Anti-ACTL7a antibodies: a cause of infertility

Fu, Jun; Wang, Yong; Fok, Kin Lam; Yang, Dantong; Yi, Qiu; Chan, Hsiao Chang; Koide, S. S.; Miao, Shiying; Wang, Linfang

doi:10.1016/j.fertnstert.2012.02.023

Cited by 25 publications

(26 citation statements)

References 37 publications

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“…Slices from mouse testes were prepared and analysed by IHC as previously described [18]. Frozen sections from mouse testes were used for subsequent IF analyses, which were performed according to procedures previously described [19].…”

Section: Methodsmentioning

confidence: 99%

Nemo‐like kinase promotes etoposide‐induced apoptosis of male germ cell‐derived GC‐1 cells in vitro

et al. 2012

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a b s t r a c tSpermatogenesis is an extremely intricate process that is tightly regulated and orchestrated by a series of well-coordinated gene expression programmes. Nemo-like kinase (NLK) is an evolutionarily conserved serine/threonine kinase that functions in a wide variety of developmental events. Nevertheless, the function of NLK in spermatogenesis has not been investigated. In this study, we found that the distribution of NLK in mice exhibited a dynamic change during testicular development and gradually became concentrated in the acrosomes of elongated spermatids. NLK overexpression promoted etoposide-induced apoptosis of male germ cell-derived GC-1 cells, while knockdown of NLK by RNA interference (RNAi) attenuated etoposide-induced apoptosis. Our findings suggest that NLK plays an important role in etoposide-induced germ cell apoptosis and may be associated with spermatogenesis.

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Section: Methodsmentioning

confidence: 99%

Nemo‐like kinase promotes etoposide‐induced apoptosis of male germ cell‐derived GC‐1 cells in vitro

et al. 2012

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“…Within 282-287 Mb are located the Actin Like 7A (ACTL7A), Actin Like 7B (ACTL7B), Catenin Alpha Like 1 (CTNNAL1), and Prostaglandin Reductase 1 (PTGR1) genes. ACTL7A and ACTL7B play functions related to capacitation of spermatozoa and fertility in mice [51,52]. In Large White sows, it has been shown that CTNN AL1 is associated with litter size in pigs [53,54].…”

Section: Genome-wide Association Analysismentioning

confidence: 99%

Genetic and genomic characterization of vulva size traits in Yorkshire and Landrace gilts

Corredor

Sanglard

Leach³

et al. 2020

BMC Genet

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Background: Reproductive performance is critical for efficient swine production. Recent results indicated that vulva size (VS) may be predictive of reproductive performance in sows. Study objectives were to estimate genetic parameters, identify genomic regions associated, and estimate genomic prediction accuracies (GPA) for VS traits. Results: Heritability estimates of VS traits, vulva area (VA), height (VH), and width (VW) measurements, were moderately to highly heritable in Yorkshire, with 0.46 ± 0.10, 0.55 ± 0.10, 0.31 ± 0.09, respectively, whereas these estimates were low to moderate in Landrace, with 0.16 ± 0.09, 0.24 ± 0.11, and 0.08 ± 0.06, respectively. Genetic correlations within VS traits were very high for both breeds, with the lowest of 0.67 ± 0.29 for VH and VW for Landrace. Genome-wide association studies (GWAS) for Landrace, reveled genomic region associated with VS traits on Sus scrofa chromosome (SSC) 2 (154-157 Mb), 7 (107-110 Mb), 8 (4-6 Mb), and 10 (8-19 Mb). For Yorkshire, genomic regions on SSC 1 (87-91 and 282-287 Mb) and 5 (67 Mb) were identified. All regions explained at least 3.4% of the genetic variance. Accuracies of genomic prediction were moderate in Landrace, ranging from 0.30 (VH) to 0.61 (VA), and lower for Yorkshire, with 0.07 (VW) to 0.11 (VH). Between-breed and multi-breed genomic prediction accuracies were low. Conclusions: Our findings suggest that VS traits are heritable in Landrace and Yorkshire gilts. Genomic analyses show that major QTL control these traits, and they differ between breed. Genomic information can be used to increase genetic gains for these traits in gilts. Additional research must be done to validate the GWAS and genomic prediction results reported in our study.

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“…Recently antibodies against ACTL7a, a spermatozoan specific protein has been demonstrated to be the cause of immunologic infertility in males following vasectomy as proved by Fu et al [18] by mass spectrometry, direct immunostaining, sperm agglutination tests and standard infertility assays, where Anti ACTL7a antibodies may cause infertility in mice because in vitro treatment of mouse spermatozoa with ACTL7a antibody containing serum markedly reduced the fertilizing potential of the spermatozoa. In addition active immunization of mice with ACTL7a resulted in significant reduction in fertility.…”

Section: Introductionmentioning

confidence: 99%

“…The expression of ACTL7a is upregulated via PKA pathway and undergoes this during the early period of capacitation in mouse spermatozoa. Fu et al [18] further demonstrated how ACTL7a is an essential component of mouse capacitation and dynamic changes occur in the expression and localization of ACTL7a which maybe a primary biochemical event in the induction of capacitation in mouse spermatozoa [19].…”

Section: Introductionmentioning

confidence: 99%

An update on Immunologic Infertility-special Emphasis on Role of PD-1(Programmed Death 1) Gene

Kaur¹

2014

JFIV Reprod Med Genet

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The association of antisperm antibodies and autoimmunity or presence of gamma delta T cells in semen associated with men with ASA related infertility has been recognized as early as the early 90's with specific correlation with antiphospholipid antibodies but it has been concluded in a review in 2002 by Mclachlan RI 2002 that since IUI/ICSI is the final answer there is no point in bothering about looking or investigating for antisperm antibodies and hence not much interest was given till recently interest got rekindled by the appearance of a report on the presence of programed death 1(PD-1) gene polymorphisms in men with ASA related infertility as PD-1 is a negative costimulatory molecule also found in sertoli cells and associated with the transplantation potential or helping in the immune privileged site of testis as reported by Dal secco and hence this review is done to highlight the implications of getting insight into the reason for male immune infertility and its difference from female one and correlation with other autoimmune disorders and future therapeutic potentials including in recurrent mishaps as well as for future orthotopic testicular transplantation in men with testis in inguinal region other causes of immune infertility as simple as just subtle chlamydia infection.

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Anti-ACTL7a antibodies: a cause of infertility

Cited by 25 publications

References 37 publications

Nemo‐like kinase promotes etoposide‐induced apoptosis of male germ cell‐derived GC‐1 cells in vitro

Nemo‐like kinase promotes etoposide‐induced apoptosis of male germ cell‐derived GC‐1 cells in vitro

Genetic and genomic characterization of vulva size traits in Yorkshire and Landrace gilts

An update on Immunologic Infertility-special Emphasis on Role of PD-1(Programmed Death 1) Gene

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