Anti-Acetylcholinesterase and Antioxidant Assets of the Major Components (Salicin, Amentoflavone, and Chlorogenic Acid) and the Extracts ofViburnum opulusandViburnum lantanaand Their Total Phenol and Flavonoid Contents

Abstract

Some Viburnum species are used for preparation of the traditional drink called gilaburu in Anatolia. In the current study, our goal was to evaluate acetylcholinesterase (AChE) inhibitory and antioxidant activities of the ethyl acetate, methanol, and water extracts prepared from the branches, leaves, and fruits of Viburnum opulus and Viburnum lantana along with salicin, amentoflavone, and chlorogenic acid, three major compounds abundantly found in these species. AChE enzyme inhibition was tested in vitro using an enzyme-linked immunosorbent assay microplate reader at 50, 100, and 200 μL/mL concentrations. Antioxidant activity was examined by ferrous ion chelating capacity, ferric reducing antioxidant power, and β-carotene bleaching assay at 500, 1,000, and 2,000 μg/mL. Total phenol and flavonoid contents of the extracts were also established by Folin-Ciocalteau and AlCl(3) reagents, respectively. Our data revealed that the leaf methanol extract of V. opulus displayed a significantly high inhibitory effect against AChE (57.63 ± 1.23%, 87.41 ± 0.99%, and 93.19 ± 0.87% at 50, 100, and 200 μg/mL, respectively). The extracts of V. lantana exerted higher antioxidant activity.

Amentoflavone protects against high fat-induced metabolic dysfunction: Possible role of the regulation of adipogenic differentiation

“…A number of studies have demonstrawted that AMF exerts anti-inflammatory (17–20), antioxidant (25–27), anti-viral (28,29) and antitumor effects (30,31), as well as protective effects against radiation-induced damaqge (32,33) and neuroprotective effects (32,33). Na et al found that AMF inhibited protein tyrosine phosphatase 1B activity, implicating a potential use of AMF in the treatment of type 2 diabetes and obesity (21).…”

Preclinical Evaluation of Antiurolithiatic Activity ofViburnum opulusL. on Sodium Oxalate-Induced Urolithiasis Rat Model

“…The phenolic constituents of V. opulus were found to provide high antioxidant capacity [7]. In our previous studies the antioxidant activities of V. opulus and V. lantana were examined by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging and superoxide anion scavenging methods [22], ferrous ion chelating capacity, ferric reducing antioxidant power, and β -carotene bleaching assay [10]. Therefore, the antioxidant activity results of the present study were found to be in line with the previous data.…”

“…According to this study, V. opulus fruits are considered as a good chlorogenic acid source [7]. This genus was reported to possess antinociceptive [5], relaxant, spasmolytic [8], hepatoprotective, hypoglycemic [9], antioxidant [7, 10], antiacetylcholinesterase [10], and anticarcinogenic potential [11]. …”

Ratlarda Akciğer Transplantasyonda Iskemi/Reperfüzyonun İndüklediği Oksidatif Hasara Karşı Viburnum opulus Meyve Ekstresinin Koruyucu Etkilerinin Araştırılması

“…Viburnum opulus L. is known as "Gilaburu" in Turkey and "Guilder rose" in Europe [14] . V. opulus growing in Turkey have been so far reported to possess several biological activities, including antioxidant, hepatoprotective, hypoglycemic, antinociceptive, and antiinflammatory effects [15] . It has been traditionally used to prevent the formation of kidney stone [16] .…”

“…It has been traditionally used to prevent the formation of kidney stone [16] . Phenolic acids such as hydroxybenzoic acids, tannins, coumarins, cathechols, iridoid glycosides, antocyanins, ascorbic acid, chlorogenic acid, salicin, (+)-catechin, (-)-epicatechin, cyanidin-3 glucoside, cyanidin-3-rutinoside, and quercetin [15][16][17] were determined in the composition of V. opulus fruit extract.…”