Anti‐adipogenic effects of Garcinia extract on the lipid droplet accumulation and the expression of transcription factor

Kim, Myung-Sunny; Kim, Jin‐Kyung; Kwon, Dae-Young; Park, Raekil

doi:10.1002/biof.5520220140

Cited by 46 publications

(38 citation statements)

References 9 publications

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“…The previous study reported that the Garcinia cambogia extracts inhibits lipid droplet accumulation in fat cells without affecting adipose conversion in 3T3-L1 cells [67]. In addition, Kim et al reported that Garcinia cambogia extracts inhibits the cytoplasmic lipid droplet accumulation as well as adipogenic differentiation of pre-adipocytes [68]. Consistent with the reduction of lipid droplet, our results showed that (-)-HCA treated significantly decreased the triglyceride content in primary chicken hepatocytes.…”

Section: Discussionsupporting

confidence: 80%

(-)-Hydroxycitric Acid Reduced Lipid Droplets Accumulation Via Decreasing Acetyl-Coa Supply and Accelerating Energy Metabolism in Cultured Primary Chicken Hepatocytes

Li¹,

Peng²,

Ge³

et al. 2017

Cell Physiol Biochem

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Background/Aims: (-)-Hydroxycitric acid (HCA) had been shown to suppress fat accumulation in animals and humans, while the underlying biochemical mechanism is not fully understood, especially little information is available on whether (-)-HCA regulates energy metabolism and consequently affects fat deposition. Methods: Hepatocytes were cultured for 24 h and then exposed to (-)-HCA (0, 1, 10, 50 µM), enzyme protein content was determined by ELISA; lipid metabolism gene mRNA levels were detected by RT-PCR. Results: (-)-HCA significantly decreased the number and total area of lipid droplets. ATP-citrate lyase, fatty acid synthase and sterol regulatory element binding protein-1c mRNA level were significantly decreased after (-)-HCA treatment, whereas peroxisome proliferator-activated receptor α mRNA level was significantly increased. (-)-HCA significantly decreased ATP-citrate lyase activity and acetyl-CoA content in cytosol, but significantly increased glucose consumption and mitochondrial oxygen consumption rate. (-)-HCA promoted the activity/content of glucokinase, phosphofructokinase-1, pyruvate kinase, pyruvate dehydrogenase, citrate synthase, aconitase, succinate dehydrogenase, malate dehydrogenase, NADH dehydrogenase and ATP synthase remarkably. Conclusions: (-)-HCA decreased lipid droplets accumulation by reducing acetyl-CoA supply, which mainly achieved via inhibition of ATP-citrate lyase, and accelerating energy metabolism in chicken hepatocytes. These results proposed a biochemical mechanism of fat reduction by (-)-HCA in broiler chickens in term of energy metabolism.

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Section: Discussionsupporting

confidence: 80%

(-)-Hydroxycitric Acid Reduced Lipid Droplets Accumulation Via Decreasing Acetyl-Coa Supply and Accelerating Energy Metabolism in Cultured Primary Chicken Hepatocytes

Li¹,

Peng²,

Ge³

et al. 2017

Cell Physiol Biochem

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“…These results are in agreement with the previous observation by other investigators that the inhibitory effect of the Garcinia cambogia extract against the insulin-induced differentiation of 3T3-L1 cells targeted the adipogenic transcription factor, C/ EBP. 45) Furthermore, the result that the expression of the TNF gene, which was up-regulated by HFD, was down-regulated again by the Garcinia cambogia extract supplemented to HFD corresponds well with the dietinduced changes in the expression of such other genes as PPAR2, C/EBP, and SREBP1c. Therefore, the Garcinia cambogia extract appears to have modulated the expression of a critical nuclear transcription factor that can trigger the entire process of adipocyte differentiation.…”

Section: Discussionmentioning

confidence: 57%

Garcinia cambogiaExtract Ameliorates Visceral Adiposity in C57BL/6J Mice Fed on a High-Fat Diet

Kim

Lee

Kim

et al. 2008

Bioscience, Biotechnology, and Biochemistry

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“…Although we did not measure energy expenditure, but an increasing in feed intake provides indirect evidence for that rats supplemental with Garcinia Cambogia extracts were less efficient in using the food energy for the accretion of body mass, which suggests that energy expenditure was increased. Previous studies also reported that the Garcinia Cambogia extracts could remarkably reduce body weight gain, visceral fat accumulation, and serum lipid concentration in high-fat diet-induced obesity rats (Kim et al, 2008;Kim et al, 2004).…”

