Anti-allergic Effect of Bee Venom in an Allergic Rhinitis Mouse Model

Shin, Seung‐Heon; Kim, Yee-Hyuk; Kim, Jeong‐Kyu; Park, Kwan‐Kyu

doi:10.1248/bpb.b14-00102

Cited by 20 publications

(16 citation statements)

References 13 publications

(11 reference statements)

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“…Behaviour scores were measured to evaluate establishment of the AR model in mouse as previously described (Shin, Kim, Kim, & Park, 2014).…”

Section: Measurement Of Behaviour Scoresmentioning

confidence: 99%

“…From day 22 to day 30, daily intranasal instillation of 500 g OVA in 20 l PBS was performed in the treatment group, while 20 l PBS was utilized in the negative control group. Behaviour scores (number of sneezing and number of rubbing motions) were recorded on day 21, 24, 27 and 30, 15 min after OVA treatment as previously described (Shin et al, 2014), and mice were killed 24 h after treatment to withdraw blood by cardiac puncture under anaesthesia. As shown in Figure 1b,c, both sneezing times and rubbing times in 15 min were significantly increased in the AR group in a time-dependent manner.…”

Section: Establishment Of Ova-induced Ar Model In Mousementioning

confidence: 99%

“…Behaviour scores (number of sneezing and number of rubbing motions) were recorded on day 21, 24, 27 and 30,15 min after OVA treatment as previously described(Shin et al, 2014), and mice were killed 24 h after treatment to withdraw blood by cardiac puncture under anaesthesia. injection of 75 g OVA emulsified in 2 mg alum along with 200 l PBS at day 0, 7, 14 and 21.…”

mentioning

confidence: 99%

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MiR‐199‐3p–Dnmt3a–STAT3 signalling pathway in ovalbumin‐induced allergic rhinitis

Cui

Guo

Wang

et al. 2019

Experimental Physiology

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New Findings What is the central question of this study?What is the mechanism of DNA methylation in allergic rhinitis? What is the main finding and its importance?A miR‐199‐3p–Dnmt3a–STAT3 signalling pathway is involved in ovalbumin‐induced allergic rhinitis, and miR‐199‐3p antagomir can relieve the symptoms in the mouse model. Abstract Recent research has pointed out the involvement of epigenetic modifications in allergic rhinitis (AR), especially DNA methylation. However, the detailed mechanism has remained largely uncovered. We used ovalbumin (OVA) to induce AR in mouse, and behaviour scores were used to confirm its successful establishment. Histamine and other inflammatory factors were detected to further verify success of the model. Real‐time PCR was employed to identify the overexpression of miR‐199‐3p and subsequent down‐regulation of DNA methyltransferase 3a (Dnmt3a). Western blotting was utilized to detect Dnmt3a and signal transducer and activator of transcription 3 (STAT3) at the protein level. Bisulfite sequencing PCR was applied to reveal the methylation status of the Stat3 promoter region. A dual‐reporter assay was used to confirm the direct targeting of miR‐199‐3p on the Dnmt3a mRNA and an antagomir specific to miR‐199‐3p was injected to rescue the symptoms of AR. The AR model was successfully established in mouse and confirmed by both behaviour and molecular markers. We also found lowered expression of Dnmt3a and consecutive hypomethylation of Stat3 promoter and elevated expression of STAT3, which then led to overexpression of IgE and other inflammatory factors. MicroRNAs that worked on the Dnmt3a 3′‐untranslated region were predicted and then verified by dual‐reporter assay. Finally injection of a miR‐199‐3p antagomir successfully attenuated the symptoms of AR. We propose that the miR‐199‐3p–Dnmt3a–STAT3 signalling pathway is involved in OVA‐induced AR.

