Anti-apoptotic Effect of Grape Seed Proanthocyanidin Extract on Cisplatin-induced Apoptosis in Rat Testis

Zhao, Yihong; Hai-lian, Zhang; Gao, Liping

doi:10.3136/fstr.21.805

Cited by 6 publications

(5 citation statements)

References 25 publications

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“…3,16,42 The balance between the pro-apoptotic and anti-apoptotic proteins was crucial for the cell to see if it will undergo apoptosis or not. 43 The CDDP treatment shifts this balance towards the pro-apoptotic signaling pathway via Bax activation and Bcl-2 degradation. 44 Immunohistochemical analysis exhibited a significant upregulation of caspase-3 protein expression in CDDP injection when compared with normal controls.…”

Section: Discussionmentioning

confidence: 99%

Candesartan in a rat model of testicular toxicity: New insight on its protective mechanism

Sherif

Sarhan

2019

Exp Biol Med (Maywood)

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Cisplatin (CDDP) is widely used as an effective chemotherapy; nevertheless, its use is associated with male reproductive system damage. Candesartan (Cand) is an angiotensin II receptor blocker which showed a protective effect against CDDP-induced testicular toxicity. This study was implemented to investigate further novel molecular protective effect of Cand. Animals were divided into four groups and treated for 10 days as: Group I (Normal control): received saline, Group II (Cand control): treated with Cand (10 mg/kg/day) orally, Group III (CDDP): injected with a single dose of 10 mg/kg CDDP intraperitoneally (ip), Group IV (Cand + CDDP): treated with Cand (10 mg/kg/day) orally plus a single ip dose of 10 mg/kg CDDP at day 3. Blood and testicular tissue collections were done at the end of the experiment. A marked decrease in testicular, body, and relative body weights in addition to testosterone level was observed in the CDDP group when compared with normal rats. In addition, exposure to CDDP showed a marked upregulation of testicular TNF-α mRNA level and a significant rise in testicular levels of total oxidant status, oxidative stress index (OSI) ratio, pro-apoptotic protein Bax, Bax/Bcl-2 ratio alongside with a marked reduction in testicular levels of total antioxidant status, antiapoptotic protein Bcl-2, and a significant upregulation of the testicular caspase-3 expression in comparison to normal group. Histopathological findings after CDDP injection showed apoptosis and necrosis in testicular tissues. Administration of Cand ameliorated these biochemical and histopathological findings. Cand exhibited a novel protective mechanism against CDDP induced-gonadal damage via antioxidant, anti-inflammatory, and anti-apoptotic activities. Impact statement Cisplatin is a commonly used drug in the treatment of solid tumors and its application is associated with testicular toxicity. The effect of candesartan in cisplatin-induced testicular toxicity and its fundamental mechanism of action were investigated. Candesartan had certainly repaired the testicular injury and ameliorated both biochemical and histopathological changes. Candesartan mitigated the gonadotoxicity induced by cisplatin via antioxidative, anti-inflammatory, and antiapoptotic actions.

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Section: Discussionmentioning

confidence: 99%

Candesartan in a rat model of testicular toxicity: New insight on its protective mechanism

Sherif

Sarhan

2019

Exp Biol Med (Maywood)

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“…The dose of GSPE used in this study was selected on the basis of the previous studies. [22][23][24][25][26][27] At the end of the experiment, the rats were sacriced under sodium pentobarbital anesthesia (60 mg kg À1 , ip). The testes and epididymis were quickly removed, cleared of adhering connective tissue, weighed and assayed immediately.…”

Section: Animals and Experimental Designmentioning

confidence: 99%

“…The protective effects of GSPE on the reproductive system have been demonstrated for various conditions. [22][23][24][25][26][27] All these studies attributes the protective effects to its antioxidant and anti-apoptotic activities. Many papers published in recent decades have documented that, the putative benecial effects of polyphenols are frequently related to their antioxidant activity.…”

Section: Introductionmentioning

confidence: 99%

Grape seed proanthocyanidin extract alleviates high-fat diet induced testicular toxicity in rats

Wang

et al. 2019

RSC Adv.

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“…The three extracts also decreased apoptosis in ECFCs, more significantly with olive extract, followed by pomace and mango. Several studies support this anti-apoptotic effect, which may be associated with its antioxidant and anti-inflammatory capacities, reflecting a protective role [47][48][49]. The reduction of the apoptotic process would also support differentiation outcomes at the expense of a decrease in proliferating cells.…”

Section: Discussionmentioning

confidence: 88%

Pro-Angiogenic Effects of Natural Antioxidants Extracted from Mango Leaf, Olive Leaf and Red Grape Pomace over Endothelial Colony-Forming Cells

et al. 2022

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Cardiovascular diseases remain the leading cause of death worldwide, mainly triggered by the formation of atherosclerotic plaques that reduce blood flow. Angiogenic cell therapy based on endothelial colony forming cells (ECFCs) constitutes a promising alternative to promote vascular revascularization; however, under the oxidative environment that prevails in ischemic areas, these cells become impaired. Thus, it is necessary to investigate strategies to enhance their regenerative properties. Antioxidant substances, such as polyphenols, have been shown to be useful for this purpose. In the current study we evaluated the potential of mango leaves, olive leaves and red grape pomace extracts, rich in polyphenols, to promote ECFC reparative effects. For this, aqueous and ethanolic extracts of the aforementioned raw materials were obtained by pressurized liquid extraction (PLE). After evaluating the polyphenol content and the antioxidant activity, in vitro assays were carried out, and we found that ethanolic extracts at low concentrations improved angiogenic capacities of ECFCs and reduced proliferation, apoptosis, and the inflammatory response of these cells. Overall, mango leaves ethanolic extract provided the most promising results, but all three extracts ameliorated the functionality of ECFCs.

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Anti-apoptotic Effect of Grape Seed Proanthocyanidin Extract on Cisplatin-induced Apoptosis in Rat Testis

Cited by 6 publications

References 25 publications

Candesartan in a rat model of testicular toxicity: New insight on its protective mechanism

Candesartan in a rat model of testicular toxicity: New insight on its protective mechanism

Grape seed proanthocyanidin extract alleviates high-fat diet induced testicular toxicity in rats

Pro-Angiogenic Effects of Natural Antioxidants Extracted from Mango Leaf, Olive Leaf and Red Grape Pomace over Endothelial Colony-Forming Cells

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