Anti-Aging Effects of Leontopodium alpinum (Edelweiss) Callus Culture Extract through Transcriptome Profiling

Abstract

Edelweiss (Leontopodium Alpinum) in the family Asteraceae is a wildflower that grows in rocky limestone places. Here, we investigated the efficacy of edelweiss callus culture extract (Leontopodium Alpinum callus culture extract; LACCE) using multiple assays from in vitro to in vivo as well as transcriptome profiling. Several in vitro assay results showed the strong antioxidant activity of LACCE in response to UVB treatment. Moreover, LACCE suppressed inflammation and wrinkling; however, moisturizing activity was increased by LACCE. The clinical test in vivo demonstrated that constant application of LACCE on the face and skin tissues improved anti-periorbital wrinkles, skin elasticity, dermal density, and skin thickness compared with the placebo. The RNA-Sequencing results showed at least 16.56% of human genes were expressed in keratinocyte cells. LACCE up-regulated genes encoding several KRT proteins; DDIT4, BNIP3, and IGFBP3 were involved in the positive regulation of the developmental process, programmed cell death, keratinization, and cornification forming skin barriers, which provide many advantages in the human skin. By contrast, down-regulated genes were stress-responsive genes, including metal, oxidation, wounding, hypoxia, and virus infection, suggesting LACCE did not cause any harmful stress on the skin. Our comprehensive study demonstrated LACCE is a promising agent for anti-aging cosmetics.

Profiling of Polyphenolic Compounds of Leontopodium alpinum Cass Callus Cultures Using UPLC/IM-HRMS and Screening of In Vitro Effects

“…Therefore, they are expected to be responsible for the effects observed in the in vitro assays that were carried out. The extract also showed strong antioxidant activity as determined through the DPPH free radical scavenging assay, in agreement with previous studies [ 13 , 16 ].…”

“…Quantitative analysis of the most abundant four phenylpropanoid derivatives specific to L. alpinum (i.e., chlorogenic acid, 3,5-dicaffeoyl quinic acid, leontopodic acids A and B) ( Table 2 ) revealed that they represent more than 57% (m/m) of the dry-frozen extract and around 15% (m/m) of the dry-frozen callus culture. The measured concentrations of these four constituents in callus culture extracts are comparable or higher than in wild plant [ 16 ] and confirm the high quantity of polyphenols obtained by the total polyphenol content determination. Therefore, they are expected to be responsible for the effects observed in the in vitro assays that were carried out.…”

“…During the last decade, the preclinical research on L. alpinum extracts effects has been focused on anti-inflammatory effect evaluation [ 14 ], leukotriene biosynthesis inhibition [ 15 ], anti-aging effects [ 16 ] and memory improving effects [ 17 ]. However, no research of edelweiss’s potential in chemoprevention has been published so far.…”

Transcriptome Profiling of Human Follicle Dermal Papilla Cells in response to Porphyra-334 Treatment by RNA-Seq

“…Of the six examined parameters of periorbital wrinkles, three (R3 (average roughness), R2 (maximum roughness), and visual assessment)) were significantly different between the treatment and control groups at both 6 and 12 weeks. Our recent study showed the improvement of periorbital wrinkles by Leontopodium Alpinum callus culture extract (LACCE) [ 32 ]. Similarly, a recent study using a cream containing low-molecular-weight heparan sulfate (LMW‐HS) and a mixture of four different naturally derived plant extracts showed the improvement of periorbital wrinkles [ 33 ].…”

Gene expression profile of human follicle dermal papilla cells in response to Camellia japonica phytoplacenta extract

“…With a plant-specific bioreactor, it is possible to generate a large quantity of specific plant cells within a short time. Thus, without mature plant cultivation, using the plant callus with a plant-specific bioreactor facilitates the production of diverse plant extracts with a quality equal to that of mature plants [18].…”