Section: Numerous Studies Have Shown An Association Between Garciniamentioning

confidence: 81%

“…This observation concurs with the finding of Kim et al (Kim et al, 2007), who reported that Garcinia Cambogia extracts could reduce the accumulation of visceral fat mass and triglyceride content in rats rendered obese by a high fat diet. As a weight loss agent, many studies had reported that (-)-HCA, the principal acid and the major active ingredient of Garcinia Cambogia extracts, safely promote weight loss in laboratory animals (Kang et al, 2007;Kim et al 2004) and humans (Kim et al, 2011;Márquez, 2012), and suggested food intake regulation may be a major mechanism of weight loss by Garcinia Cambogia extracts-induced (Louter-van de Haar et al, 2005). Present study showed that supplemental with median and high dose of Garcinia Cambogia extracts could increase the average daily feed intake was observed in rats under fed high-fat diet.…”

Section: Numerous Studies Have Shown An Association Between Garciniamentioning

confidence: 99%

<i>Garcinia Cambogia</i> Extracts Prevented Fat Accumulation via Adiponectin-AMPK Signaling Pathway in Developing Obesity Rats

Liu

Han

et al. 2015

FSTR

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AbbreviationsACL, ATP-citrate lyase; ACO, Acyl-CoA oxidase; AdipoR1, adiponectin receptor 1; AdipoR2, adiponectin receptor 2; AMPK, adenosine 5'-monophosphate-actvated protein kinase; ATGL, adipose triglyceride lipase; BMI, body mass index; CPT-I, carnitine palmitoyltransferase-I; FAS, fatty acid synthesis; HCA, hydroxycitric acid; HDL, high-density lipoprotein; HL, hepatic lipase; NEFA, nonesterified fatty acid; TC, total cholesterol; TG, triglycerol. Original paper Garcinia Cambogia Extracts Prevented Fat Accumulation via Adiponectin- AMPK Signaling Pathway in Developing Obesity RatsGuanxing Liu, Ningning Han, Jing Han, Di CHen, Jian Kang and Haitian Ma IntroductionObesity is a public health problem which is fast growing throughout the modern world. It is a serious risk factor for so-called lifestyle-related diseases such as diabetes, cardiovascular disease and hypertension (Jebb and Moore, 1999;Nakamura et al., 1994). In addition to exercise, it is well known that anti-obesity foods and food ingredients could control and reduce body weight. Some herbal products and plant extracts, such as Semen Cassiaem (Junbao et al., 2004), Panax ginseng berry extract (Attele et al., 2002), Singiber officinale Roscoe (Hasegawa, 2001), and Platycodi radix (Han et al., 2000) have been shown to exert anti-obesity effects in rodents with high fat diet induced obesity (Kim et al., 2007).Garcinia cambogia is an edible fruit native to Southeast Asia (Saito et al., 2005). Its dried rind has been used for centuries throughout Southeast Asia as a food preservative, flavoring agent and carminative. Also, Garcinia cambogia extracts has been used as an ingredient of dietary supplements for weight loss.(-)-Hydroxycitric acid (HCA), a primary acid and the major active G. Liu et al. 836 ingredient in the fruit rinds of Garcinia Cambogia (Lewis and Neelakantan, 1965), has been shown to suppress appetite, body weight gain and fat accumulation in both experimental animals and humans (Greenwood et al., 1981;Heymsfield et al., 1998;Ohia, 2002;Rao and Sakariah, 1988;Sullivan et al., 1974;. HCA had been identified as a potent competitive inhibitor of the extra-mitochondrial enzyme adenosine triphosphate-citratelyase (Watson and Lowenstein, 1970), which catalyzes the cleavage of citrate in extra-mitochondrial to oxaloacetate and acetyl-CoA, a building block of fatty acids synthesis (Márquez, 2012). The inhibitory action of HCA induces the reduction of acetyl-CoA content, thus limiting the biosynthesis of fatty acids and cholesterol (Lowenstein, 1971;Sullivan et al., 1974). Also, the reduction of acetyl-CoA content could decrease the concentration of malonyl-CoA, which stimulates carnitine palmitoyltransferase I (CPT-1) activity and fatty acid oxidation (Ishihara et al., 2000;McCarty, 1994;Saha et al., 1999). Although some studies had revealed that HCA may suppress de novo fatty acid biosynthesis and appetite (Kovacs and Westerterp-Plantenga, 2006) and increase energy expenditure (Kim et al., 2008), subsequently reducing fat accumulation and b...

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Anti‐adipogenic effects of Garcinia extract on the lipid droplet accumulation and the expression of transcription factor

Cited by 46 publications

References 9 publications

(-)-Hydroxycitric Acid Reduced Lipid Droplets Accumulation Via Decreasing Acetyl-Coa Supply and Accelerating Energy Metabolism in Cultured Primary Chicken Hepatocytes

(-)-Hydroxycitric Acid Reduced Lipid Droplets Accumulation Via Decreasing Acetyl-Coa Supply and Accelerating Energy Metabolism in Cultured Primary Chicken Hepatocytes

Garcinia cambogiaExtract Ameliorates Visceral Adiposity in C57BL/6J Mice Fed on a High-Fat Diet

<i>Garcinia Cambogia</i> Extracts Prevented Fat Accumulation via Adiponectin-AMPK Signaling Pathway in Developing Obesity Rats

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