Anti-adhesion and antibacterial activity of silver nanoparticles supported on graphene oxide sheets

Faria, Andréia Fonseca de; Martinez, Diego Stéfani T.; Meira, Stela Maris Meister; Moraes, Ana Carolina Mazarin de; Brandelli, Adriano; Filho, A. G. Souza; Alves, Oswaldo Luiz

doi:10.1016/j.colsurfb.2013.08.006

Cited by 352 publications

(250 citation statements)

References 41 publications

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“…For example, attachment of AgNPs on the surface of GO sheets can prevent AgNPs from aggregating, allowing a more controlled release of AgNPs + ions, which eventually can increase antibacterial and anticancer activity. 21,37,[39][40][41][42] Recent studies on basic and translational cancer have been focused on development of novel therapeutic agents targeting multidimensional aspects that contribute to the development and progression of cancers and the resistance of tumors to conventional therapies. 43,44 Although several studies have addressed the molecular mechanism of cancer pathogenesis, the process of understanding remains extremely complex, and much is still unknown because of intrinsic resistance and loss of sensitivity to a therapeutic agent.…”

mentioning

confidence: 99%

Quercetin-mediated synthesis of graphene oxide–silver nanoparticle nanocomposites: a suitable alternative nanotherapy for neuroblastoma

Yuan¹,

Wang²,

Xing³

et al. 2017

IJN

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Background Graphene and graphene-related materials have gained substantial interest from both academia and industry for the development of unique nanomaterials for biomedical applications. Graphene oxide (GO) and silver nanoparticles (AgNPs) are a valuable platform for the development of nanocomposites, permitting the combination of nanomaterials with different physical and chemical properties to generate novel materials with improved and effective functionalities in a single platform. Therefore, this study was conducted to synthesize a graphene oxide–silver nanoparticle (GO-AgNPs) nanocomposite using the biomolecule quercetin and evaluate the potential cytotoxicity and mechanism of GO-AgNPs in human neuroblastoma cancer cells (SH-SY5Y). Methods The synthesized GO-AgNPs were characterized using various analytical techniques. The potential toxicities of GO-AgNPs were evaluated using a series of biochemical and cellular assays. The expression of apoptotic and anti-apoptotic genes was measured by quantitative real-time reverse transcription polymerase chain reaction. Further, apoptosis was confirmed by caspase-9/3 activity and a terminal deoxynucleotidyl transferase dUTP nick end labeling assay, and GO-AgNPs-induced autophagy was also confirmed by transmission electron microscopy. Results The prepared GO-AgNPs exhibited significantly higher cytotoxicity toward SH-SY5Y cells than GO. GO-AgNPs induced significant cytotoxicity in SH-SY5Y cells by the loss of cell viability, inhibition of cell proliferation, increased leakage of lactate dehydrogenase, decreased level of mitochondrial membrane potential, reduced numbers of mitochondria, enhanced level of reactive oxygen species generation, increased expression of pro-apoptotic genes, and decreased expression of anti-apoptotic genes. GO-AgNPs induced caspase-9/3-dependent apoptosis via DNA fragmentation. Finally, GO-AgNPs induced accumulation of autophagosomes and autophagic vacuoles. Conclusion In this study, we developed an environmentally friendly, facile, dependable, and simple method for the synthesis of GO-AgNPs nanocomposites using quercetin. The synthesized GO-AgNPs exhibited enhanced cytotoxicity compared with that of GO at very low concentrations. This study not only elucidates the potential cytotoxicity against neuroblastoma cancer cells, but also reveals the molecular mechanism of toxicity.

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mentioning

confidence: 99%

Quercetin-mediated synthesis of graphene oxide–silver nanoparticle nanocomposites: a suitable alternative nanotherapy for neuroblastoma

Yuan¹,

Wang²,

Xing³

et al. 2017

IJN

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“…Dispersion of GO showed negligible antimicrobial activity against the microorganism and GO-Ag compound showed an increased antibacterial activity. The activity for antibiofilm was also observed on Pseudomonas aeruginosa, on a stainless steel surface showing 100% inhibition rate when exposed to the GO-Ag nanocomposite [87,88]. Bao et al [89] had a similar result using a disc diffusion test and evaluated the antibacterial property of GO by observing an inhibition zone surrounding the GO disc showing toxicity of bacteria against Staphylococcus aureus and Escherichia.coli.…”

Section: Substrates For Antibacterial Effectsmentioning

confidence: 67%

Graphene and graphene oxide as nanomaterials for medicine and biology application

et al. 2018

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Graphene-and graphene oxide-based nanomaterials have gained broad interests in research because of their unique physiochemical properties. The 2D allotropic structure allows it to be used in various biological fields. The biomedical applications of graphene and its composite include its use in gene and small molecular drug delivery. It is further used for biofunctionalization of protein, in anticancer therapy, as an antimicrobial agent for bone and teeth implantation. The biocompatibility of the newly synthesized nanomaterials allows its substantial use in medicine and biology. The current review summarizes the chemical structure and biological application of graphene in various fields.

