Anti-bacterial activity of baicalin against APEC through inhibition of quorum sensing and inflammatory responses

Peng, Lu-Yuan; Yuan, Meng; Wu, Zong-Mei; Song, Ke; Zhang, Chunlei; An, Qiang; Xia, Fang; Yu, Jialin; Yi, Pengfei; Fu, Binying; Shen, Haiqing

doi:10.1038/s41598-019-40684-6

Cited by 44 publications

(37 citation statements)

References 41 publications

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“…The expression of phosphorylated NF-κB p65 and phosphorylated IκB was significantly upregulated in the APEC-infected chicken type II pneumocytes compared with the control group. However, baicalin, a medicinal ingredient isolated from dry roots of Scutellaria baicalensis Georgi, was shown to significantly inhibit the expression of phosphorylated NF-κB p65 and phosphorylated IκB induced by APEC-O78 [ 267 ].…”

Section: Effect Of Various Stress Factors On Nf-κb Expression and mentioning

confidence: 99%

Redox Homeostasis in Poultry: Regulatory Roles of NF-κB

2021

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Redox biology is a very quickly developing area of modern biological sciences, and roles of redox homeostasis in health and disease have recently received tremendous attention. There are a range of redox pairs in the cells/tissues responsible for redox homeostasis maintenance/regulation. In general, all redox elements are interconnected and regulated by various means, including antioxidant and vitagene networks. The redox status is responsible for maintenance of cell signaling and cell stress adaptation. Physiological roles of redox homeostasis maintenance in avian species, including poultry, have received limited attention and are poorly characterized. However, for the last 5 years, this topic attracted much attention, and a range of publications covered some related aspects. In fact, transcription factor Nrf2 was shown to be a master regulator of antioxidant defenses via activation of various vitagenes and other protective molecules to maintain redox homeostasis in cells/tissues. It was shown that Nrf2 is closely related to another transcription factor, namely, NF-κB, responsible for control of inflammation; however, its roles in poultry have not yet been characterized. Therefore, the aim of this review is to describe a current view on NF-κB functioning in poultry with a specific emphasis to its nutritional modulation under various stress conditions. In particular, on the one hand, it has been shown that, in many stress conditions in poultry, NF-κB activation can lead to increased synthesis of proinflammatory cytokines leading to systemic inflammation. On the other hand, there are a range of nutrients/supplements that can downregulate NF-κB and decrease the negative consequences of stress-related disturbances in redox homeostasis. In general, vitagene–NF-κB interactions in relation to redox balance homeostasis, immunity, and gut health in poultry production await further research.

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Section: Effect Of Various Stress Factors On Nf-κb Expression and mentioning

confidence: 99%

Redox Homeostasis in Poultry: Regulatory Roles of NF-κB

2021

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“…The inflammatory response is normally identified as the main line of defense against pathogenic invasion, but the inflammatory response also serves as the major pathological feature of APEC invasion ( Peng et al., 2019 ). Thus, inhibition of inflammation plays an active role in APEC prevention.…”

Section: Introductionmentioning

confidence: 99%

Baicalin ameliorates APEC-induced intestinal injury in chicks by inhibiting the PI3K/AKT-mediated NF-κB signaling pathway

