Anti-allergic Effects of Extracts from Commercial Products of Cooked Burdock

Sugiura, Yoshimasa; Torii, Takayoshi; Matsuda, Kouki; Yamada, Yasuhiro

doi:10.3136/fstr.15.423

Cited by 10 publications

(10 citation statements)

References 19 publications

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“…Nordihydroguaiaretic acid, an IC 50 (the concentration of the extract required to inhibit 50% of the enzyme activity) value of 127.04 ± 8.40 μg·mL −1 , was used as a positive control. Studies carried out so far have shown that, as a raw material, burdock weakly inhibits lipoxygenase activity, with the IC 50 value of 0.99 mg·mL −1 [ 54 ]. Moreover, Chagas-Paula et al (2015) tested the ability to inhibit potato 5-lipoxygenase activity by 70% ethanolic extract from Arctium lappa leaves.…”

Section: Resultsmentioning

confidence: 99%

Arctium lappa and Arctium tomentosum, Sources of Arctii radix: Comparison of Anti-Lipoxygenase and Antioxidant Activity as well as the Chemical Composition of Extracts from Aerial Parts and from Roots

Skowrońska

Granica

Dziedzic

et al. 2021

Plants

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Arctium lappa is a weed used in traditional medicine in the treatment of skin inflammation and digestive tract diseases. Arctium tomentosum is used in folk medicine interchangeably with Arctium lappa and, according to European Medicines Agency (EMA) monography, provides an equal source of Arctii radix (Bardanae radix), despite the small amount of research confirming its activity and chemical composition. The aim of the study was the comparison of the anti-lipoxygenase and the antioxidant activity, scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide anion (O2•−), and hydrogen peroxide (H2O2), of 70 % (v/v) ethanolic extracts from the aerial parts and the roots of Arctium lappa and Arctium tomentosum. In the tested extracts, the total polyphenols content and the chemical composition, analyzed with the HPLC–DAD–MSn method, were also compared. The extracts were characterized by strong antioxidant properties, but their ability to inhibit lipoxygenase activity was rather weak. A correlation between the content of polyphenolic compounds and antioxidant activity was observed. The extracts from A. lappa plant materials scavenged reactive oxygen species more strongly than the extracts from A. tomentosum plant materials. Moreover, the extracts from A. lappa plant materials were characterized by the statistically significantly higher content of polyphenolic compounds.

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Section: Resultsmentioning

confidence: 99%

Arctium lappa and Arctium tomentosum, Sources of Arctii radix: Comparison of Anti-Lipoxygenase and Antioxidant Activity as well as the Chemical Composition of Extracts from Aerial Parts and from Roots

Skowrońska

Granica

Dziedzic

et al. 2021

Plants

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“…The amount of β-hexosaminidase release by basophilic leukemia cells (RBL-2H3) was determined using a previous method with some modifications (Sato, Shinozaki et al, 2014;Sato, Yamagami, & Tamura, 2012;Sugiura, Torii, Matsuda, & Yamada, 2009). RBL-2H3 was obtained from RIKEN BioResource Center Cell Bank (Ibaraki, Japan).…”

Section: β-Hexosaminidase Release Assay Of Rbl-2h3 Cellsmentioning

confidence: 99%

Antiallergic activities of eleven onions (Allium cepa) were attributed to quercetin 4′-glucoside using QuEChERS method and Pearson's correlation coefficient

Sato

Zhang

Yonekura

et al. 2015

Journal of Functional Foods

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“…E. stolonifera is known to produce a functional polysaccharide, alginate (Kuda et al, 2007), which inhibits HA activity (Asada et al, 1997). Interestingly, the PVPP residue did not inhibit the HA activity in by measuring the β-Hex activity as described in a previous report (Sugiura et al, 2009a). Experimental conditions for cell stimulation and exposure to tested samples was as follows.…”

Section: Removal Of Phenolic Compounds (Phlorotannins) Included In Thmentioning

confidence: 99%

“…RBL cells were cultured as described elsewhere (Sugiura et al, 2009a). Since the extent of degranulation from the mast cells is proportional to that of β-hexosaminidase (β-Hex; equivalent to histamine) (Schwartz et al, 1981), the degranulation assay by stimulated RBL cells was conducted chased from Marumo-mogami Store Co., Ltd. in Aomori Prefecture.…”

