Anti-androgenic activities of Ganoderma lucidum

Fujita, Rumi; Liu, Jie; Shimizu, Kuniyoshi; Konishi, Fumiko; Noda, Kiyoshi; Kumamoto, Shoichiro; Ueda, Chie; Tajiri, Hisatoshi; Kaneko, Shuhei; Suimi, Yoshitaro; Kondo, Ryuichiro

doi:10.1016/j.jep.2005.05.041

Cited by 75 publications

(45 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5,6) In addition, the treatment of the EtOH extract prepared from G. lucidum at 1.5 and 15 mg/kg/d significantly inhibited the growth of the ventral prostate induced by testosterone in rat. 6) To clarify the active principles of the EtOH extract of G. lucidum, 5a-reductase inhibitory activityguided fractionation was carried out. The inhibitory concentration leading to 50% activity loss (IC 50 ) of the EtOH extract was estimated to be 93.6 mg/ml.…”

Section: Resultsmentioning

confidence: 96%

“…The concentration of testosterone contributed by [4][5][6][7][8][9][10][11][12][13][14] C] testosterone was negligible. The sample was added to the solution at each concentration.…”

Section: Methodsmentioning

confidence: 99%

“…The radioactivity profile was determined with an imaging analyzer (FLA-5000 RF, Fuji Film Co., Ltd., Tokyo, Japan). The 5a-reductase activity was calculated from the percentage of the extent of the conversion of [4][5][6][7][8][9][10][11][12][13][14] C] testosterone to [4-14 C] dihydrotestosterone. Preparation of Methyl Ester (10) N-Methyl-N-nitrosop-toluenesulfonamide (1.25 g, 5.83 mmol) was dissolved in diethyl ether (60 ml), and was dropped into the solvent that included KOH (0.7 g, 12.5 mmol), EtOH (1.6 ml) and water (0.5 ml).…”

Section: Methodsmentioning

confidence: 99%

“…Also, the treatment of G. lucidum itself or the EtOH extract prepared from it significantly inhibited the growth of the ventral prostate induced by testosterone in rat. 5,6) In this paper, we report the isolation of the oxygenated lanostane-type triterpenoids with 5a-reductase inhibition, ganoderic acid DM (1) and 5a-lanosta-7,9(11),24-triene15a,26-dihydroxy-3-one (2) from G. lucidum and their inhibitory effects on 5a-reductase. EtOH Eextracts of Ganoderma lucidum Dried and chipped G. lucidum (15 kg) was extracted with 95% EtOH (126 l) at room temperature for 24 h by using blender.…”

mentioning

confidence: 99%

See 3 more Smart Citations

5.ALPHA.-Reductase Inhibitory Effect of Triterpenoids Isolated from Ganoderma lucidum

Liu

Kurashiki²,

Shimizu

et al. 2006

Biological & Pharmaceutical Bulletin

Self Cite

View full text Add to dashboard Cite

The microsomal enzyme steroid 5a-reductase [EC 1.3.99.5] catalyzes the NADPH-dependent reduction of D 4,5 double bond of a variety of 3-oxo-D 4 steroids.1) The principal circulating androgen is testosterone. In several androgen target tissues, like the prostate, testosterone is converted to 5a-dihydrotestosterone (DHT), which is the most potent natural androgen. This process amplifies of the androgenic response, perhaps because of the higher affinity of the androgen receptor for DHT than for testosterone.2) Both 5a-reductase and DHT perform critical roles physiologically and pathologically in man. The plasma level of DHT has been reported to be elevated in patients with either benign prostatic hyperplasia (BPH) or prostatic cancer. As a result, the inhibition of 5a-reductase has become a pharmacological strategy for the treatment of BPH as well as other DHT-related disorders such as acne and male pattern baldness.3)The study of the inhibition of 5a-reductase with organic molecules has lasted more than two decades; consequently, numerous nonsteroidal and steroidal compounds have been designed and synthesized as competitive, noncompetitive, and uncompetitive inhibitors of 5a-reductase. However, it should be noted that these inhibitors have the potential to cause adverse effects such as those reported for finasteride 4) -i.e., gynecomastia, impairment of muscle growth, and severe myopathy. Hence, the emergence of therapeutic materials having fewer side effects-preferably, edible natural products-would be highly desirable if their safety could be guaranteed.For thousands of years, mushrooms have been known as a source of medicine. In our previous screening of mushrooms, we discovered that the EtOH extract of Ganoderma lucidum (LEYSS.:FR.) KARST. (Ganodermataceae) (Fig. 1) showed the strongest 5a-reductase inhibitory activity. Also, the treatment of G. lucidum itself or the EtOH extract prepared from it significantly inhibited the growth of the ventral prostate induced by testosterone in rat. 5,6) In this paper, we report the isolation of the oxygenated lanostane-type triterpenoids with 5a-reductase inhibition, ganoderic acid DM (1) and 5a-lanosta-7,9(11),24-triene15a,26-dihydroxy-3-one (2) from G. lucidum and their inhibitory effects on 5a-reductase. EtOH Eextracts of Ganoderma lucidum Dried and chipped G. lucidum (15 kg) was extracted with 95% EtOH (126 l) at room temperature for 24 h by using blender. The extracts were filtered through ADVANTEC No. 2 filter paper, concentrated under vacuum, and then freeze-dried. The extracts (571.1 g) were stored in Ϫ20°C before assay. MATERIALS AND METHODS MaterialsDried and chipped G. lucidum (200 g) was extracted with 30% EtOH at room temperature for 24 h by using blender. The extracts were filtered through ADVANTEC No. 2 filter paper, concentrated under vacuum, and then freeze-dried. The extracts (10 g) were stored in Ϫ20°C before assay.The 95% EtOH extracts (571 g) was fractionated into three fractions 5a a-Reductase inhibitory activity-guided fractionation of the EtOH ext...

