Anti-angiogenic function of a Korean Ginseng and Toad venom complex, Doksamsumsu-dan (DSSSD) analyzed by a forwarded phase antibody microarray

Kang, H.; Park, Byung-Rok; Yoo, Hwa‐Seung; Kwon, Ki-Rok; Kang, In‐Cheol

doi:10.1007/s13206-015-9308-6

Cited by 9 publications

(1 citation statement)

References 27 publications

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“…1 Due to the multiplex performance, microarray technologies have been widely utilized in biological fields such as genetics, 2 protein-protein interaction study, 3 antibody screening, 4 immunoassays, 5 and food research. 6 In general, microarray-based assays have been performed by soaking the array substrate in a sample solution. 7 However, a depletion problem of free target molecules near the reaction surface of the microarray occurs, which is intensified as target molecules are captured by the immobilized receptors because the mass transport of target molecules depends on only diffusion under the static environment, resulting in poor reaction efficiency.…”

Section: Introductionmentioning

confidence: 99%

Microarray-integrated optoelectrofluidic immunoassay system

Han

Park

2016

Biomicrofluidics

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A microarray-based analytical platform has been utilized as a powerful tool in biological assay fields. However, an analyte depletion problem due to the slow mass transport based on molecular diffusion causes low reaction efficiency, resulting in a limitation for practical applications. This paper presents a novel method to improve the efficiency of microarray-based immunoassay via an optically induced electrokinetic phenomenon by integrating an optoelectrofluidic device with a conventional glass slide-based microarray format. A sample droplet was loaded between the microarray slide and the optoelectrofluidic device on which a photoconductive layer was deposited. Under the application of an AC voltage, optically induced AC electroosmotic flows caused by a microarray-patterned light actively enhanced the mass transport of target molecules at the multiple assay spots of the microarray simultaneously, which reduced tedious reaction time from more than 30 min to 10 min. Based on this enhancing effect, a heterogeneous immunoassay with a tiny volume of sample (5 ll) was successfully performed in the microarrayintegrated optoelectrofluidic system using immunoglobulin G (IgG) and anti-IgG, resulting in improved efficiency compared to the static environment. Furthermore, the application of multiplex assays was also demonstrated by multiple protein detection. Published by AIP Publishing. [http://dx

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