Anti-Angiogenesis Therapy of Cancer Cells using 153Sm-Bevasesomab

Abstract

Purpose: Angiogenesis is essential for tumor growth or metastasis. Avastin is a monoclonal antibody that is used in treating angiogenesis. We labelled this monoclonal antibody with samarium153 and performed in vitro quality control tests as a first step in the production of a new radiopharmaceutical.Material and Methods: For a successful radiolabeling, we chose DOTA-NHS as the bifunctional chelating agent and optimized radiolabeling condition with modifications of the factors such as reaction time and molar ratio which are known to be very critical in radiolabeling. The efficiency and in vitro stability of antibody labelling were determined using thin layer chromatography. The integrity of the radiolabeled antibody was checked by SDS-PAGE. Biodistribution study of 153Sm-DOTA -avastin was carried out in BALB/c mice at 2, 24, 48 and 72 hours after injection. Immunoreactivity and toxicity of the complex were tested on colon cancer cell line by MTT.Results: The efficiency of antibody labelling was more than 99%. The in vitro stability of the labelled product in human serum after 120h was 78 ±2%. There was no fragmentation in the labelled antibody during SDS-PAGE protocol. The highest of %ID/g was observed in the blood, liver, lungs and spleen. The immunoreactivity of the complex was 89±1.4%. At a concentration of 1 nM, the complex killed 70±3% of SW480 cells. At 1.9 nM, 90±5% of the cells were killed.Discussion: The monoclonal antibody avastin against angiogenesis was effectively radiolabeled with 153Sm. The results showed that the new complex could be considered a promising tracer for noninvasive delineation of angiogenesis.

Identification of novel biomarkers and small-molecule compounds for nasopharyngeal carcinoma with metastasis

“…Chemotherapy plays an important role in the treatment of cancer metastasis. [ 43 , 44 ] We identified a few small molecules that hold promise of therapeutic efficacy against NPC metastasis. These small molecules including sanguinarine, verteporfin, and chrysin, have been reported to display anti-metastatic activity.…”

The occult nodal metastasis rate of early tongue cancer (T1–T2)

“…However, recent studies report promising methods that improve diagnostic accuracies in detecting tumor metastasis. In 2018, Yavari [ 43 ] reported an effective labeling of tumor angiogenesis using monoclonal antibody avastin with 153Sm. Also, anthropomorphic phantoms of the head and neck can be used for CT scans, which reduce radiation risk while maintaining image quality.…”

Clinicopathological significance of VEGF and pAkt expressions in oral squamous cell carcinoma

“…Previous studies have confirmed that over-expression of VEGF is linked with poor survival in cancer patients including ovarian cancer, lung cancer, colorectal cancer and other types of tumours (Cheng et al 2013;Tsai et al 2013;Gkiozos et al 2015;Hou et al 2015). Anti-angiogenesis therapy is an essential step in the cancer prevention and a previous study confirmed that 153Sm-Bevasesomab and Salvia Officinalis act as anti-angiogenesis (Yavari 2018;Zare 2019). The serine/threonine kinase AKT shows critical roles in several features of cancer pathophysiology, containing cell survival, growth, metabolism as well as metastasis (Brazil et al 2004;Hennessy et al 2005;Manning and Cantley 2007).…”