show abstract

Section: Resultsmentioning

confidence: 96%

“…The concentration of testosterone contributed by [4][5][6][7][8][9][10][11][12][13][14] C] testosterone was negligible. The sample was added to the solution at each concentration.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

5.ALPHA.-Reductase Inhibitory Effect of Triterpenoids Isolated from Ganoderma lucidum

Liu

Kurashiki²,

Shimizu

et al. 2006

Biological & Pharmaceutical Bulletin

Self Cite

View full text Add to dashboard Cite

show abstract

“…), 85 Ganoderma lucidum Fr. Krast (Ganodermataceae), 86 and other edible and medicinal mushrooms, Ayurvedic herbal mixtures, 87 or Traditional Chinese Medicine (Vaccaria, Plantago Formula, Rehmannia Eight Formula) 88,89 and other herbs. 90 Despite the popularity of saw palmetto for managing BPH in the United States and Europe, this plant has been found in an important recent study to have no beneficial effect on BPH.…”

Section: Diligencementioning

confidence: 99%

Prunus africana (Hook.f.) Kalkman: The Overexploitation of a Medicinal Plant Species and Its Legal Context

BodekerGerard

KloosterCharlotte²,

WeisbordEmma

2014

The Journal of Alternative and Complementary Medicine

View full text Add to dashboard Cite

The linkage between herbal medicines and the sustainability of medical plants from which they are manufactured is increasingly being understood and receiving attention through international accords and trade labeling systems. However, little attention is paid to the fair trade aspects of this sector, including the issue of benefit-sharing agreements with traditional societies whose knowledge and resources are being exploited for commercial herbal medicine development and production.This article examines the case of Prunus africana (Hook.f.) Kalkman, from equatorial Africa. While the conservation and cultivation dimension of the trade in P. africana has been much discussed in literature, no research appears to have focused on the traditional resource rights and related ethical dimensions of this trade in traditional medicine of Africa. Serving as a cautionary tale for the unbridled exploitation of medicinal plants, the history of P. africana extraction is considered here in the context of relevant treaties and agreements existing today. These include the Nagoya Protocol, a supplementary agreement to the Convention on Biological Diversity, the Trade-Related Aspects of Intellectual Property Rights agreement from the World Trade Organization, and two African regional frameworks: the Swakopmund Protocol and the Organisation Africaine de la Propriété Intellectuelle Initiative.In the context of strengthening medicinal plant research in Africa, a novel international capacity-building project on traditional medicines for better public health in Africa will be discussed, illustrating how access and benefit sharing principles might be incorporated in future projects on traditional medicines.

show abstract

Anti‐Androgen Effects of Extracts and Compounds from Ganoderma lucidum

Liu

Tamura

Kurashiki

et al. 2009

Chemistry & Biodiversity

Self Cite

View full text Add to dashboard Cite

The 30% EtOH extracts of Ganoderma lucidum Fr. Karst (Ganodermateceae) showed weak 5alpha-reductase inhibitory activity and binding ability to androgen receptor. When LNCaP (lymph-node carcinoma of the prostate) cells were treated with the EtOH extracts, cell proliferation was inhibited. Treatment with the extracts significantly inhibited the testosterone-induced growth of the ventral prostate in castrated rats. These results showed that G. lucidum might be a useful ingredient in the treatment of androgen-induced diseases, such as benign prostatic hyperplasia or prostate cancer. From the 30% EtOH extracts, we isolated ganoderiol F, which showed binding activity to androgen receptor and inhibited LNCaP cell proliferation, as one of the active compounds in the 30% EtOH extracts.

show abstract

Anti-androgenic activities of Ganoderma lucidum

Cited by 75 publications

References 28 publications

5.ALPHA.-Reductase Inhibitory Effect of Triterpenoids Isolated from Ganoderma lucidum

5.ALPHA.-Reductase Inhibitory Effect of Triterpenoids Isolated from Ganoderma lucidum

Prunus africana (Hook.f.) Kalkman: The Overexploitation of a Medicinal Plant Species and Its Legal Context

Anti‐Androgen Effects of Extracts and Compounds from Ganoderma lucidum

Contact Info

Product

Resources

About