Anti-apoptotic effect of trans-resveratrol on paclitaxel-induced apoptosis in the human neuroblastoma SH-SY5Y cell line

Nicolini, Gabriella; Rigolio, R; Miloso, Mariarosaria; Bertelli, A.; Tredici, G

doi:10.1016/s0304-3940(01)01654-8

Cited by 78 publications

(57 citation statements)

References 13 publications

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“…Interestingly, a different effect of resveratrol on the taxol activity was observed in the human neuroblastoma cell line SH-SY5Y. 98 In this case, the compound prevents the apoptotic activity of taxol.…”

Section: © 2 0 0 7 L a N D E S B I O S C I E N C E D O N O T D I S mentioning

confidence: 99%

Resveratrol: From Basic Science to the Clinic

Cucciolla¹,

Borriello²,

Oliva³

et al. 2007

Cell Cycle

158

102

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Plants produce an extraordinary array of low molecular mass natural products endowed with biological activity. Among these molecules, resveratrol (3,5,4'-trihydroxystilbene) has been identified as an inhibitor of carcinogenesis with a pleiotropic mode of action. Extensive literature on its anticancer activity, performed in cellular models, suggests a potential antiproliferative and apoptogenic use of the stilbene. Similarly, studies on implanted cancers and chemical-induced tumors confirm a potential chemotherapeutical interest of the compound. Moreover, recent intriguing studies have demonstrated, in mice, that the negative effects (insulin resistance and hyperglycemia) of a high-fat diet might be prevented by resveratrol treatment. Despite these promising observations, only few clinical trials have been performed on the compound due to the scarce interest of pharmaceutical industry. We suggest that resveratrol might be considered an interesting anticancer compound in association with more specific target-oriented drugs.

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Section: © 2 0 0 7 L a N D E S B I O S C I E N C E D O N O T D I S mentioning

confidence: 99%

Resveratrol: From Basic Science to the Clinic

Cucciolla¹,

Borriello²,

Oliva³

et al. 2007

Cell Cycle

158

102

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“…In the same study by Li et al, (2000), the maximal effect of RVT on BK type K+ channels was determined at 100 μM. Since 100 μM RVT was found effective in a comparable study in organotypic brain slice cultures for determining RVT neuroprotective or anti-apoptotic effects in organotypic brain slice cultures (Raval et al, 2006;Nicolini et al, 2001), we therefore used the same concentration of RVT in our study. 0.1% DMSO was also included in control solutions to rule out any potential effects of DMSO alone.…”

Section: Solutions and Reagentsmentioning

confidence: 99%

Resveratrol attenuates early pyramidal neuron excitability impairment and death in acute rat hippocampal slices caused by oxygen-glucose deprivation

Zhang

Schools²,

Lei

et al. 2008

Experimental Neurology

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Accumulating evidence indicates that the polyphenol resveratrol (trans-3, 5, 4′-trihydroxystibene, RVT) potently protects against cerebral ischemia neuronal damage due to its oxygen free radicals scavenging and antioxidant properties. However, it is unknown whether RVT can attenuate ischemiainduced early impairment of neuronal excitability. To address this question, we simulated ischemic conditions by applying oxygen-glucose deprivation (OGD) to acute rat hippocampal slices and examined the effect of RVT on OGD-induced pyramidal neuron excitability impairment using wholecell patch clamp recording. 100 μM RVT largely inhibited the 15 min OGD-induced progressive membrane potential (Vm) depolarization and the reduction in evoked action potential frequency and amplitude in pyramidal neurons. In the parallel neuronal viability study using TO-PRO-3 iodide staining, 20 min OGD induced irreversible CA1 pyramidal neuronal death which was significantly reduced by 100 μM RVT. No similar effects were found with PQQ treatment, an antioxidant also showing potent neuroprotection in the rat rMCAO ischemia model. This suggests that antioxidant action per se, is unlikely accounting for the observed early effects of RVT. RVT also markedly reduced the frequency and amplitude of AMPA mediated spontaneous excitatory postsynaptic currents (sEPSCs) in pyramidal neurons, which is also an early consequence of OGD. RVT effects on neuronal excitability were inhibited by the large conductance potassium channel (BK channel) inhibitor paxilline. Together, these studies demonstrate that RVT attenuates OGD induced neuronal impairment occurring early in the simulated ischemia slice model by enhancing the activation of BK channel and reducing the OGD-enhanced AMPA/NMDA receptor mediated neuronal EPSCs. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. NIH Public Access INTRODUTIONRecent studies have shown that resveratrol (RVT) can protect ischemia damage in a variety of organs, such as brain, kidney and heart (Ray, et al., 1999; Bastianetto et al., 2000;Chander and Chopra, 2006). RVT is one of the primary active polyphenol compounds identified in red wine (Jang et al., 1997; Bastianetto et al., 2000;Oak et al., 2005), which has attracted increasing research interest on how this compound could offer such a miracle protection. Mechanistically, RVT acts as a potent antioxidant and scavenger of hydroperoxyl free radicals (Pace-Asciak et al., 1996; Mokni et al., 2006;Das et al., 2006). These protective actions of RVT in cerebral ischemia have so far been identified from studies in vivo and in cultured n...

