Anti-Adenovirus Activity of Epiandrosterone and Dehydroepiandrosterone Derivatives

Romanutti, Carina; Bruttomesso, Andrea C.; Castilla, Viviana; Galagovsky, Lydia R.; Wachsman, Mónica B.

doi:10.1159/000313530

Cited by 18 publications

(11 citation statements)

References 80 publications

(48 reference statements)

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“…Antiherpetic activity has been shown for aqueous plant extracts from Echinacea pallidum [32], Rumex acetosa [33] and Rhododendron ferrugineum [34], for polysaccharides derived from marine algae [35], for steroidal compounds against adenovirus [36] and an ethylacetate-soluble fraction from the lichen Ramalina farinacea against HIV [37]. Although all analyzed drugs in our study demonstrated virucidal activity, Melissa extract demonstrated a higher virucidal activity when compared to single compounds.…”

Section: Discussionmentioning

confidence: 62%

<i>Melissa officinalis</i> Extract Inhibits Attachment of Herpes Simplex Virus in vitro

Astani

Reichling²,

Schnitzler³

2012

Chemotherapy

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Background: Extracts and essential oils of medicinal plants are increasingly of interest as novel drugs for antiherpetic agents, since the herpes simplex virus (HSV) might develop resistance to commonly used antiviral drugs. Methods: An aqueous extract of Melissa officinalis as well as phenolic extract compounds, i.e. caffeic acid, p-coumaric acid and rosmarinic acid were examined for their antiviral activity against herpes simplex virus type 1 (HSV-1) in vitro. Results: When drugs were added to HSV-1-infected cells, no antiviral effect was observed as determined by plaque reduction assay and analysis of expression of viral protein ICP0. However, the Melissa extract demonstrated a high virucidal activity against HSV-1, even at very low concentrations of 1.5 µg/ml, whereas similar results for phenolic compounds were only achieved at 100 times higher concentrations. Besides the virucidal activity, the Melissa extract and rosmarinic acid inhibited HSV-1 attachment to host cells in a dose-dependent manner. These results indicate that rosmarinic acid was the main contributor to the antiviral activity of Melissa extract. However, the selectivity index of Melissa extract of 875 against HSV is superior to the selectivity indices of single constituents. Conclusion: Melissa extract exhibits low toxicity, is virucidal and affects HSV-1 attachment to host cells in vitro.

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Section: Discussionmentioning

confidence: 62%

<i>Melissa officinalis</i> Extract Inhibits Attachment of Herpes Simplex Virus in vitro

Astani

Reichling²,

Schnitzler³

2012

Chemotherapy

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“…The binding affinity between NAIs and neuraminidase as well as the route of NAI administration might explain the differences between the NAIs in the time taken for the alleviation of symptoms and fever. If NAIs had the same antiviral activities against influenza virus as DEAE affected adenovirus protein synthesis and mature particle formation [20], there was no difference in time between the alleviation of symptoms and fever. Further investigation is required to determine whether or not a rapid increase in the neuraminidase concentration from the start of medical intervention would be a more effective treatment option.…”

Section: Discussionmentioning

confidence: 99%

Clinical Effects of Oseltamivir, Zanamivir, Laninamivir and Peramivir on Seasonal Influenza Infection in Outpatients in Japan during the Winter of 2012-2013

Takemoto¹,

Asai²,

Ikezoe³

et al. 2013

Chemotherapy

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Background: The neuraminidase inhibitors (NAIs) oseltamivir, zanamivir, laninamivir and peramivir are available in Japan. However, the selective use of NAIs for treating outpatients with influenza has not been clearly defined. Methods: We assigned 191 patients with influenza to 4 groups, each treated with a different NAI, and then compared how long it took to alleviate fever and other symptoms and to eliminate the virus. Results: Alleviation of fever occurred significantly sooner with peramivir than with either zanamivir (p = 0.0002) or oseltamivir (p = 0.0059), but was not significantly different from that with laninamivir (p = 0.0457; p < 0.0083). Other symptoms were also alleviated sooner by peramivir than by the other 3 NAIs. Conclusions: The ability of each NAI to alleviate influenza symptoms and fever varied. The appropriate use of NAIs requires further study.

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“…Considering the lack of a secure and effective therapy against MV, research into finding new antiviral drugs is of great importance. Although DHEA has been attributed with many beneficial properties and antiviral activity against other viruses, the precise mechanism of action of DHEA remains unclear [41,48,49]. In this study we found that DHEA is active against MV in a dose-dependent manner.…”

Section: Discussionmentioning

confidence: 46%

“…This precise MTT procedure has been previously described [40] and the cytotoxicity of DHEA on growing cells was determined using the MTT assay described previously [41]. The compound concentration (µM) that reduced cell viability by 50% of the control, calculated by regression analysis, was defined as CC 50 .…”

Section: Cytotoxicity Assaysmentioning

confidence: 99%

DHEA inhibits Measles Virus through a Mechanism Independent of its ability to Modulate the Raf/MEK/ERK Signaling Pathway

2012

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Aim: Despite the existence of an effective vaccine, measles infection is still frequent in many developing countries with reduced health infrastructure, and it is one of the major causes of child death globally. In the past decade numerous outbreaks have occurred in developed countries, giving a fresh impetus to antiviral research against measles virus. The aim of this study was to investigate the antiviral activity of the natural steroid hormone DHEA against measles virus and the role of the Raf/MEK/ERK signaling pathway in viral multiplication and DHEA’s antiviral activity. Materials & methods: The antiviral activity of DHEA and two ERK modulators, UO126 and anisomycin, was determined using a virus yield reduction assay. Furthermore, we studied DHEA’s virucidal activity and the viral multiplication step affected by the compound. The effect of virus infection on the Raf/MEK/ERK pathway and the activity of those compounds against measles virus spread and induced cytopathic effect were studied using western blot and indirect immunofluorescence. Results & conclusion: We found that DHEA and UO126 are active against measles virus and that they are able to diminish virus-induced cytopathic effects. Also, our study showed that early events in the viral multiplication cycle trigger ERK activation, suggesting that DHEA, a Raf/MEK/ERK modulator, may not exert its antiviral activity through the modulation of this pathway. Our results may provide a first step in the development of new antiviral agents against measles virus.

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Anti-Adenovirus Activity of Epiandrosterone and Dehydroepiandrosterone Derivatives

Cited by 18 publications

References 80 publications

<i>Melissa officinalis</i> Extract Inhibits Attachment of Herpes Simplex Virus in vitro

<i>Melissa officinalis</i> Extract Inhibits Attachment of Herpes Simplex Virus in vitro

Clinical Effects of Oseltamivir, Zanamivir, Laninamivir and Peramivir on Seasonal Influenza Infection in Outpatients in Japan during the Winter of 2012-2013

DHEA inhibits Measles Virus through a Mechanism Independent of its ability to Modulate the Raf/MEK/ERK Signaling Pathway

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