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“…Behaviour scores were measured to evaluate establishment of the AR model in mouse as previously described (Shin, Kim, Kim, & Park, 2014).…”

Section: Measurement Of Behaviour Scoresmentioning

confidence: 99%

Section: Establishment Of Ova-induced Ar Model In Mousementioning

confidence: 99%

mentioning

confidence: 99%

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MiR‐199‐3p–Dnmt3a–STAT3 signalling pathway in ovalbumin‐induced allergic rhinitis

Cui

Guo

Wang

et al. 2019

Experimental Physiology

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“…Animal models have demonstrated the capability of anti-inflammatory and ant-bacterial activity of BV (12). However, due to the BV has dose dependent immunosuppressive and immunostimulatory property, the determination of optimal concentration without side effect is an important for clinical application of BV.…”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory effect of bee venom in an allergic chronic rhinosinusitis mouse model

Shin

Choi

et al. 2018

Mol Med Report

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Bee venom (BV) has long been used as anti-inflammatory agent in traditional oriental medicine; however, the effect of BV on chronic rhinosinusitis (CRS) is not commonly studied. The aim of the present study was to determine the anti-inflammatory effect of BV on an allergic CRS mouse model. An allergic CRS mouse model was established following the administration of ovalbumin with Staphylococcus aureus enterotoxin B (SEB) into the nose. A total of 0.5 or 5 ng/ml of BV were intranasally applied 3 times a week for 8 weeks. Histopathological alterations were observed using hematoxylin and eosin, and Periodic acid Schiff staining. The levels of inflammatory cell infiltration, interleukin (IL)‑4, IL‑10 and interferon (INF)‑γ in nasal lavage fluid (NLF) were measured. Nuclear factor (NF)‑κB and activator protein (AP)‑1 expressions were also determined by immunohistochemical staining. The group treated with BV had significantly decreased inflammatory cell infiltration and PAS‑positive cells. The levels of INF‑γ, and neutrophil and eosinophil counts in NLF were significantly decreased, and the SEB‑induced NF‑κB and AP‑1 expressions in mouse nasal mucosa were significantly suppressed by 0.5 and 5 ng/ml BV. Thus, BV exerted significant anti‑inflammatory effects in an allergic CRS mouse model and may have potential value for the treatment of CRS.

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“…BV contains a variety of different peptides, including melittin, apamin, adolapin and mast cell degranulating (Mcd) peptide. The two main components of BV, melittin and adolapin, have anti-inflammatory effects, which involve the inhibited expression of cyclooxygenase-2 and phospholipase A2, and decreased levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-1, IL-6 and nitric oxide (23). These components are known to exert their pharmacological effects individually or interactively depending on their concentration or dose.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effects of bee venom on mast cell-mediated allergic inflammatory responses

et al. 2018

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Although bee venom (BV) is a toxin that causes bee stings to be painful, it has been widely used clinically for the treatment of certain immune‑associated diseases. BV has been used traditionally for the treatment of chronic inflammatory diseases. In this regard, the present study analyzed the effect of BV on the regulation of inflammatory mediator production by mast cells and their allergic inflammatory responses in an animal model. HMC‑1 cells were treated with BV prior to stimulation with phorbol‑12‑myristate 13‑acetate plus calcium ionophore A23187 (PMACI). The production of allergy‑associated pro‑inflammatory mediators was examined, and the underlying mechanisms were investigated. Furthermore, to investigate whether BV exhibits anti‑inflammatory effects associated with anti‑allergic effects in vivo, a compound 48/80‑induced anaphylaxis model was used. BV inhibited histamine release, mRNA expression and production of cytokines in the PMACI‑stimulated HMC‑1 cells. Furthermore, the inhibitory effects of BV on mitogen‑activated protein kinase (MAPK), MAPK kinase, signal transducer and activator of transcription 3 (STAT3) and Akt were demonstrated. The present study also investigated the ability of BV to inhibit compound 48/80‑induced systemic anaphylaxis in vivo. BV protected the mice against compound 48/80‑induced anaphylactic‑associated mortality. Furthermore, BV suppressed the mRNA expression levels of pro‑inflammatory cytokines, and suppressed the activation of MAPK and STAT3 in this model. These results provide novel insights into the possible role of BV as a modulator for mast cell‑mediated allergic inflammatory disorders.

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Anti-allergic Effect of Bee Venom in an Allergic Rhinitis Mouse Model

Cited by 20 publications

References 13 publications

MiR‐199‐3p–Dnmt3a–STAT3 signalling pathway in ovalbumin‐induced allergic rhinitis

MiR‐199‐3p–Dnmt3a–STAT3 signalling pathway in ovalbumin‐induced allergic rhinitis

Anti-inflammatory effect of bee venom in an allergic chronic rhinosinusitis mouse model

Inhibitory effects of bee venom on mast cell-mediated allergic inflammatory responses

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