Anti-amnesic activity of neferine with antioxidant and anti-inflammatory capacities, as well as inhibition of ChEs and BACE1

Jung, Hyun Ah; Jin, Seong Eun; Choi, Ran Joo; Kim, Dong Hyun; Kim, Yeong Shik; Ryu, Jong Hoon; Kim, Dong-Wook; Son, You Kyung; Park, Jin Ju; Choi, Jae Sue

doi:10.1016/j.lfs.2010.08.005

Cited by 101 publications

(63 citation statements)

References 47 publications

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“…Studies have shown that Nef can inhibit the osteosarcoma cells (Zhang et al, 2012), increase the therapeutic efficacy of anti-tumor drugs (Huang et al, 2011), exert anti-inflammatory and antioxidative effects and confer pressure-lowering effect via dilating blood vessels (Jung et al, 2010). Zhao et al (2010) found in in vitro and in vivo studies Nef could exert anti-fibrotic effect on bleomycin induced pulmonary fibrosis in a rat model via combating with inflammation and oxidation and inhibiting nuclear factor -κB.…”

Section: Discussionmentioning

confidence: 99%

Effects of neferine on TGF-β1 induced proliferation and gremlin expression in hepatic stellate cells

Li¹,

Lou²,

Wu³

et al. 2012

Bangladesh J Pharmacol

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To clarify the possible molecular mechanism underlying the anti-fibrotic effect of neferine (Nef), we investigated the in vitro effects of Nef on the proliferation of hepatic stellate cells and expressions of gremlin, TGF-1 and -SMA in these cells. TGF-1 had no influence on the proliferation of HSC-T6 cells (p>0.05) but significantly up-regulated the mRNA and protein expressions of gremlin, TGF-1 and -SMA in these cells (p<0.005). Nef could inhibit the proliferation of HSC-T6 cells in a dose and time dependent manner, and down -regulate the mRNA and protein expression of gremlin, TGF-1 and -SMA in these cells (p<0.005). Nef is an effective drug that can inhibit the proliferation of hepatic stellate cells to improve the hepatic fibrosis. Gremlin involves in the occurrence and development of hepatic fibrosis and may become a promising target in the treatment of hepatic fibrosis.

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Section: Discussionmentioning

confidence: 99%

Effects of neferine on TGF-β1 induced proliferation and gremlin expression in hepatic stellate cells

Li¹,

Lou²,

Wu³

et al. 2012

Bangladesh J Pharmacol

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“…After 24 h, the supernatants were collected and submitted to spectrophotometric analysis after Griess reaction for the detection of nitrite, which indicates NO production by RAW cells. The absorbance was measured at 540 nm (Jung et al 2010). All assays were performed three times in triplicate (n=3).…”

Section: No Production On Raw Cellsmentioning

confidence: 99%

Extraction process optimization of sulfated galactan-rich fractions from Hypnea musciformis in order to obtain antioxidant, anticoagulant, or immunomodulatory polysaccharides

et al. 2015

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The seaweed Hypnea musciformis synthesizes sulfated galactans and these galactans have been reported to have antioxidant, anticoagulant, and immunostimulatory properties. However, efficient extraction of these polysaccharides depends on the particular extraction method used, some of these polysaccharides are not extracted. Previously, we obtained galactans with different properties by using a simple extraction method. Here, we performed slight modifications to the extraction method, which led to variation in the type of polysaccharides obtained and their biological activity. We obtained four sulfated galactan-rich fractions named as FT4v, FT6v, FT8v, and FT10v. The chemical composition data showed that the fractions had high sugar and sulfate contents. Fraction FT4v exhibited the highest sulfate/sugar ratio (0.47), while FT6v (0.37), FT8v (0.39), and FT10v (0.39) presented lower sulfate/sugar ratios. Fractions FT4v and FT6v showed the highest antioxidant activity. Fraction FT4v also exhibited the greatest anticoagulant potential; however, other fractions presented marked immunomodulatory action. FT10v stimulated the highest levels of NO production whereas FT6v stimulated the highest expression of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) in RAW cells. Fraction FT10v exhibited the greatest cytotoxic action of the obtained fractions against HeLa and 786 cell lines. In vitro tests showed that the sulfated galactan-rich fractions isolated from H. musciformis with low sulfate/sugar ratios, i.e., the fractions containing fewer sulfated polysaccharides (FT6v, FT8v, and FT10v), showed marked immunostimulatory action while the fraction with the high sulfate/sugar ratio (FT4v) exhibited improved anticoagulant activity. Therefore, our results suggest that different extraction conditions promote the acquisition of sulfated galactan-rich fractions from H. musciformis with different biological activities.

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“…There are many botanicals or chemicals under biological testing [38][39][40][41][42][43][44]. Bioactive LPs modulate a neural cell differentiation, inflammation, apoptosis and intracellular trafficking in the plasma and APP metabolism of BACE1 [13,45,46].…”

Section: Discussionmentioning

confidence: 99%

Sphingosylphosphorylcholine Attenuated β–Amyloid Production by Reducing BACE1 Expression and Catalysis in PC12 Cells

Lee

et al. 2011

Neurochem Res

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Abnormal accumulation of β-amyloid (Aβ) is the main characteristic of Alzheimer's disease (AD) brain and Aβ peptides are generated from proteolytic cleavages of amyloid precursor protein (APP) by β-site APP-converting enzyme 1 (BACE1) and presenilin 1 (PS1). Sphingosylphosphorylcholine (SPC), a choline-containing sphingolipid, showed suppressive effect on Aβ production in PC12 cells which stably express Swedish mutant of amyloid precursor protein (APPsw). SPC (> 3 μM) significantly lowered the accumulation of Aβ40/42 and the expression of BACE1. However, the transcriptions of other APP processing enzymes like ADAM10 and PS1 were not affected by the SPC addition. Meanwhile, phosphocholine (PC) or other lysophospholipids, such as lysophosphatidylcholine (LPC), lysophosphatidic acid (LPA), sphingosyl-1-phosphate (S1P), did not alter BACE1 expression. Down-regulatory effect of SPC on BACE1 expression appeared to be mediated by NF-κB which is known to suppress the trans-activation of BACE1 promoter in PC12 cells. Here, the nuclear tanslocation of NF-κB was enhanced by SPC treatment in immune-fluorescent image analysis and NF-κB reporter assay. Furthermore, the catalytic activities of BACE1 and BACE2 were dose-dependently inhibited by SPC displaying IC₅₀ values of 2.79 μM and 12.05 μM, respectively. Overall, these data suggest that SPC has the potential to ameliorate Aβ pathology in neurons by down-regulating the BACE1-mediated amyloidogenic pathway.

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Anti-amnesic activity of neferine with antioxidant and anti-inflammatory capacities, as well as inhibition of ChEs and BACE1

Cited by 101 publications

References 47 publications

Effects of neferine on TGF-β1 induced proliferation and gremlin expression in hepatic stellate cells

Effects of neferine on TGF-β1 induced proliferation and gremlin expression in hepatic stellate cells

Extraction process optimization of sulfated galactan-rich fractions from Hypnea musciformis in order to obtain antioxidant, anticoagulant, or immunomodulatory polysaccharides

Sphingosylphosphorylcholine Attenuated β–Amyloid Production by Reducing BACE1 Expression and Catalysis in PC12 Cells

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