Anti‐asthmatic Effects of Baicalin in a Mouse Model of Allergic Asthma

Abstract

The aim of the study was to investigate the anti-asthmatic effects of baicalin (BA) and the possible mechanisms. Asthma model was established by ovalbumin (OVA) intraperitoneal injection. A total of 60 mice were randomly assigned to six experimental groups: control, model, dexamethasone (2 mg/kg), and BA (10 mg/kg, 20 mg/kg, 40 mg/kg). Airway resistance (RI) and lung compliance (Cdyn) were measured, histological studies were evaluated by the hematoxylin and eosin staining, Th1/Th2, OVA-specific serum, and BALF IgE levels and Th17 cytokines were evaluated by enzyme-linked immunosorbent assay, and Th17 cells was evaluated by flow cytometry (FCM). Our study demonstrated that BA inhibited OVA-induced increases in RI and eosinophil count; interleukin (IL)-4, IL-17A levels, and Cdyn were recovered and increased IFN-γ level in bronchoalveolar lavage fluid. Histological studies demonstrated that BA substantially inhibited OVA-induced eosinophilia in lung tissue and airway tissue. FCM studies demonstrated that BA substantially inhibited Th17 cells. These findings suggest that BA may effectively ameliorate the progression of asthma and could be used as a therapy for patients with allergic asthma.

Baicalin attenuates inflammation in mice with OVA-induced asthma by inhibiting NF-κB and suppressing CCR7/CCL19/CCL21

“…In a previous study of ours, we demonstrated the anti-airway remodeling effect of baicalin (16). Moreover, baicalin has been shown to rectify the Th1/Th2 and Treg/Th17 imbalance in asthmatic mice (17). However, the effects and related molecular mechanisms of action of baicalin on CCR7 and its ligands associated with airway inflammation have not been fully elucidated.…”

Baicalin inhibits biofilm formation, attenuates the quorum sensing-controlled virulence and enhances Pseudomonas aeruginosa clearance in a mouse peritoneal implant infection model

“…However, a shift from the Th2 to the Th1 immune response may promote bacterial clearance and improve the prognosis of infected individuals [69,70]. According to Ma et al, baicalin treatment effectively reduced IL-4 levels and increased IFN-γ levels in the bronchoalveolar lavage fluid in a mouse model of allergic asthma [71]. Likewise, based on the results of the present study, baicalin significantly reduced the production of IL-4 and enhanced the generation of IFN-γ in the lavage fluid collected from the peritoneal cavity, reversing the Th1/Th2 balance to a Th1-dominated state.…”

Spectroscopic investigation on the food components–drug interaction: The influence of flavonoids on the affinity of nifedipine to human serum albumin

“…Rutin is known to exhibit multiple pharmacological activities including antioxidant, anti-inflammation, anti-microbial, anti-tumor and anti-asthma (Chua, 2013;Koval'skii et al, 2014). Similarly, baicalin has been reported to show widely pharmacological and therapeutic properties including antiinflammatory, anti-adipogenesis, anti-asthma, anti-tumor, antibacterial and anti-diarrhea Cui et al, 2014;Ma et al, 2014). Because of the potential beneficial effects of flavonoids on human health, foods rich in flavonoids have attracted great interest recently (Kozłowska and Szostak-Wegierek, 2014;Xiao et al, 2014).…”