Anti-angiogenic effect of a Palladium(II)-Saccharinate Complex of Terpyridine in vitro and in vivo

Armutak, Elif Ilkay; Gürel-Gürevin, Ebru; Kıyan, Hülya Tuba; Aydınlık, Şeyma; Yılmaz, Veysel T.; Dimas, Konstantinos; Ulukaya, Engin

doi:10.1016/j.mvr.2016.09.002

Cited by 11 publications

(13 citation statements)

References 33 publications

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“…It is well established that HUVECs are organized into lumen-like structures when cultured on Matrigel (23,(28)(29)(30). Therefore, we further examined the anti-angiogenic effects of doxorubicin, chloroquine and their combination on tube formation in HUVECs.…”

Section: Discussionmentioning

confidence: 99%

“…The in vivo CAM assay was performed as described previously by our research group (23). Fertilized hen eggs previously incubated for 72 h at 36.5˚C and at a relative humidity of 80% were positioned in a horizontal position and rotated several times.…”

Section: Methodsmentioning

confidence: 99%

“…Anti-angiogenic effects were evaluated by using a scoring system (see Table I) (23,24). The anti-angiogenic scores were calculated using the following formula: Average score=[Number of eggs (score >1)×2+number of eggs (score >0.75-1)×1]/(total number of eggs scored).…”

Section: Methodsmentioning

confidence: 99%

“…Importantly, doxorubicin and Table I. Semi-quantitative scoring system of anti-angiogenic effects on chick embryo chorioallantoic membrane (CAM) after treatment (23,24). chloroquine combination did not lead to membrane irritation nor toxicity at the tested concentration.…”

Section: Anti-angiogenic Effects Of Doxorubicin Chloroquine and Theimentioning

confidence: 99%

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Chloroquine Used in Combination with Chemotherapy Synergistically Suppresses Growth and Angiogenesis In Vitro and In Vivo

et al. 2018

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Anti-angiogenic Effects Of Doxorubicin Chloroquine and Theimentioning

confidence: 99%

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Chloroquine Used in Combination with Chemotherapy Synergistically Suppresses Growth and Angiogenesis In Vitro and In Vivo

et al. 2018

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“…In the literature, there are described terpyridine complexes containing platinum, ruthenium, vanadium, palladium, copper, iron, and others metals, which exhibit biological activity. What is worth to emphasizing, the knowledge about the activity of free ligands based on terpyridine structure is relatively unknown…”

Section: Introductionmentioning

confidence: 99%

4′‐Phenyl‐2,2′:6′,2′′‐terpyridine Derivatives Containing 1‐Substituted‐2,3‐Triazole Ring: Synthesis, Characterization and Anticancer Activity

et al. 2018

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Terpyridine moiety is known for chelating and interesting physicochemical properties enabling some applications. Its biological activity, particularly anticancer potency is not well studied. Herein we present six 4′‐(1‐substituted‐2,3‐triazol‐4‐yl)phenyl‐2,2′:6′,2′′‐terpyridine derivatives with glycidyl, cyclohexylmethyl, benzyl, 4‐tert‐butylbenzyl, pentafluorobenzyl, and 2,6‐dichlorobenzyl groups which were obtained via Cu(I)‐catalysed 1,3‐dipolar cycloaddition reaction. The photophysical such as optical (absorption and photoluminescence spectra, quantum yields and lifetimes) and thermal (by using differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA)) properties were examined. In addition, theoretical studies (DFT and TD‐DFT) were performed to provide a deeper understanding of the experimental results. In vitro studies on the obtained novel structures were conducted with selected series of cell lines to check their bioactivity and toxicity. The most active compound reached a nanomolar level with good selectivity index better than doxorubicin. Mechanism of action for these terpyridines was also investigated which suggest intercalation of DNA as a trigger of cell death.

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Pd(II) and Pt(II) saccharinate complexes with two phosphine derivatives: Synthesis, anticancer and antiangiogenic activities

Icsel,

Yilmaz,

Aygun

et al. 2024

Applied Organom Chemis

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As clinically used anticancer Pt(II) drugs have severe side effects, there is a growing interest for new metal complexes with great potential for cancer therapy. The current work aimed to prepare and characterize new Pd(II) and Pt(II) saccharinate (sac) complexes bearing pyridyl‐ and benzyldiphenylphosphines (PPh2Py and PPh2Bz, respectively), cis‐[Pd(sac)2(PPh2Py)2] (1), cis‐[PtCl(sac)(PPh2Py)2]·0.5DMF (2), cis‐[Pd(sac)2(PPh2Bz)2]·DMF (3) and trans‐[PtCl(sac)(PPh2Bz)2] (4) as promising anticancer and antiangiogenic drugs. The anticancer activity of the complexes was screened against seven cancer cell lines including HCT116 (colon), HepG2 (liver), MDA‐MB‐231 (breast), PANC‐1 (pancreatic), A549 (lung), C6 (glioma), DU145 (prostate) and normal human lung epithelial cells (BEAS‐2B). 1 and 2 did not show biological activity below 20 μM at 48 h, whereas 3 and 4 displayed significant cytotoxic effect on the cancer cells. 4 was the most potent complex (IC50 = 2.2–12.1 μM) and displayed much greater cytotoxicity than cisplatin in all the cancer cell lines. 4 caused apoptosis in HCT116 cells as evidenced by annexin V positivity and caspase 3/7 activity assays. Furthermore, the inhibition of antiapoptotic Bcl‐2 proteins by the complex suggested the intrinsic apoptosis. In addition, 4 greatly enhanced generation of intracellular reactive oxygen species (ROS) and consequently caused remarkable DNA double‐strand breaks in HCT116 cells. Moreover, the chick chorioallantoic membrane (CAM) assay was used to evaluate antiangiogenic potential of 4. The complex effectively inhibited angiogenesis at a dose of 50 ng, suggesting it as a promising multi‐targeted agent for antiangiogenic cancer treatment.

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Anti-angiogenic effect of a Palladium(II)-Saccharinate Complex of Terpyridine in vitro and in vivo

Cited by 11 publications

References 33 publications

Chloroquine Used in Combination with Chemotherapy Synergistically Suppresses Growth and Angiogenesis In Vitro and In Vivo

Chloroquine Used in Combination with Chemotherapy Synergistically Suppresses Growth and Angiogenesis In Vitro and In Vivo

4′‐Phenyl‐2,2′:6′,2′′‐terpyridine Derivatives Containing 1‐Substituted‐2,3‐Triazole Ring: Synthesis, Characterization and Anticancer Activity

Pd(II) and Pt(II) saccharinate complexes with two phosphine derivatives: Synthesis, anticancer and antiangiogenic activities

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