Anti-adipogenic Constituents from <i>Dioscorea opposita</i> in 3T3-L1 Cells

Abstract

We previously reported the lipase inhibitory activity of the n-BuOH fraction of Dioscorea opposita (DOB) and its isolates. This study sought to evaluate their anti-adipogenic activity in terms of their effects on the adipogenic transcription factors peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer binding protein α (C/EBPα) as well as phosphorylated AMP-activated protein kinase (p-AMPK) and carnitine palmitoyl transferase-1 (CPT-1). DOB apparently attenuated 3T3-L1 adipocyte differentiation (33.6% decrease at 20 µg/mL). In addition, a marked decrease (90.4%) in the expression of PPARγ was observed in the DOB-treated 3T3-L1 cells. Four isolates from DOB: (4E,6E)-1,7-bis(4-hydroxyphenyl)-4,6-heptadien-3-one (1), (3R,5R)-1,7-bis(4-hydroxy-3-methoxyphenyl)-3,5-heptanediol (2), batatasin I (3), and (1E,4E,6E)-1,7-bis(4-hydroxyphenyl)-1,4,6-heptatrien-3-one (4), suppressed adipocyte differentiation by inhibiting PPARγ at 20 µM (85.9%, 68.6%, 76.2%, and 90.2% decrease, respectively) and C/EBPα (51.7%, 3.1%, 20.9%, and 59.8% decrease, respectively). Batatasin I was found to increase p-AMPK and CPT-1 at a concentration of 20 µM in 3T3-L1 adipocytes, resulting in inhibiting adipogenesis. Taken together, batatasin I might be responsible for the anti-adipogenic effect of DOB via inhibition of PPARγ and C/EBPα and activation of p-AMPK and CPT-1.

α-Glucosidase Inhibitory Activity and Anti-Adipogenic Effect of Compounds from Dendrobium delacourii

“…According to previous report about anti-adipogenic activity of phenolic compound, batatasin I, at 20 µM [40], the suppressive effect of compounds (1-11) isolated from ethyl acetate extract of D. delacourii at the same concentration (20 µM) was preliminarily demonstrated by the remarkable reduction in both oil red O staining percentage (Figure 4a) and oil red O staining cells (Figure 4b) in differentiated 3T3-L1 cells. Although ephemeranthoquinone (2) and densifloral B (3) showed the highest anti-adipogenic activity among various isolates as well as positive control (oxyresveratrol), with approximately 64.7% and 65.2% reduction in oil red O staining, respectively, compound 2 at 20 µM significantly decreased viability in 3T3-L1 cells (data not shown).…”

“…Then, the differentiation media was replaced with culture media containing 5 µg/mL of insulin. After further incubation for 2 days, the cells were maintained in complete DMEM, which was changed every 2 days until adipocytes containing lipid droplets were observed under microscope [40].…”

Anti-obesity Effect of <i>Dioscorea oppositifolia</i> Extract in High-Fat Diet-Induced Obese Mice and Its Chemical Characterization

“…8) In addition, the anti-adipogenesis effect of D. oppositifolia n-BuOH soluble extract has been detected in cultured 3T3-L1 adipocyte. 9) Biological activities of Dioscorea species against diverse metabolic disorders including diabetes and obesity have been already documented. 10,11) However, anti-obesity effect of certain extract derived from D. oppositifolia in animal model remains to be defined.…”

Identifying of Anti-Thrombin Active Components From Curcumae Rhizoma by Affinity-Ultrafiltration Coupled With UPLC-Q-Exactive Orbitrap/MS

“…C14 suppressed adipocyte differentiation by inhibiting PPARγ. C/EBPα inhibits the differentiation of 3T3-L1 adipocytes (Yang et al, 2014a) and reduced pancreatic lipase activity at low concentrations (Yang et al, 2014b).…”