Anti-Allergic Compounds from the Deep-Sea-Derived Actinomycete Nesterenkonia flava MCCC 1K00610

Abstract

A novel cyclic ether, nesterenkoniane (1), was isolated from the deep-sea-derived actinomycete Nesterenkonia flava MCCC 1K00610, together with 12 known compounds, including two macrolides (2, 3), two diketopiperazines (4, 5), two nucleosides (6, 7), two indoles (8, 9), three phenolics (10–12), and one butanol derivate (13). Their structures were established mainly on detailed analysis of the NMR and MS spectroscopic data. All 13 compounds were tested for anti-allergic activities using immunoglobulin E (IgE) mediated rat mast RBL-2H3 cell model. Under the concentration of 20 μg/mL, 1 exhibited moderate anti-allergic activity with inhibition rate of 9.86%, compared to that of 37.41% of the positive control, loratadine. While cyclo(d)-Pro-(d)-Leu (4) and indol-3-carbaldehyde (8) showed the most potent effects with the IC50 values of 69.95 and 57.12 μg/mL, respectively, which was comparable to that of loratadine (IC50 = 35.01 μg/mL). To the best of our knowledge, it is the first report on secondary metabolites from the genus of Nesterenkonia.

Recovery and resiliency of skin microbial communities on the southern leopard frog (Lithobates sphenocephalus) following two biotic disturbances

“…Of 13 metabolites characterized from Nesterenkonia spp. [41], some have antifungal activity including Cyclo(d)-Pro-(D)-Leu [42], 2,3-butanediol [43]. The presence of some core OTUs in the community may play a foundational role, producing antifungal compounds or facilitating the growth of other microorganisms that do.…”

Microindolinone A, a Novel 4,5,6,7-Tetrahydroindole, from the Deep-Sea-Derived Actinomycete Microbacterium sp. MCCC 1A11207

“…The anti-allergic activity, indexed by the β-hexosaminidase release, was measured for the efficiency of the RBL-2H3 cell degranulation inhibition rate using IgE-mediated mast cell allergic reaction [ 8 , 35 ]. In short, RBL-2H3 cells were seeded into 96-well cell culture plates (1 × 10 5 cells/well) to incubate with dinitrophenol (DNP) specific IgE overnight.…”

“…However, only four compounds were reported from this genus [ 6 , 7 ]. In our current research for novel compounds from deep-sea-derived microorganisms [ 8 , 9 , 10 ], the actinomycete Microbacterium sp. MCCC 1A11207, isolated from southwestern Indian Ocean sediment, was subjected to a systematic chemical examination.…”

Microindolinone A, a Novel 4,5,6,7-Tetrahydroindole, from the Deep-Sea-Derived Actinomycete <em>Microbacterium </em>sp. MCCC 1A11207

“…The anti-allergic activity, indexed by the β-hexosaminidase release, was measured for the efficiency of the RBL-2H3 cell degranulation inhibition rate using IgE-mediated mast cell allergic reaction [8,35]. In short, RBL-2H3 cells were seeded into 96-well cell culture plates (1×10 5 cells/well) to incubate with DNP-specific IgE overnight.…”

“…However, only four compounds were obtained from this genus [6,7]. In our current research for structurally novel secondary metabolites from deep-sea-derived microorganisms [8][9][10], the actinomycete Microbacterium amylolyticum MCCC 1A11207, isolated from southwestern Indian Ocean sediment, was subjected to a systematic chemical examination. Consequently, one new and 18 known compounds were obtained.…”