Anti-aggregation effect of carbon quantum dots on diabetogenic and beta-cell cytotoxic amylin and beta amyloid heterocomplexes

Voronova, Anna; Barras, Alexandre; Plaisance, Valérie; Pawlowski, Valérie; Boukherroub, Rabah; Abderrahmani, Amar; Szunerits, Sabine

doi:10.1039/d2nr03173f

Cited by 6 publications

(16 citation statements)

References 31 publications

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“…66 Our team provided lately valuable data to this discussion by investigating the effect of different carbon nanocolloids on hIAPP aggregation under diabetic conditions. 4 Three types of CDQs were prepared using the microwave-assisted hydrothermal method: (i) positively charged CQD-pos (ζ = +32.5 mV) 3 using glucosamine as a carbon precursor with ethylenediamine as a passivating agent, (ii) neutral CQD-neut via mixing glucose and hydrofluoric acid to catalyze dehydration reaction, 58 and (iii) negatively charged CQD-neg (ζ = −24.1 ± 1.6 mV) using glucosamine as a precursor and β-alanine as a surface passivating agent. 4 All particles were of spherical shape with an average particle size determined by TEM of 16.8 ± 6.7 nm (CQD-pos), 22.3 ± 5.7 nm (CQD-neut), and 20.8 ± 6.6 nm (CQD-neg).…”

Section: ■ Potential Of Carbon Nanoparticles and Carbon Colloids To I...mentioning

confidence: 99%

“…4 Three types of CDQs were prepared using the microwave-assisted hydrothermal method: (i) positively charged CQD-pos (ζ = +32.5 mV) 3 using glucosamine as a carbon precursor with ethylenediamine as a passivating agent, (ii) neutral CQD-neut via mixing glucose and hydrofluoric acid to catalyze dehydration reaction, 58 and (iii) negatively charged CQD-neg (ζ = −24.1 ± 1.6 mV) using glucosamine as a precursor and β-alanine as a surface passivating agent. 4 All particles were of spherical shape with an average particle size determined by TEM of 16.8 ± 6.7 nm (CQD-pos), 22.3 ± 5.7 nm (CQD-neut), and 20.8 ± 6.6 nm (CQD-neg). CQD-pos of 10 μg mL −1 slowed and reduced the amplitude of hIAPP fibrillation (Figure 7a) at high glucose levels (11 mM), but showed almost complete aggregation inhibition in the presence of a fatty acid such as palmitate.…”

Section: ■ Potential Of Carbon Nanoparticles and Carbon Colloids To I...mentioning

confidence: 99%

“…Different to carbon nanotubes, which generally exhibit higher cytotoxic effects compared to other structures within the carbon family, 71 CQDs seem to be favorable for biological applications due to their good water dispersibility and limited cytotoxicity in vitro, as shown in this Account and related literature. 3,4 In vivo toxicity is the next logical step following cellular level studies to be implemented. CQDs injected in female BALB/C mice was studied over 3 months.…”

Section: ■ Conclusion and Perspectivesmentioning

confidence: 99%

“…4,9−12 Islet β-cells express the amyloid precursor protein (APP) and the enzymatic machinery that generates the amyloidogenic Aβ42. The formed heterocomplex (hIAPP:Aβ42) is even more cytotoxic than the single oligomers 4,9 and requires the development of inhibitors that efficiently prevent the formation of homomeric and heteromeric complexes. Such inhibitors that prevent and stop β-cell dysfunction and destruction are still unmet medical dreams.…”

Section: ■ Introductionmentioning

confidence: 99%

“…The application of nanomaterials-and nanomedicine-derived approaches to limit protein aggregation in the context of T2D has been investigated by us lately and is the base for this account article. 4 While nanomedicine remains challenging, it holds great potential for the future of diabetes management, and, for the moment, the suggested benefits in diabetic care seem to outweigh the possible pitfalls. 2 Inputs from technology-driven medicine for diabetes are expected from chemistry through the synthesis of novel drug formulations, including the development of oral therapeutic protein delivery such as insulin and GLP-1 13,14 as well as alternative diabetic drug delivery routes reported by us 15−19 and others.…”

Section: ■ Introductionmentioning

confidence: 99%

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Nanoparticles and Nanocolloidal Carbon: Will They Be the Next Antidiabetic Class That Targets Fibrillation and Aggregation of Human Islet Amyloid Polypeptide in Type 2 Diabetes?