Cheng¹,

Cao²,

Luo³

et al. 2022

Poultry Science

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Avian pathogenic Escherichia coli ( APEC ) is the causative agent of avian colibacillosis. Baicalin ( BA ) possesses multiple pharmacological effects, but the mechanism underlying its activity in APEC-induced intestinal injury remains unknown. This study aims to investigate the protective effects and possible mechanism of BA against APEC-induced intestinal injury. Sixty 1-day-old chicks were randomly divided into 4 groups: the control group (basal diet), E. coli group (basal diet), BAI10 group (10 mg/kg BA), and BAI20 group (20 mg/kg BA). After pretreatment with BA for 15 d and subsequent induction of APEC infection by pectoralis injection, the ileum was collected and analyzed. The results showed that BA-pretreatment demonstrated an alleviation of chicks in diarrhea rate, mortality, and histopathological changes in intestinal tissues after APEC infection. Additionally, following APEC infection, BA improved the intestinal barrier by elevating zona occludens ( ZO )s (ZO-1, 2, 3), Claudins (Claudin1, 2, 3), Occludin, avian β-defensin ( AvBD )s (AvBD1, 2, 4), lysozyme ( Lyz ) mRNA levels and ZO-1, Claudin1, and Occludin protein levels. Besides, the activities of total superoxide dismutase ( T-SOD ), catalase ( CAT ), and glutathione peroxidase ( GSH-Px ) and the SOD-1 and CAT mRNA levels and SOD-1 protein level were elevated by BA pretreatment. BA pretreatment also decreased the malondialdehyde ( MDA ) content, heme oxygenase-1 ( HO-1 ) and NADH quinone oxidoreductase 1 ( NQO1 ) mRNA levels, and HO-1 protein level after APEC infection. BA alleviated the APEC-induced inflammatory response, including downregulating the mRNA levels of proinflammatory cytokines (tumor necrosis factor-α ( TNF-α ), interleukin [ IL ]-1β, IL-6, IL-8) and upregulating the mRNA levels of anti-inflammatory cytokines (IL-4, IL-10, IL-13, transforming growth factor-β [ TGF-β ]). Furthermore, BA decreased the mRNA and protein levels of phosphatidylinositol 3 kinase ( PI3K ), protein kinase B ( AKT ), and nuclear factor kappa-B ( NF-κB ) as well as the expression of the phosphorylated forms of these proteins after APEC infection. Collectively, our findings indicate that BA exerts a protective effect against APEC-induced intestinal injury in chicks by inhibiting the PI3K/AKT-mediated NF-κB pathway, suggesting that BA may be a potential therapeutic approach for avian colibacillosis.

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“…Furthermore, from the systemic context, baicalein has demonstrated hepatoprotective properties [ 20 ]. There is evidence to show the anti-quorum sensing activity and biofilm inhibitory potential of Baicalein against Staphylococcus aureus [ 21 ], Avian Pathogenic E. coli (APEC) [ 22 ] and Candida albicans [ 23 ]. Based on this premise, we hypothesised that baicalein inhibits S. mutans biofilms and its virulence.…”

Section: Introductionmentioning

confidence: 99%

Baicalein Inhibits Streptococcus mutans Biofilms and Dental Caries-Related Virulence Phenotypes

et al. 2021

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Dental caries, the most common oral disease, is a major public healthcare burden and affects more than three billion people worldwide. The contemporary understanding of the need for a healthy microbiome and the emergence of antimicrobial resistance has resulted in an urgent need to identify compounds that curb the virulence of pathobionts without microbial killing. Through this study, we have demonstrated for the first time that 5,6,7-trihydroxyflavone (Baicalein) significantly downregulates crucial caries-related virulence phenotypes in Streptococcus mutans. Baicalein significantly inhibited biofilm formation by Streptococcus mutans UA159 (MBIC50 = 200 μM), without significant growth inhibition. Notably, these concentrations of baicalein did not affect the commensal S. gordonii. Strikingly, baicalein significantly reduced cell surface hydrophobicity, autoaggregation and acid production by S. mutans. Mechanistic studies (qRT-PCR) showed downregulation of various genes regulating biofilm formation, surface attachment, quorum sensing, acid production and competence. Finally, we demonstrate the potential translational value of baicalein by reporting synergistic interaction with fluoride against S. mutans biofilms.

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Anti-bacterial activity of baicalin against APEC through inhibition of quorum sensing and inflammatory responses

Cited by 44 publications

References 41 publications

Redox Homeostasis in Poultry: Regulatory Roles of NF-κB

Redox Homeostasis in Poultry: Regulatory Roles of NF-κB

Baicalin ameliorates APEC-induced intestinal injury in chicks by inhibiting the PI3K/AKT-mediated NF-κB signaling pathway

Baicalein Inhibits Streptococcus mutans Biofilms and Dental Caries-Related Virulence Phenotypes

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