Anti‐Alzheimer's Activity of Polyphenolic Stilbene‐Rich Acetone Fraction of the Oil‐Removed Seeds of Passiflora edulis: in Vivo and in Silico Studies

Abstract

The stilbene-rich acetone fraction in high yield (6.6 %, PEAS) of Passiflora edulis Sims was prepared and evaluated for neuroprotective activity in murine Alzheimer's disease model induced by aluminum chloride and D-galactose. The phytochemical and HPLC-DAD-MS analysis of the polyphenolic stilbene-rich acetone fraction showed that it contained different stilbenes including trans-piceatannol, scirpusins AÀ B and cassigarol E. The total phenolic content (TPC) of PEAS was 413.87 � 1.71 mg GAE eqv/g. The neuroprotective activity of PEAS is typically presented in the Morris water maze-reference Spatial Memory test, where the Alzheimer's mice treated at 100 mg/kg (Alz-ED1) and 200 mg/kg (Alz-ED2) spent less than 47 % and 66 % of the time, respectively, than the Alzheimer's model mice (Alz). Two simple stilbenes, trans-piceatannol and trans-resveratrol, showed selectively inhibitory activity in silico against acetylcholinesterase (AChE). Two stilbene dimers, cassigarol E and scirpusin A, exhibited low nanomolar inhibitory potential against AChE and butyrylcholinesterase (BChE), significantly lower than those of the positive control, donepezil and tacrine. These findings suggest that the stilbenes from P. edulis seeds, particularly the stilbene dimers, warrant further investigation as potential neuroprotective candidates in the prevention of cognitive deficits associated with Alzheimer's disease.

Reusing a residue from the juice industry: extraction of oil from passion fruit seeds and its incorporation into liposomes

“…9 Some studies published in the literature show that Passiflora edulis seeds are also rich in bioactive compounds that present high antioxidant capacity 7 and anti-inflammatory, vasorelaxing, 4,[10][11][12] antitumor, antidiabetic and anti-adipogenic properties. [13][14][15] Vagula et al 16 identified phenolic compounds in Passiflora edulis seeds such as gallic acid (hydroxybenzoic acid) and chlorogenic acid (hydroxybenzoic acid).…”