Anti-asthmatic activity of standardized hydro-ethanolic and aqueous extracts of Stachytarpheta cayennensis (Rich.) Vahl in a murine model

Guimarães, Camila Carla; Sandy, Camila Mira; Figueira, Rebeca Lopes; Gonçalves, Mirani da Rocha; Crevelin, Eduardo José; Cardoso, Pedro Henrique; Fabro, Alexandre Todorovic; Carmona, Fábio; Borges, Marcos C.; Pereira, Ana Maria Soares

doi:10.1016/j.jep.2022.115877

Cited by 5 publications

(2 citation statements)

References 49 publications

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“…[7][8][9][10] The previous study has demonstrated that intraperitoneal injection of OVA and alum once a week for 2 weeks is the most straightforward and effective approach to induce an asthmatic reaction in mice. 11 However, the inconsistent use of sensitizing OVA doses in current studies [12][13][14] and the lack of research on their effect on AHR necessitate further investigation. Therefore, we conducted a study using increasing doses (0, 10, 20, 50, and 100 μg) of sensitizing OVA in a BALB/c murine model of asthma to evaluate their impact on AHR to methacholine (MCh), as well as histological changes, eosinophilic infiltration, and epithelial damage.…”

Section: Introductionmentioning

confidence: 99%

Effects of increasing sensitizing doses of ovalbumin on airway hyperresponsiveness in asthmatic mice

Chen,

Yuan,

Peng

et al. 2024

Immunity Inflam & Disease

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BackgroundThe dosage of ovalbumin (OVA) during the sensitization stage is considered a crucial factor in the development of airway hyperresponsiveness (AHR). However, the inconsistent dosages of sensitizing OVA used in current studies and the lack of research on their impact on AHR are notable limitations.MethodsWe examined the impact of increasing sensitizing doses of OVA in a murine asthma model, which entailed initial sensitization with OVA followed by repeated exposure to OVA aerosols. BALB/c mice were primed with doses of OVA (0, 10, 20, 50, and 100 μg) plus 1 mg Alum on Days 0 and 7, and were challenged with OVA aerosols (10 mg/mL for 30 min) between Days 14 and 17. Antigen‐induced AHR to methacholine (MCh), as well as histological changes, eosinophilic infiltration, and epithelial injury were assessed.ResultsThe result indicated that there are striking OVA dose‐related differences in antigen‐induced AHR to MCh. The most intense antigen‐induced AHR to MCh was observed with sensitization at 50 μg, while weaker responses were seen at 10, 20, and 100 μg. Meanwhile, there was a significant increase in eosinophil count with sensitization at 50 μg. The changes of AHR were correlated with total cells count, lymphocytes count, eosinophils count, and basophils count in bronchoalveolar lavage fluid; however, it did not correlate with histological changes such as cellular infiltration into bronchovascular bundles and goblet cell hyperplasia of the bronchial epithelium.ConclusionOverall, this study demonstrated that sensitization with 50 μg of OVA resulted in the most significant AHR compared to other dosages. These findings may offer valuable insights for future research on mouse asthma modeling protocols.

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Section: Introductionmentioning

confidence: 99%

Effects of increasing sensitizing doses of ovalbumin on airway hyperresponsiveness in asthmatic mice

Chen,

Yuan,

Peng

et al. 2024

Immunity Inflam & Disease

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“…It is also helpful for the treatment of pain, dysentery, and liver diseases. Tea made from the leaves is popularly used to control blood sugar levels by people in Peru and other Latin countries (Guimarães et al, 2023;Okokon et al, 2008;Olayode et al, 2020). Alkaloids, terpenoids, tannins, saponins, glycosides, gluconic acid, steroids, and phenolic compounds were the reported phytochemicals isolated from S. cayennensis (Adebajo et al, 2007;Ho and BO, 2005;Onofre et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Analgesic, Antioxidant, Cytotoxic and Thrombolytic Potential of Stachytarpheta cayennensis (Rich.) Vahl Leaves

Jaman¹,

Akhter²,

Shahriar³

et al. 2023

Bangla Pharma J

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The current study was undertaken for the preliminary investigation of the analgesic, cytotoxic, antioxidant, and thrombolytic properties of the methanolic extract of Stachytarpheta cayennensis (Rich.) Vahl leaves. Both the central and peripheral analgesic action of the crude extract of methanol were significant and the analgesic effect increases with the increasing of dose and time with the pronounced effect at 600 mg/kg body weight. According to the brine shrimp lethality bioassay, the pet-ether soluble fraction (PESF) displayed notable cytotoxic action showing LC50 value (5.33 μg/ml) among the fractions as compared to the standard vincristine sulfate (0.452 μg/ml) and crude extract of the leaves contained a total phenolic content ranging from 18.68-145.98 mg of GAE/gm while revealing moderate thrombolytic activity. Bangladesh Pharmaceutical Journal 26(1): 45-50, 2023 (January)

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In silico analysis of plants biomass phytochemicals against β2 adrenergic receptor

Lavanya

Namasivayam

Praveena³

et al. 2023

Biomass Conv. Bioref.

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Anti-asthmatic activity of standardized hydro-ethanolic and aqueous extracts of Stachytarpheta cayennensis (Rich.) Vahl in a murine model

Cited by 5 publications

References 49 publications

Effects of increasing sensitizing doses of ovalbumin on airway hyperresponsiveness in asthmatic mice

Effects of increasing sensitizing doses of ovalbumin on airway hyperresponsiveness in asthmatic mice

Evaluation of Analgesic, Antioxidant, Cytotoxic and Thrombolytic Potential of Stachytarpheta cayennensis (Rich.) Vahl Leaves

In silico analysis of plants biomass phytochemicals against β2 adrenergic receptor

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