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“…Neurotoxicity was evaluated by the capacity of these compounds to induce apoptosis (10,15,22). We have previously reported that CDDP may protect human neuroblastoma SH-SY5Y cells from paclitaxel-induced apoptosis, whereas low doses of paclitaxel do not protect from CDDP neurotoxicity (23). In the present study, we investigated the possible mechanisms of protection of low-dose CDDP, analyzing the modulation of some intracellular pathways known to be altered in paclitaxel-induced apoptosis (15).…”

Section: Introductionmentioning

confidence: 92%

“…This cell line is a reliable model for studying the neurotoxic effect of platinum compounds or taxanes (20) and for elucidating the mechanisms of diabetic neuropathy (21). Neurotoxicity was evaluated by the capacity of these compounds to induce apoptosis (10,15,22). We have previously reported that CDDP may protect human neuroblastoma SH-SY5Y cells from paclitaxel-induced apoptosis, whereas low doses of paclitaxel do not protect from CDDP neurotoxicity (23).…”

Section: Introductionmentioning

confidence: 99%

Low-dose cisplatin protects human neuroblastoma SH-SY5Y cells from paclitaxel-induced apoptosis

Villa¹,

Miloso²,

Nicolini³

et al. 2005

Molecular Cancer Therapeutics

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Combined anticancer therapy using platinum compounds and antitubulins has increased the risk of neurotoxicity. However, the combination of low-dose cisplatin (CDDP) with toxic doses of paclitaxel significantly reduces cellular death in a human neuroblastoma SH-SY5Y cell line. To analyze the mechanisms of this protection, we evaluated various signaling molecules possibly involved in apoptosis and some relevant cell cycle regulatory proteins. CDDP does not interfere with the tubulin-stabilizing action of paclitaxel. The evaluation of molecular pathways involved in apoptosis indicates that the Bcl-2 but not the caspases may be involved in the CDDP protection of paclitaxelinduced apoptosis. The increase in p53 protein and its nuclear accumulation suggests a possible involvement of p53 in CDDP protection. The use of the chemical inhibitor of p53, pifithrin A, excluded this possibility. The study of cyclins and the flow cytometric analysis (fluorescenceactivated cell sorting) suggest that CDDP exerts a protective action by blocking cells early in the cell cycle.

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Anti-apoptotic effect of trans-resveratrol on paclitaxel-induced apoptosis in the human neuroblastoma SH-SY5Y cell line

Cited by 78 publications

References 13 publications

Resveratrol: From Basic Science to the Clinic

Resveratrol: From Basic Science to the Clinic

Resveratrol attenuates early pyramidal neuron excitability impairment and death in acute rat hippocampal slices caused by oxygen-glucose deprivation

Low-dose cisplatin protects human neuroblastoma SH-SY5Y cells from paclitaxel-induced apoptosis

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