Section: Assay For Suppression Of Degranulation From Rbl-2h3 Cells Anmentioning

confidence: 99%

Inhibitory Effect of Extracts from the Brown Alga, Ecklonia stolonifera, on Enzymes Responsible for Allergic Reactions and Degranulation in RBL-2H3 Cells

Sugiura

Torii

Tanaka

et al. 2012

FSTR

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The anti-allergic effects of the edible brown alga, Ecklonia stolonifera, are known to affect both degranulation in KU812 cells and anti-inflammatory properties of RAW 264.7 cells. Additional anti-allergic effects of E. stolonifera extracts were therefore investigated by examining the inhibitory effects of algal extracts on enzymes associated with eicosanoid synthesis, the inflammation reaction, and anti-degranulation activity in rat basophilic leukemia (RBL) -2H3 cells. A methanol: chloroform (1: 2, v/v) extract of the alga inhibited the enzyme activities and degranulation in stimulated RBL cells in a dose-dependent manner. When phenolic compounds were removed from the M/C extract by treatment with polyvinylpolypyrrolidone resin, the obtained residue did not exhibit the same inhibitory activities. These results imply that the active compounds present in the M/C extract may be phenolic compounds, such as phlorotannins. In addition, the novel anti-allergic effects of E. stolonifera that contributed to inhibiting the enzymatic activities were also clarified.Keywords: degranulation, Ecklonia stolonifera, eicosanoid synthesis, inflammation, phlorotannin Abbreviations: COX: cyclooxygenase, SBL: soybean lipoxygenase, LOX: lipoxygenase, PLA 2 : phospholipase A 2 , HA: hyaluronidase, RBL: rat basophilic leukemia, EGCg: epigallocatechin gallate, PVPP: polyvinylpolypyrrolidone *To whom correspondence should be addressed. E-mail: ysugiura@fish-u.ac.jp IntroductionEcklonia stolonifera is a brown seaweed that grows along the coast of the Japan Sea (Notoya, 1995). The increased abundance of E. stolonifera populations in the last 20 years has resulted in a decrease in the abundance of Laminaria japonica, traditionally harvested for human consumption, in areas such as Aomori Prefecture in the Tohoku region (Kirihara et al., 2006). Consequently, E. stolonifera has come to be regarded as a "nuisance" species. However, from the discovery that E. stolonifera possesses anti-allergic and antiinflammatory effects by inhibiting degranulation in KU812 cells and anti-inflammation in RAW 264.7 cells (Kim et al., 2009;Shim et al., 2009), this alga could potentially be used as an anti-allergic food source.In a previous study, we found that phlorotannins (seaweed polyphenol) derived from Eisenia arborea inhibit the degranulation of cultured cells (rat basophilic leukemia (RBL) -2H3, KU812) as well as the activities of enzymes (cyclooxygenase (COX) -2, lipoxygenase (LOX), phospholipaseA 2 (PLA 2 ), and hyaluronidase (HA)) involved in allergic reactions (Sugiura et al., 2009b). Therefore, in this study, to elucidate the anti-allergic mechanisms different from those inhibiting degranulation in KU812 cells and anti-inflammation in RAW 264.7 cells, we examined inhibition of COX-2, LOX, PLA 2 and HA by the M/C extracts of the alga, as well as inhibition of degranulation in RBL-2H3 cells. To determine whether the active compounds of the M/C extract are phlorotannins, the residues of the M/C extract from which phlorotannins were removed using polyvi...

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Anti-allergic Effects of Extracts from Commercial Products of Cooked Burdock

Cited by 10 publications

References 19 publications

Arctium lappa and Arctium tomentosum, Sources of Arctii radix: Comparison of Anti-Lipoxygenase and Antioxidant Activity as well as the Chemical Composition of Extracts from Aerial Parts and from Roots

Arctium lappa and Arctium tomentosum, Sources of Arctii radix: Comparison of Anti-Lipoxygenase and Antioxidant Activity as well as the Chemical Composition of Extracts from Aerial Parts and from Roots

Antiallergic activities of eleven onions (Allium cepa) were attributed to quercetin 4′-glucoside using QuEChERS method and Pearson's correlation coefficient

Inhibitory Effect of Extracts from the Brown Alga, Ecklonia stolonifera, on Enzymes Responsible for Allergic Reactions and Degranulation in RBL-2H3 Cells

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