement)

References 49 publications

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“…Hemolysins and proteases are the crucial extracellular virulence factors produced by A. hydrophila [48]. By damaging host tissues, these enzymes allow pathogens to gain nutrients and spread [49].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial and Antivirulence Activities of Carvacrol against Pathogenic Aeromonas hydrophila

et al. 2022

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Aeromonas hydrophila is a ubiquitous Gram-negative opportunistic pathogen in the freshwater environment and the most common cause of bacterial septicemia in aquaculture. In this study, we investigated the impact of carvacrol, a natural monoterpenoid found in herbs, on the virulence of A. hydrophila in vitro and the antibacterial effect in combination with antibiotics. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of carvacrol against A. hydrophila NJ-35 were 125 µg/mL and 250 µg/mL, respectively. Carvacrol could inhibit the virulence factors (biofilm, protease, exopolysaccharide, and hemolysin) of A. hydrophila, and the antibiofilm potential of carvacrol was further verified by microscopic detection. Transcriptional analyses showed that the gene expression of flaB, ompA, aha, ahp, ela, act, aerA, AhyR, and hly were marked as downregulated. The checkerboard assay results showed that carvacrol did not have an antagonistic effect in combination with antibiotics (florfenicol, enrofloxacin, thiamphenicol, or doxycycline hydrochloride) commonly used in aquaculture but possessed an additive-synergistic effect with neomycin sulfate. In vivo studies demonstrated that carvacrol protected grass carp (Ctenopharyngodon idella) from A. hydrophila infection. Our results indicated that carvacrol possessed significant anti-bacterial and anti-virulence effects on A. hydrophila.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial and Antivirulence Activities of Carvacrol against Pathogenic Aeromonas hydrophila

et al. 2022

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Antibacterial activities of Adina rubella extract enhanced by fermentation and its application in packaging films

Lei,

Jiang,

Yao

2024

Food Chemistry

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The combined effect of a novel formula of herbal extracts on bacterial infection and immune response in Micropterus salmoides

et al. 2023

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Herbal extracts have been considered as ideal alternative to antibiotics in aquaculture and application of combinatory effective extracts always can exhibit the enhanced bioactivity with high efficiency. In our study, a novel herbal extract combination GF-7, which is composed of Galla Chinensis, Mangosteen Shell extracts as well as the effective parts of Pomegranate peel and Scutellaria baicalensis Georgi extracts, was prepared and applied for the therapy of bacterial infection in aquaculture. The HPLC analysis of GF-7 was also investigated for quality control and chemical identification. In the bioassay, GF-7 had excellent antibacterial activity against various aquatic pathogenic bacteria in vitro, and the related MIC values were between 0.045 and 0.36 mg/mL. After feeding Micropterus salmoide with GF-7 (0.1, 0.3, and 0.6%, respectively) for 28 days, the activities of ACP, AKP, LZM, SOD, and CAT of the liver in each treatment group were significantly increased and the content of MDA was significantly decreased. Meanwhile, the hepatic expression of the immune regulators including IL-1β, TNF-α, and Myd88 at different times was up-regulated in varying degrees. The challenge results exhibited a good dose-dependent protective effect on M. salmoides infected with A. hydrophila, which was further confirmed by liver histopathology. Our results imply that the novel combination GF-7 is a potential natural medicine for the prevention and treatment of numerous aquatic pathogenic infectious diseases in aquaculture.

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Anti-bacterial mechanism of the P-3 against Aeromonas hydrophila NJ-35 extracted from the Potentilla chinensis Ser. (Wei Ling Cai)

Cited by 3 publications

References 49 publications

Antimicrobial and Antivirulence Activities of Carvacrol against Pathogenic Aeromonas hydrophila

Antimicrobial and Antivirulence Activities of Carvacrol against Pathogenic Aeromonas hydrophila

Antibacterial activities of Adina rubella extract enhanced by fermentation and its application in packaging films

The combined effect of a novel formula of herbal extracts on bacterial infection and immune response in Micropterus salmoides

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