2022

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Conspectus Nanotechnology is revolutionizing human medicine. Nanoparticles (NPs) are currently used for treating various cancers, for developing vaccines, and for imaging, and other promises offered by NPs might come true soon. Due to the interplay between NPs and proteins, there is more and more evidence supporting the role of NPs for treating amyloid-based diseases. NPs can induce some conformational changes of the adsorbed protein molecules via various molecular interactions, leading to inhibition of aggregation and fibrillation of several and different amyloid proteins. Though an in depth understanding of such interactions between NPs and amyloid structures is still lacking, the inhibition of protein aggregation by NPs represents a new generation of innovative and effective medicines to combat metabolic diseases such as type 2 diabetes (T2D). Here, we lay out advances made in the field of T2D notably for optimizing protein aggregation inhibition strategies. This Account covers discussions about the current understanding of β-cells, the insulin producing cells within the pancreas, under diabetic conditions, notably increased glucose and fatty acid levels, and the implication of these conditions on the formation of human islet amyloid polypeptide (hIAPP) amylin oligomers and aggregates. Owing to the great potential of carbon nanostructures to interfere with protein aggregation, an important part of this Account will be devoted to the state of the art of therapeutic options in the form of emerging nanomaterials-based amyloidosis inhibitors. Our group has recently made some substantial progress in this regard by investigating the impact of glucose and fatty acid concentrations on hIAPP aggregation and β-cell toxicity. Furthermore, the great potential of carbon nanocolloids in reversing hIAPP aggregation under diabetic conditions will be highlighted as the approach has been validated on β-cell cultures from rats. We hope that this Account will evoke new ideas and concepts in this regard. We give some lead references below on pancreatic β-cell aspects and carbon quantum dots for managing diabetics and nanomedicine related aspects, a topic of interest in our laboratory.

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Section: ■ Potential Of Carbon Nanoparticles and Carbon Colloids To I...mentioning

confidence: 99%

Section: ■ Potential Of Carbon Nanoparticles and Carbon Colloids To I...mentioning

confidence: 99%

Section: ■ Conclusion and Perspectivesmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Nanoparticles and Nanocolloidal Carbon: Will They Be the Next Antidiabetic Class That Targets Fibrillation and Aggregation of Human Islet Amyloid Polypeptide in Type 2 Diabetes?

2022

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Functional Carbon Quantum Dots for Ocular Imaging and Therapeutic Applications

