Anti-allergic effect of Lactobacillus helveticus SBT2171 on murine model of pollen allergy

Abstract

Background: We recently reported the suppressive effects of Lactobacillus helveticus SBT2171 (LH2171) on the proliferation and inflammatory cytokine production of primary murine immune cells in vitro. In this study, we investigated the effect of LH2171 on a murine pollen allergen- induced allergy model.Methods: Male BALB/c mice were fed a diet containing LH2171 (LH2171 group) or a diet not containing LH2171 (control group). Then, pollen allergy was induced by immunization with pollen extract emulsified in alum adjuvant and intranasal administration of pollen extract. To evaluate the anti-allergenic effect of LH2171, the antibody titer in plasma and expression of cytokines and transcription factors in immune tissues were analyzed by ELISA and qRT-PCR.Results: In the LH2171 group, the amounts of pollen-specific antibody and total IgE in plasma were significantly decreased compared with those in the control group. Proliferation and Th2 cytokine production were lower in the submandibular lymph node cells of the LH2171 group than in those of the control group. Moreover, in Peyer’s patches, the gene expression of Il10 and Foxp3 were increased in the LH2171 group.Conclusion: LH2171 has the ability to suppress production of allergy-related antibody and Th2 cytokines, which could be correlated with the induction of anti-inflammatory factors in the intestinal immune system.

Lactobacillus helveticus SBT2171 Alleviates Perennial Allergic Rhinitis in Japanese Adults by Suppressing Eosinophils: A Randomized, Double-Blind, Placebo-Controlled Study

“…In a previous study, the administration of LH2171 cells in an allergic mouse model suppressed the induction of IL5, a Th2 cytokine that induces eosinophils, in the lymph nodes [27]. Moreover, LH2171 cells suppressed the production of Th2 cytokines and induced the secretion of Th1 cytokines in spleen cells stimulated with antigen ex vivo [26].…”

“…Additionally, in a murine pollen allergen-induced allergy model, LH2171 significantly decreased the pollen-specific total antibody and total IgE levels in plasma. LH2171 suppressed the proliferation and Th2 cytokine production in the submandibular lymph node cells and increased the gene expression of IL10 and Foxp3 in Peyer's patches [27]. In addition, a randomized, double-blind, placebo-controlled study (RCT) revealed that the ingestion of fermented milk (FM) containing LH2171 daily for 12 weeks by healthy adults positive for house dust-or mite-specific antibodies improved their symptoms of ocular and nasal discomfort [28].…”

Probiotic Potential of <b><i>Lactobacillus</i></b> Species in Allergic Rhinitis

“…Significant effects of Lactobacillus strains on T reg were demonstrated in both animal and human studies [22,50,51]. For instance, Lactobacillus treatment was associated with expanded IL-10 levels [5,46,51], such as the increase in the IL-10 level about 24 ± 13% in response to intervention with L. plantarum [46]. In contrast, no associations of increased IL-10 levels and other species such as L. reuteri, L. gasseri, and L. johnsonii could be found [52].…”

“…Simon, Bern. can be drowsiness, gastrointestinal disorders, dry mouth, dizziness, headache, or infections [5]. Additionally, the window of effectiveness of these drugs is often limited by the time after the onset of an allergy.…”

“…Besides, it is important to develop a safe, lifelong therapy for the prevention of allergic reactions. In this regard, food with live and heat-killed probiotic strains would be a suitable option as both likewise alleviate symptoms of AR [5,6]. Probiotics are described as "live microorganisms" with a "health benefit on the host," when "administered in adequate Table 1.…”