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“…The presence of the bigger particles can be due to agglomeration of the two or more particles together. Ag nanoparticles loaded on EG comes near to the size of those loaded on GO sheets [23], but smaller than those loaded on ACFs [38,39]. Figure 3c clearly shows the interface between the sheet and Ag, indicating that Ag nanoparticles are well attached between the surfaces of the sheet.…”

Section: Morphology Observation By Sem and Temmentioning

confidence: 85%

“…From the FT-IR spectra, it could be deduced that functional groups provide ideal nucleation sites for Ag nanoparticles; powerful interactions would occur between Ag nanoparticles and the un-reduced oxygen-containing groups on the surface of the GICs and that of the EG. Therefore, Ag nanoparticles can strongly attach to the exfoliated graphite surface [23].…”

Section: Xrd and Ftir Analysismentioning

confidence: 99%

“…Especially graphene, for AgNPs loading, is a favorable matrix because of their abundant functional groups and large surface areas. A work provides the first direct evidence that Ag-loaded graphene oxide (Ag-GO) nanocomposites can inhibit the growth of microbial adhered cells, thus preventing the process of biofilm formation [23]. Liu et al [24] and Das et al [25] demonstrated antibacterial activity for Ag-GO nanocomposites against Gram-negative Escherichia coli.…”

Section: Introductionmentioning

confidence: 99%

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Silver Nanoparticles-Loaded Exfoliated Graphite and Its Anti-Bacterial Performance

Hou

Huang

et al. 2017

Applied Sciences

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Abstract:One antibacterial material was prepared from exfoliated graphite (EG) decorated with silver nanoparticles (AgNPs). The EG was prepared by the graphite intercalated compound process, AgNPs were prepared by chemical reduction of AgNO 3 in the presence of NaBH 4 . The AgNPs-loaded EG (Ag-EG) composite was characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), nitrogen adsorption, mercury intrusion porosimetry, scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The antibacterial effect of the Ag-EG was evaluated by using the zone of inhibition method. The loaded AgNPs were highly dispersed on EG sheets and most of them have a size less than 10 nm. The Ag loading slightly increased the surface area of EG. It is shown that the Ag-EG had antibacterial activity and anti-adhesion properties against Pseudomonas aeruginosa and Staphylococcus aureus. It suggests that Ag-EG composites could be used in a variety of industrial applications that require an antibacterial effect.

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Anti-adhesion and antibacterial activity of silver nanoparticles supported on graphene oxide sheets

Cited by 352 publications

References 41 publications

Quercetin-mediated synthesis of graphene oxide–silver nanoparticle nanocomposites: a suitable alternative nanotherapy for neuroblastoma

Quercetin-mediated synthesis of graphene oxide–silver nanoparticle nanocomposites: a suitable alternative nanotherapy for neuroblastoma

Graphene and graphene oxide as nanomaterials for medicine and biology application

Silver Nanoparticles-Loaded Exfoliated Graphite and Its Anti-Bacterial Performance

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Anti-adhesion and antibacterial activity of silver nanoparticles supported on graphene oxide sheets

Cited by 352 publications

References 41 publications

Quercetin-mediated synthesis of graphene oxide&ndash;silver nanoparticle nanocomposites: a suitable alternative nanotherapy for neuroblastoma

Quercetin-mediated synthesis of graphene oxide&ndash;silver nanoparticle nanocomposites: a suitable alternative nanotherapy for neuroblastoma

Graphene and graphene oxide as nanomaterials for medicine and biology application

Silver Nanoparticles-Loaded Exfoliated Graphite and Its Anti-Bacterial Performance

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Quercetin-mediated synthesis of graphene oxide–silver nanoparticle nanocomposites: a suitable alternative nanotherapy for neuroblastoma

Quercetin-mediated synthesis of graphene oxide–silver nanoparticle nanocomposites: a suitable alternative nanotherapy for neuroblastoma