Kumar

Sher

Rencus‐Lazar

et al. 2022

Small

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functional groups such as CHO, CO, NH 2 , OH, COOH, COC, CN, NO, SH, or polymer aggregates. [11] Moreover, CDs are considered to be the most recent form of zero-dimensional carbon, with a diameter of less than 10 nm and excellent fluorescent properties. [12] These properties have led to their application in biomedical engineering [13] and sensor technology, [14,15] including heavy metal ion detection, [16] fluorescent labeling and nucleus targeted imaging of cells, [17] targeting of drug delivery for anti-tumor therapy, [18] photovoltaic devices/solar cell, [19] photocatalysts, [20] dye degradation, [21] lubricant additive, [22] supercapacitor, [23] nanoelectronic devices, [24] fuel cells, [25] anti-viral therapy, [26] photodynamic therapy, [27] gene delivery, [28] and disruption of the fibrillation of amyloidogenic peptides, such as Aβ. [29,30] Recently, Shah et al. have investigated the presence of Aβ in aging eyes and its significance in relation to Alzheimer's disease (AD) pathology. [31] They have also discussed clinical studies investigating the in vivo ocular imaging of Aβ and its relationship to brain Aβ burden, as well as therapies that target ocular Aβ. [31] Therefore, the characterization of retinal Aβ is also necessary in order to differentiate it from eye-related diseases.According to the World Health Organization, more than 2.2 billion individuals were affected by some form of visual impairment in 2020, with an estimated 35 million blind individuals. [32][33][34] Among the leading causes of vision loss Carbon quantum dots (CDs) are a class of emerging carbonaceous nanomaterials that have received considerable attention due to their excellent fluorescent properties, extremely small size, ability to penetrate cells and tissues, ease of synthesis, surface modification, low cytotoxicity, and superior water dispersion. In light of these properties, CDs are extensively investigated as candidates for bioimaging probes, efficient drug carriers, and disease diagnostics. Functionalized CDs represent a promising therapeutic candidate for ocular diseases. Here, this work reviews the potential use of functionalized CDs in the diagnosis and treatment of eye-related diseases, including the treatment of macular and anterior segment diseases, as well as targeting Aβ amyloids in the retina.

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Antibacterial activity of 3D printed thermoplastic elastomers doped with carbon quantum dots for biomedical applications

Shaalan,

Vykydalová,

Švajdlenková

et al. 2024

Polym. Bull.

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Abstract3D printing provides a lot of varieties for the manufacturing of personalized biomedical devices. Incorporation of the nanoparticles with potential antibacterial activity to the printed materials is another added value. One example of such nanoparticles are hydrophobic carbon quantum dots (hCQDs), which are zero-dimensional redox-active materials with high chemical stability and low production costs. They produce singlet oxygen only when activated by a specific wavelength of visible blue light which allows for controlled antibacterial action and minimizes the chances of bacterial resistance emergence. We prepared and characterized polymer composites based on thermoplastic elastomers (TPE) doped with hCQDs (TPE/hCQDs). The composites were 3D printed using fused deposition modeling method. In the first set of samples, a filament of pure TPE was immersed in a solution of hCQDs (0.5 mg/mL), then 3D printed, and compared with unmodified TPE filament. The mechanical properties, swelling behavior, hardness, and thermal stability of TPE/hCQDs were compared with the pure TPE printed samples. The production of singlet oxygen was confirmed by the electron paramagnetic resonance method. The antibacterial activity of the samples was tested according to ISO 22196 against Staphylococcus aureus and Escherichia coli after one hour of exposure to blue light, which completely inhibited bacterial growth. Besides, the cytotoxicity of samples was evaluated by MTT assay, and no significant effect of the materials on cell viability was observed. 3D printed materials with antibacterial activity represent a perspective for the future, especially in the field of personalized medicine, as well as in products for other industries.

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Anti-aggregation effect of carbon quantum dots on diabetogenic and beta-cell cytotoxic amylin and beta amyloid heterocomplexes

Abstract: Pancreatic islet amyloid deposition is a pathological hallmark of Type 2 diabetes (T2D), contributing to the reduced functional -cell mass. Islet amyloids do not only result only from the aggregation...

Cited by 6 publications

References 31 publications

Nanoparticles and Nanocolloidal Carbon: Will They Be the Next Antidiabetic Class That Targets Fibrillation and Aggregation of Human Islet Amyloid Polypeptide in Type 2 Diabetes?

Nanoparticles and Nanocolloidal Carbon: Will They Be the Next Antidiabetic Class That Targets Fibrillation and Aggregation of Human Islet Amyloid Polypeptide in Type 2 Diabetes?

Functional Carbon Quantum Dots for Ocular Imaging and Therapeutic Applications

Antibacterial activity of 3D printed thermoplastic elastomers doped with carbon quantum dots for biomedical applications

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