Anti-angiogenic effects of theophylline on developing zebrafish (Danio rerio) embryos

Bakkiyanathan, Antony; Nathan, Jhansi; Ravikumar, Swapna; Gopalakrishnan, Thamarai Selvi; Aruldas, Felicia Mary Michael; Malathi, R.

doi:10.1016/j.bionut.2012.03.001

Cited by 17 publications

(12 citation statements)

References 13 publications

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“…Zebrafish model system has gained much attention in recent years as an in vivo model for angiogenesis study due to its physiological similarities with mammals. 24,25 The results of this study showed that the aqueous extract of M. koenigii leaves are quite safe and the deleterious effects on development are visible only at high concentrations of 1,500 ppm and above. The developmental defects such as edema in heart, incomplete yolk sac absorption, and absence of swim bladder were observed in embryos when exposed to 2,000 ppm and above of M. koenigii extracts.…”

Section: Discussionmentioning

confidence: 74%

Evaluation of Toxicity and Antiangiogenic Activity of Murraya koenigii Leaf Extracts in Zebrafish

Dinesh

Nanjappa

Babu

et al. 2020

Journal of Health and Allied Sciences NU

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Introduction This study aimed to evaluate the toxicity and the antiangiogenic activity of Murraya koenigii leaf extracts in zebrafish. Materials and Methods Crude aqueous and ethanol extracts of M. koenigii leaves were obtained following a standard solvent-based extraction method. The toxicity of these extracts on zebrafish embryonic development was determined and the LC50 values were calculated. Finally, the antiangiogenic activity of the M. koenigii leaf extracts was evaluated in a transgenic zebrafish (Tg [fli1: GFP]) that express GFP under the control of fli1, a transcription factor involved in vasculogenesis. Results The LC50 value of the aqueous extract of M. koenigii leaves was much larger (2,450 ppm) compared with that of ethanol extract (9.85 ppm), suggesting a lower toxicity of the aqueous extract. The antiangiogenic activity was evident only in aqueous extract, with significant effects in formation of intersegmental vessels and subintestinal veins in treated Tg (fli1: GFP) zebrafish embryos. Conclusion This study demonstrated the biological activity of M. koenigii leaf extracts as angiogenesis suppressor, suggesting its therapeutic potential as an angiogenesis inhibitor. Identification of active components from natural sources with potent antiangiogenic activity could lead to the development of anticancer agents.

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Section: Discussionmentioning

confidence: 74%

Evaluation of Toxicity and Antiangiogenic Activity of Murraya koenigii Leaf Extracts in Zebrafish

Dinesh

Nanjappa

Babu

et al. 2020

Journal of Health and Allied Sciences NU

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“…The use of zebrafish as an in vivo angiogenesis model has gained much attention due to its physiological similarities to mammals. 55 , 56 We were able to show that girinimbine (20 μg/mL) effectively inhibited angiogenesis in zebrafish embryos following 24 hour exposure ( Figure 7B ). Exposure to higher concentrations of girinimbine did not result in toxicity to the embryos, and 20 μg/mL was the minimum concentration of girinimbine to effectively inhibit ISV formation.…”

Section: Discussionmentioning

confidence: 91%

In vitro and in vivo anti-angiogenic activity of girinimbine isolated from Murraya koenigii

Iman¹,

Karimian²,

Mohan³

et al. 2015

DDDT

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Girinimbine is a carbazole alkaloid isolated from the stem bark and root of Murraya koenigii. Here we report that girinimbine is an inhibitor of angiogenic activity both in vitro and in vivo. MTT results showed that girinimbine inhibited proliferation of human umbilical vein endothelial cells, while results from endothelial cell invasion, migration, tube formation, and wound healing assays demonstrated significant time- and dose-dependent inhibition by girinimbine. A proteome profiler array done on girinimbine-treated human umbilical vein endothelial cells showed that girinimbine had mediated regulation of pro-angiogenic and anti-angiogenic proteins. The anti-angiogenic potential of girinimbine was also evidenced in vivo in the zebrafish embryo model wherein girinimbine inhibited neo vessel formation in zebrafish embryos following 24 hours of exposure. Together, these results showed that girinimbine could effectively suppress angiogenesis, suggestive of its therapeutic potential as a novel angiogenesis inhibitor.

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“…Each well in microtitre plate is filled with 100μl of embryo medium suspended with drug solutions (blank, test and standard) in different range of concentrations (10-100 μg/ml). Embryos were transferred using a dropper to the microtitre plate, one embryo per well 14,15 .…”

Section: Materials and Methods: Preparation Of Extractmentioning

confidence: 99%

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2015

IJPSR

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Introduction:Angiogenesis is the formation of new blood vessels which is a process involved in development of tumors and other neovascular diseases. Developmental angiogenesis in zebra fish embryos is a relevant and emergent model to evaluate the anti-angiogenesis activity which was used in present study. With this model, blood vessel formation and the blood flow can be clearly observed. Various parts of Neem tree were proved to have potential anticancer activity. Based on this fact, neem root has been selected for this study. Method: Zebra fish embryos were treated with different concentrations of Water soluble fractions of crude methanolic extract of neem root, Imatinib (standard) and control. Then, they were observed for 3 days within 24 hour interval. Results: Various phenotypical abnormalities that were observed during the development of larvae had been captured using digital microscope. Toxicity and survival rates were noted. Conclusion: Based on the results obtained, the water soluble fractions of methanolic extract of neem root was found to have the ability to inhibit in -vivo angiogenesis. Therefore, it can be concluded that, the study drug have the tumor growth inhibition properties.

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Anti-angiogenic effects of theophylline on developing zebrafish (Danio rerio) embryos

Cited by 17 publications

References 13 publications

Evaluation of Toxicity and Antiangiogenic Activity of Murraya koenigii Leaf Extracts in Zebrafish

Evaluation of Toxicity and Antiangiogenic Activity of Murraya koenigii Leaf Extracts in Zebrafish

In vitro and in vivo anti-angiogenic activity of girinimbine isolated from Murraya koenigii

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